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BiochemicalsProteins and Peptides
Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
Learn about all product ranges with our product overviews.
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Rabbit Polyclonal BRCA1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human BRCA1 aa 950-1000.
IgG
Rabbit
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
WB | IHC-P | |
---|---|---|
Human | Tested | Tested |
Mouse | Tested | Tested |
Rat | Expected | Tested |
Chimpanzee | Predicted | Predicted |
Gorilla | Predicted | Predicted |
Orangutan | Predicted | Predicted |
Rhesus monkey | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Species Rat | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage (PubMed:12890688, PubMed:14976165, PubMed:16818604, PubMed:17525340, PubMed:12887909, PubMed:10500182, PubMed:19261748). It is unclear whether it also mediates the formation of other types of polyubiquitin chains (PubMed:12890688). The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability (PubMed:12890688, PubMed:14976165, PubMed:20351172). Regulates centrosomal microtubule nucleation (PubMed:18056443). Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle (PubMed:10724175, PubMed:12183412, PubMed:11836499, PubMed:19261748). Required for FANCD2 targeting to sites of DNA damage (PubMed:12887909). Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation (PubMed:16326698). Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks (PubMed:19369211). Component of the BRCA1-RBBP8 complex which regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1-mediated ubiquitination of RBBP8 (PubMed:16818604). Acts as a transcriptional activator (PubMed:20160719).
Breast cancer type 1 susceptibility protein, RING finger protein 53, RING-type E3 ubiquitin transferase BRCA1, BRCA1, RNF53
Rabbit Polyclonal BRCA1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human BRCA1 aa 950-1000.
Breast cancer type 1 susceptibility protein, RING finger protein 53, RING-type E3 ubiquitin transferase BRCA1, BRCA1, RNF53
IgG
Rabbit
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking and dilution buffer: 3% nonfat dry milk in TBST.
Exposure time: 1 second.
All lanes: Western blot - Anti-BRCA1 antibody (AB238983) at 1/1000 dilution
Lane 1: MCF7 (human breast adenocarcinoma cell line) cell extract at 25 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 25 µg
Lane 3: NIH/3T3 (mouse embryo fibroblast cell line) cell extract at 25 µg
Lane 4: Mouse lung tissue extract at 25 µg
All lanes: HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 207 kDa
Paraffin-embedded human breast cancer tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded rat testis tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded rat ovary tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded rat brain tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded rat pancreas tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded mouse liver tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded mouse testis tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded mouse spinal cord tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded mouse brain tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded mouse pancreas tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.
Western blot: Anti-BRCA1 antibody (ab238983) staining at 1/500 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab238983 was shown to bind specifically to BRCA1. A band was observed at 270 kDa in wild-type MCF7 cell lysates with a reduction in signal observed in the BRCA1 heterozygous knockout cell line ab286284 (heterozygous knockout cell lysate ab300209). To generate this image, wild-type and BRCA1 heterozygous knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-BRCA1 antibody (AB238983) at 1/500 dilution
Lane 1: Wild-type MCF7 cell lysate at 20 µg
Lane 2: BRCA1 heterozygous knockout MCF7 cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
Lane 4: PC-3 cell lysate at 20 µg
Observed band size: 270 kDa
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