Rabbit Recombinant Monoclonal BRAF35 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
Images
![Western blot - Anti-BRAF35 antibody [EPR9915] (AB167415), expandable thumbnail](https://content.abcam.com/products/images/braf35-antibody-epr9915-ab167415--western-blot-img126388.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BRAF35 antibody [EPR9915] (AB167415)")
![Immunocytochemistry/ Immunofluorescence - Anti-BRAF35 antibody [EPR9915] (AB167415), expandable thumbnail](https://content.abcam.com/products/images/braf35-antibody-epr9915-ab167415--immunocytochemistry-immunofluorescence-img16116.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-BRAF35 antibody [EPR9915] (AB167415)")
Publications
Filter by
- Molecular and cellular biology 39:2019Growth Factor Independence 1B-Mediated Transcriptional Repression and Lineage Allocation Require Lysine-Specific Demethylase 1-Dependent Recruitment of the BHC Complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid McClellan et. al.PubMed 30988160
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
Target data
Function
Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.
Alternative names
BRAF35, HMGX2, HMGXB2, SMARCE1R, HMG20B, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related, SMARCE1-related protein, BRCA2-associated factor 35, HMG box-containing protein 20B, HMG domain-containing protein 2, HMG domain-containing protein HMGX2, Sox-like transcriptional factor, Structural DNA-binding protein BRAF35
Recommended products
Rabbit Recombinant Monoclonal BRAF35 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR9915
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-BRAF35 antibody [EPR9915] (ab167415), expandable thumbnail](https://content.abcam.com/products/images/braf35-antibody-epr9915-ab167415--western-blot-img126388.jpg "Western blot - Anti-BRAF35 antibody [EPR9915] (ab167415)")
Western blot - Anti-BRAF35 antibody [EPR9915] (ab167415)
All lanes: Western blot - Anti-BRAF35 antibody [EPR9915] (ab167415) at 1/1000 dilution
Lane 1: Jurkat cell lysate at 10 µg
Lane 2: A431 cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP conjugated antibody
Predicted band size: 35 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-BRAF35 antibody [EPR9915] (ab167415), expandable thumbnail](https://content.abcam.com/products/images/braf35-antibody-epr9915-ab167415--immunocytochemistry-immunofluorescence-img16116.jpg "Immunocytochemistry/ Immunofluorescence - Anti-BRAF35 antibody [EPR9915] (ab167415)")
Immunocytochemistry/ Immunofluorescence - Anti-BRAF35 antibody [EPR9915] (ab167415)
Immunofluorescent analysis of A431 cells labeling BRAF35 with ab167415 at 1/100 dilution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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