AB250526

Anti-BRAT1 antibody [EPR13753] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal BRAT1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

4 Images

Flow Cytometry (Intracellular) - Anti-BRAT1 antibody [EPR13753] - BSA and Azide free (AB250526)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-BRAT1 antibody [EPR13753] - BSA and Azide free (AB250526)

This data was developed using ab181855, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling BRAT1 with ab181855 at 1/10 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control Rabbit IgG.

Immunocytochemistry/ Immunofluorescence - Anti-BRAT1 antibody [EPR13753] - BSA and Azide free (AB250526)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BRAT1 antibody [EPR13753] - BSA and Azide free (AB250526)

This data was developed using ab181855, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling BRAT1 with ab181855 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG(Alexa Fluor®488) at 1/200 dilution, Conterstained with Dapi blue.

Supplier Data

Immunoprecipitation - Anti-BRAT1 antibody [EPR13753] - BSA and Azide free (AB250526)

This data was developed using ab181855, the same antibody clone in a different buffer formulation.

Western blot analysis of BRAT1 in immunoprecipitation pellets from Human Jurkat lysate. ab181855 used at 1/30 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-BRAT1 antibody [EPR13753] - C-terminal (<a href='/en-us/products/primary-antibodies/brat1-antibody-epr13753-c-terminal-ab181855'>ab181855</a>)

Predicted band size: 88 kDa

Observed band size: 75 kDa

false

Supplier Data

Western blot - Anti-BRAT1 antibody [EPR13753] - BSA and Azide free (AB250526)

This data was developed using ab181855, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-BRAT1 antibody [EPR13753] - C-terminal (<a href='/en-us/products/primary-antibodies/brat1-antibody-epr13753-c-terminal-ab181855'>ab181855</a>) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 88 kDa

Observed band size: 75 kDa

false

Unconjugated

Anti-BRAT1 antibody [EPR13753] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13753

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab250526 is the carrier-free version of ab181855.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of a multiprotein complex required for the assembly of the RNA endonuclease module of the integrator complex (PubMed : 39032489, PubMed : 39032490). Associates with INTS9 and INTS11 in the cytoplasm and blocks the active site of INTS11 to inhibit the endonuclease activity of INTS11 before formation of the full integrator complex (PubMed : 39032489, PubMed : 39032490). Following dissociation of WDR73 of the complex, BRAT1 facilitates the nuclear import of the INTS9-INTS11 heterodimer (PubMed : 39032489). In the nucleus, INTS4 is integrated to the INTS9-INTS11 heterodimer and BRAT1 is released from the mature RNA endonuclease module by inositol hexakisphosphate (InsP6) (PubMed : 39032489). BRAT1 is also involved in DNA damage response; activates kinases ATM, SMC1A and PRKDC by modulating their phosphorylation status following ionizing radiation (IR) stress (PubMed : 16452482, PubMed : 22977523). Plays a role in regulating mitochondrial function and cell proliferation (PubMed : 25070371). Required for protein stability of MTOR and MTOR-related proteins, and cell cycle progress by growth factors (PubMed : 25657994).

See full target information BRAT1

Alternative Names

BAAT1, C7orf27, BRAT1, Integrator complex assembly factor BRAT1, BRCA1-associated ATM activator 1, BRCA1-associated protein required for ATM activation protein 1

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Publications (1)

Recent publications for all applications. Explore the full listand refine your search

Scientific reports 14:15564 PubMed38971897

2024

LILRB4 knockdown inhibits aortic dissection development by regulating pyroptosis and the JAK2/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianxian Xiong,Jiayuan Ling,Jie Yan,Yanyu Duan,Junjian Yu,Wentong Li,Wenbo Yu,Jianfeng Gao,Dilin Xie,Ziyou Liu,Yongzhi Deng,Yongling Liao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BRAT1 antibody [EPR13753] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-BRAT1 antibody [EPR13753] - BSA and Azide free (AB250526)

Immunocytochemistry/ Immunofluorescence - Anti-BRAT1 antibody [EPR13753] - BSA and Azide free (AB250526)

Immunoprecipitation - Anti-BRAT1 antibody [EPR13753] - BSA and Azide free (AB250526)

Western blot - Anti-BRAT1 antibody [EPR13753] - BSA and Azide free (AB250526)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Alternative Names

Publications (1)

Product promise