AB181855

Anti-BRAT1 antibody [EPR13753] - C-terminal

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal BRAT1 antibody. C-terminal. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

BAAT1, C7orf27, BRAT1, BRCA1-associated ATM activator 1, BRCA1-associated protein required for ATM activation protein 1

4 Images

Flow Cytometry (Intracellular) - Anti-BRAT1 antibody [EPR13753] - C-terminal (AB181855)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-BRAT1 antibody [EPR13753] - C-terminal (AB181855)

Intracellular flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling BRAT1 with ab181855 at 1/10 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control Rabbit IgG.

Immunocytochemistry/ Immunofluorescence - Anti-BRAT1 antibody [EPR13753] - C-terminal (AB181855)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BRAT1 antibody [EPR13753] - C-terminal (AB181855)

Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling BRAT1 with ab181855 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG(Alexa Fluor®488) at 1/200 dilution Conterstained with Dapi blue.

Supplier Data

Western blot - Anti-BRAT1 antibody [EPR13753] - C-terminal (AB181855)

All lanes:

Western blot - Anti-BRAT1 antibody [EPR13753] - C-terminal (ab181855) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 88 kDa

Observed band size: 75 kDa

false

Supplier Data

Immunoprecipitation - Anti-BRAT1 antibody [EPR13753] - C-terminal (AB181855)

Western blot analysis of BRAT1 in immunoprecipitation pellets from Human Jurkat lysate. ab181855 used at 1/30 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000. Blocking buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-BRAT1 antibody [EPR13753] - C-terminal (ab181855)

Predicted band size: 88 kDa

Observed band size: 75 kDa

false

Carrier free

Anti-BRAT1 antibody [EPR13753] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13753

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in DNA damage response; activates kinases ATM, SMC1A and PRKDC by modulating their phosphorylation status following ionizing radiation (IR) stress (PubMed : 16452482, PubMed : 22977523). Plays a role in regulating mitochondrial function and cell proliferation (PubMed : 25070371). Required for protein stability of MTOR and MTOR-related proteins, and cell cycle progress by growth factors (PubMed : 25657994).

See full target information BRAT1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed39230574

2024

Quantitative mapping of proteasome interactomes and substrates using ProteasomeID.

Applications

Unspecified application

Species

Unspecified reactive species

Aleksandar Bartolome,Julia C Heiby,Domenico Di Fraia,Ivonne Heinze,Hannah Knaudt,Ellen Spaeth,Omid Omrani,Alberto Minetti,Maleen Hofmann,Joanna M Kirkpatrick,Therese Dau,Alessandro Ori

Cancer cell international 24:121 PubMed38555451

2024

β-hydroxybutyrate inhibits malignant phenotypes of prostate cancer cells through β-hydroxybutyrylation of indoleacetamide-N-methyltransferase.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Zhang,Yunlong Li

Nature communications 13:5026 PubMed36028512

2022

BRAT1 links Integrator and defective RNA processing with neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Zuzana Cihlarova,Jan Kubovciak,Margarita Sobol,Katerina Krejcikova,Jana Sachova,Michal Kolar,David Stanek,Cyril Barinka,Grace Yoon,Keith W Caldecott,Hana Hanzlikova

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BRAT1 antibody [EPR13753] - C-terminal

Flow Cytometry (Intracellular) - Anti-BRAT1 antibody [EPR13753] - C-terminal (AB181855)

Immunocytochemistry/ Immunofluorescence - Anti-BRAT1 antibody [EPR13753] - C-terminal (AB181855)

Western blot - Anti-BRAT1 antibody [EPR13753] - C-terminal (AB181855)

Immunoprecipitation - Anti-BRAT1 antibody [EPR13753] - C-terminal (AB181855)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

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