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Rabbit Polyclonal BRCA1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human BRCA1 aa 950-1000.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human BRCA1 aa 950-1000. The exact immunogen used to generate this antibody is proprietary information. Database link P38398
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Tested
Rat
Expected
Tested
Chimpanzee
Predicted
Predicted
Gorilla
Predicted
Predicted
Orangutan
Predicted
Predicted
Rhesus monkey
Predicted
Predicted

Tested
Tested

Species

Mouse

Dilution info

1/500.00000 - 1/2000.00000

Notes

-

Species

Human

Dilution info

1/500.00000 - 1/2000.00000

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Chimpanzee, Rhesus monkey, Gorilla, Orangutan

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/50.00000 - 1/200.00000

Notes

-

Species

Rat

Dilution info

1/50.00000 - 1/200.00000

Notes

-

Species

Human

Dilution info

1/50.00000 - 1/200.00000

Notes

-

Predicted
Predicted

Species

Chimpanzee, Rhesus monkey, Gorilla, Orangutan

Dilution info

-

Notes

-

Target data

Function

E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage (PubMed:12890688, PubMed:14976165, PubMed:16818604, PubMed:17525340, PubMed:12887909, PubMed:10500182, PubMed:19261748). It is unclear whether it also mediates the formation of other types of polyubiquitin chains (PubMed:12890688). The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability (PubMed:12890688, PubMed:14976165, PubMed:20351172). Regulates centrosomal microtubule nucleation (PubMed:18056443). Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle (PubMed:10724175, PubMed:12183412, PubMed:11836499, PubMed:19261748). Required for FANCD2 targeting to sites of DNA damage (PubMed:12887909). Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation (PubMed:16326698). Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks (PubMed:19369211). Component of the BRCA1-RBBP8 complex which regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1-mediated ubiquitination of RBBP8 (PubMed:16818604). Acts as a transcriptional activator (PubMed:20160719).

Alternative names

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Rabbit Polyclonal BRCA1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human BRCA1 aa 950-1000.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human BRCA1 aa 950-1000. The exact immunogen used to generate this antibody is proprietary information. Database link P38398
Purification technique

Affinity purification

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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12 product images

  • Western blot - Anti-BRCA1 antibody (ab238983), expandable thumbnail

    Western blot - Anti-BRCA1 antibody (ab238983)

    Blocking and dilution buffer: 3% nonfat dry milk in TBST.

    Exposure time: 1 second.

    All lanes: Western blot - Anti-BRCA1 antibody (AB238983) at 1/1000 dilution

    Lane 1: MCF7 (human breast adenocarcinoma cell line) cell extract at 25 µg

    Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 25 µg

    Lane 3: NIH/3T3 (mouse embryo fibroblast cell line) cell extract at 25 µg

    Lane 4: Mouse lung tissue extract at 25 µg

    Secondary

    All lanes: HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 207 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983)

    Paraffin-embedded human breast cancer tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983)

    Paraffin-embedded rat testis tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983)

    Paraffin-embedded rat ovary tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983)

    Paraffin-embedded rat brain tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983)

    Paraffin-embedded rat pancreas tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983)

    Paraffin-embedded mouse liver tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983)

    Paraffin-embedded mouse testis tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983)

    Paraffin-embedded mouse spinal cord tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983)

    Paraffin-embedded mouse brain tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983)

    Paraffin-embedded mouse pancreas tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.

  • Western blot - Anti-BRCA1 antibody (ab238983), expandable thumbnail

    Western blot - Anti-BRCA1 antibody (ab238983)

    Western blot: Anti-BRCA1 antibody (ab238983) staining at 1/500 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab238983 was shown to bind specifically to BRCA1. A band was observed at 270 kDa in wild-type MCF7 cell lysates with a reduction in signal observed in the BRCA1 heterozygous knockout cell line ab286284 (heterozygous knockout cell lysate ab300209). To generate this image, wild-type and BRCA1 heterozygous knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-BRCA1 antibody (AB238983) at 1/500 dilution

    Lane 1: Wild-type MCF7 cell lysate at 20 µg

    Lane 2: BRCA1 heterozygous knockout MCF7 cell lysate at 20 µg

    Lane 3: HeLa cell lysate at 20 µg

    Lane 4: PC-3 cell lysate at 20 µg

    Observed band size: 270 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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