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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
Learn about all product ranges with our product overviews.
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Rabbit Polyclonal BRCA2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 18 publications. Immunogen corresponding to Synthetic Peptide within Human BRCA2 aa 450-500.
IgG
Rabbit
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate
Liquid
Polyclonal
IP | WB | |
---|---|---|
Human | Tested | Tested |
Chimpanzee | Predicted | Predicted |
Gorilla | Predicted | Predicted |
Orangutan | Predicted | Predicted |
Rhesus monkey | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and SEM1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability. Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, independently of its known role in homologous recombination (PubMed:24896180).
Breast cancer type 2 susceptibility protein, Fanconi anemia group D1 protein, BRCA2, FACD, FANCD1
Rabbit Polyclonal BRCA2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 18 publications. Immunogen corresponding to Synthetic Peptide within Human BRCA2 aa 450-500.
Breast cancer type 2 susceptibility protein, Fanconi anemia group D1 protein, BRCA2, FACD, FANCD1
IgG
Rabbit
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate
Liquid
Polyclonal
Synthetic Peptide within Human BRCA2 aa 450-500. The exact immunogen used to generate this antibody is proprietary information.Database link P51587
Affinity purification Immunogen
Blue Ice
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
All lanes: Western blot - Anti-BRCA2 antibody (AB123491) at 0.1 µg/mL
Lane 1: 293T whole cell lysate at 50 µg
Lane 2: 293T whole cell lysate at 15 µg
Lane 3: 293T whole cell lysate at 5 µg
Lane 5: HeLa whole cell lysate at 50 µg
Lane 6: HeLa whole cell lysate at 15 µg
Predicted band size: 384 kDa
Exposure time: 30s
Detection of Human BRCA2 by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab123491 at 6 µg/mg lysate.
Subsequent Western blot detection used ab123491 at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.
All lanes: Immunoprecipitation - Anti-BRCA2 antibody (AB123491)
Predicted band size: 384 kDa
All lanes: Western blot - Anti-BRCA2 antibody (AB123491) at 0.1 µg/mL
Lane 1: Whole cell lysate from HeLa at 50 µg/mL
Lane 2: Whole cell lysate from HeLa at 15 µg/mL
Lane 3: Whole cell lysate from 293T at 50 µg/mL
Lane 4: Whole cell lysate from Jurkat at 50 µg/mL
Developed using the ECL technique.
Predicted band size: 384 kDa
Exposure time: 10min
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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