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AB181868

Anti-BRAT1 antibody [EPR13753-43] - C-terminal

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(1 Publication)

Rabbit Recombinant Monoclonal BRAT1 antibody. C-terminal. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

BAAT1, C7orf27, BRAT1, BRCA1-associated ATM activator 1, BRCA1-associated protein required for ATM activation protein 1

3 Images
Flow Cytometry (Intracellular) - Anti-BRAT1 antibody [EPR13753-43] - C-terminal (AB181868)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-BRAT1 antibody [EPR13753-43] - C-terminal (AB181868)

Intracellular flow cytometric analysis of 293 cells (paraformaldehyde-fixed, 2%) labeling BRAT1with ab181868 at 1/100 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Immunoprecipitation - Anti-BRAT1 antibody [EPR13753-43] - C-terminal (AB181868)
  • IP

Supplier Data

Immunoprecipitation - Anti-BRAT1 antibody [EPR13753-43] - C-terminal (AB181868)

Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab181868 at 1/50 dilution.
Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-BRAT1 antibody [EPR13753-43] - C-terminal (ab181868)

Predicted band size: 88 kDa

false

Western blot - Anti-BRAT1 antibody [EPR13753-43] - C-terminal (AB181868)
  • WB

Supplier Data

Western blot - Anti-BRAT1 antibody [EPR13753-43] - C-terminal (AB181868)

All lanes:

Western blot - Anti-BRAT1 antibody [EPR13753-43] - C-terminal (ab181868) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 88 kDa

false

  • Carrier free

    Anti-BRAT1 antibody [EPR13753-43] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13753-43

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in DNA damage response; activates kinases ATM, SMC1A and PRKDC by modulating their phosphorylation status following ionizing radiation (IR) stress (PubMed : 16452482, PubMed : 22977523). Plays a role in regulating mitochondrial function and cell proliferation (PubMed : 25070371). Required for protein stability of MTOR and MTOR-related proteins, and cell cycle progress by growth factors (PubMed : 25657994).
See full target information BRAT1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 23:575 PubMed40830790

2025

Engineered MEVs for photoreceptor-targeted delivery of USP25 to alleviate diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yaoxiang Sun,Shenyuan Chen,Xiaoyuan Qi,Yuntong Sun,Zhengmei Jiang,Jie Chang,Yongjun Ma,Jin Huang,Benshuai You,Fengtian Sun
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com