Anti-BRAP antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-BRAP antibody (AB237505)

PC-3 (Human prostate adenocarcinoma cell line) cells stained for BRAP (green) using ab237505 at 1/100 dilution in ICC/IF. Secondary antibody is an Alexa Fluor^® 488-conjugated Goat Anti-Rabbit IgG (H+L). The nuclear counter stain is DAPI (blue).

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAP antibody (AB237505)

Paraffin-embedded human small intestine tissue stained for BRAP using ab237505 at 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAP antibody (AB237505)

Paraffin-embedded human colon cancer tissue stained for BRAP using ab237505 at 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• WB

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Western blot - Anti-BRAP antibody (AB237505)

All lanes:

Western blot - Anti-BRAP antibody (ab237505) at 1/500 dilution

Lane 1:

K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate

Lane 2:

Rat brain lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 67 kDa

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