AB322059

Anti-Brain-derived Tau antibody [EPR29150-21]

20ul selling size
BOND RX™ Validated
Recombinant
RabMAb
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Rabbit Recombinant Monoclonal TAU antibody. Suitable for WB, IHC-P, I-ELISA and reacts with Transfected cell lysate - Human, Human, Mouse, Transfected cell line - Human, Recombinant fragment - Human, Synthetic peptide - Mouse samples.

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

12 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Brain-derived Tau with ab322059 at 1/100 (4.98 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human cerebrum. The section was incubated with ab322059 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

Immunohistochemical analysis of paraffin-embedded Human Alzhiemer's disease brain tissue labeling Brain-derived Tau with ab322059 at 1/100 (4.98 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human Alzhiemer's disease brain. The section was incubated with ab322059 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a human Tau 2N4R expression vector containing a myc-His-tag®. (B) HEK-293T transfected with a human PNS-tau expression vector containing a DDDDK-tag. (C) HEK-293T transfected with empty vector containing a myc-His-tag®. tissue labeling Brain-derived Tau with ab322059 at 1/500 (0.996 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on HEK-293T transfected with a Myc-His-tagged human Tau 2N4R construct (image A). No staining on HEK-293T transfected with a DDDDK-tagged human PNS-tau construct (image B). No staining on HEK-293T transfected with empty plasmid (image C). The section was incubated with ab322059 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Brain-derived Tau with ab322059 at 1/100 (4.98 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human spleen. The section was incubated with ab322059 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Brain-derived Tau with ab322059 at 1/100 (4.98 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse spleen. The section was incubated with ab322059 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Brain-derived Tau with ab322059 at 1/100 (4.98 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse cerebrum. The section was incubated with ab322059 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

Immunohistochemical analysis of paraffin-embedded Mouse Alzhiemer's disease brain tissue labeling Brain-derived Tau with ab322059 at 1/100 (4.98 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse Alzhiemer's disease brain. The section was incubated with ab322059 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Western blot - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : kidney, spleen, liver (PMID : 24386422).

The molecular weight observed is consistent with what has been described in the literatures (PMID : 24386422, PMID : 3930508).

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

Exposure time : Lane 1-3 : 37 seconds; Lanes 4-6 : 8 seconds

All lanes:

Western blot - Anti-Brain-derived Tau antibody [EPR29150-21] (ab322059) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human spleen tissue lysate at 20 µg

Lane 3:

Human kidney tissue lysate at 20 µg

Lane 4:

Human brainstem tissue lysate at 20 µg

Lane 5:

Human hippocampus tissue lysate at 20 µg

Lane 6:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 37-70 kDa,124 kDa

false

Supplier Data

Western blot - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : kidney, spleen (PMID : 24386422).

The molecular weight observed is consistent with what has been described in the literatures (PMID : 24386422, PMID : 3930508).

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

Exposure time : Lane 1 : 15 seconds; Lanes 2-5 : 114 seconds

All lanes:

Western blot - Anti-Brain-derived Tau antibody [EPR29150-21] (ab322059) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse hippocampus tissue lysate at 20 µg

Lane 3:

Mouse cerebellum tissue lysate at 20 µg

Lane 4:

Mouse kidney tissue lysate at 20 µg

Lane 5:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37-70 kDa,124 kDa

false

Indirect ELISA - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

I-ELISA

Supplier Data

Indirect ELISA - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

Indirect ELISA analysis of ab322059 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : Mouse Tau 2N4R peptide,Mouse PNS-Tau protein.

Antigen concentration : 1000 ng/ml

This antibody does not cross-react with mouse PNS-Tau

I-ELISA

Supplier Data

Indirect ELISA - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

Indirect ELISA analysis of ab322059 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : Human Tau 2N4R protein,Human PNS-Tau protein.

Antigen concentration : 1000 ng/ml

This antibody does not cross-react with human PNS-Tau.

Supplier Data

Western blot - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human PNS-Tau.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

In Western blot, Anti-DDDDK-tag antibody staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Brain-derived Tau antibody [EPR29150-21] (ab322059) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human Tau 2N4R expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human PNS-tau expression vector containing a DDDDK-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37-70 kDa,36 kDa

false

Exposure time: 3s

Carrier free

Anti-Brain-derived Tau antibody [EPR29150-21] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29150-21

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

I-ELISA, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This clone recognized all brain-derived tau isoforms (Tau 0N3R, Tau-A, Tau 1N3R, Tau 2N3R, Tau 0N4R, Tau 1N4R and Tau 2N4R).
Not suitable for WB in human and mouse cell lines.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "1000 ng/mL", "IELISA-species-notes": "" }, "Synthetic peptide - Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "1000 ng/mL", "IELISA-species-notes": "" }, "Transfected cell line - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

See full target information MAPT

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Brain-derived Tau antibody [EPR29150-21]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

Western blot - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

Western blot - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

Indirect ELISA - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

Indirect ELISA - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

Western blot - Anti-Brain-derived Tau antibody [EPR29150-21] (AB322059)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Assay Kits

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise