AB9141

Anti-BRCA1 antibody

Name: Anti-BRCA1 antibody (ab9141) | Abcam
Brand: Abcam Limited
SKU: AB9141
Price: 480 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(6 Publications)

Rabbit Polyclonal BRCA1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human BRCA1.

View Alternative Names

RNF53, BRCA1, Breast cancer type 1 susceptibility protein, RING finger protein 53, RING-type E3 ubiquitin transferase BRCA1

2 Images

Supplier Data

Western blot - Anti-BRCA1 antibody (AB9141)

All lanes:

Western blot - Anti-BRCA1 antibody (ab9141) at 0.04 µg/mL

All lanes:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer) at 50 µg

Predicted band size: 207 kDa

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Exposure time: 30s

Supplier Data

Immunoprecipitation - Anti-BRCA1 antibody (AB9141)

Detection of BRCA1 by immunoprecipitation of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer.

Lane 1 : Anti-BRCA1 antibody (ab9141) used for IP at 6 μg per reaction.

Lane 2 : IgG Control

For blotting immunoprecipitated BRCA1, ab9141 was used at 0.04 μg/mL.

Detection : Chemiluminescence with an exposure time of 75 seconds.

All lanes:

Immunoprecipitation - Anti-BRCA1 antibody (ab9141)

Predicted band size: 207 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human BRCA1. The exact immunogen used to generate this antibody is proprietary information.

P38398

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibodies were affinity purified using thepeptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG.

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage (PubMed : 10500182, PubMed : 12887909, PubMed : 12890688, PubMed : 14976165, PubMed : 16818604, PubMed : 17525340, PubMed : 19261748). It is unclear whether it also mediates the formation of other types of polyubiquitin chains (PubMed : 12890688). The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability (PubMed : 12890688, PubMed : 14976165, PubMed : 20351172). Regulates centrosomal microtubule nucleation (PubMed : 18056443). Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle (PubMed : 10724175, PubMed : 11836499, PubMed : 12183412, PubMed : 19261748). Required for FANCD2 targeting to sites of DNA damage (PubMed : 12887909). Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation (PubMed : 16326698). Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks (PubMed : 19369211). Component of the BRCA1-RBBP8 complex which regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1-mediated ubiquitination of RBBP8 (PubMed : 16818604). Acts as a transcriptional activator (PubMed : 20160719).

See full target information BRCA1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37240365

2023

MicroRNA-155-5p, Reduced by Curcumin-Re-Expressed Hypermethylated , Is a Molecular Biomarker for Cancer Risk in -methylation Carriers.

Applications

Unspecified application

Species

Unspecified reactive species

Nisreen Al-Moghrabi,Maram Al-Showimi,Nujoud Al-Yousef,Lamya AlOtai

American journal of cancer research 10:1140-1155 PubMed32368391

2020

CDK9 inhibitor CDKI-73 is synergetic lethal with PARP inhibitor olaparib in BRCA1 wide-type ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajia Li,Xiuling Zhi,Shuyi Chen,Xiaoqing Shen,Chen Chen,Lei Yuan,Jieyu Guo,Dan Meng,Mo Chen,Liangqing Yao

Oncology reports 43:827-838 PubMed32020216

2020

Curcumin induces re‑expression of BRCA1 and suppression of γ synuclein by modulating DNA promoter methylation in breast cancer cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Nujoud Al-Yousef,Zakia Shinwari,Bushra Al-Shahrani,Maram Al-Showimi,Nisreen Al-Moghrabi

Biochemical and biophysical research communication 503:600-606 PubMed29902451

2018

KIAA0101 inhibition suppresses cell proliferation and cell cycle progression by promoting the interaction between p53 and Sp1 in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Lv,Benhua Su,Yuyang Li,Chong Geng,Na Chen

Oncology letters 15:2855-2862 PubMed29456719

2018

Estrogen receptor-α promoter methylation is a biomarker for outcome prediction of cisplatin resistance in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Junnan Xu,Tao Sun,Xiangyu Guo,Yan Wang,Mingxi Jing

Journal of biochemistry and molecular biology 40:15-21 PubMed17244477

2007

Alternative splicing of breast cancer associated gene BRCA1 from breast cancer cell line.

Applications

Species

Human

Miao Lixia,Cao Zhijian,Shen Chao,Gu Chaojiang,Zheng Congyi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BRCA1 antibody

Western blot - Anti-BRCA1 antibody (AB9141)

Immunoprecipitation - Anti-BRCA1 antibody (AB9141)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

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