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Rabbit Polyclonal BRCA2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 18 publications. Immunogen corresponding to Synthetic Peptide within Human BRCA2 aa 450-500.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human BRCA2 aa 450-500. The exact immunogen used to generate this antibody is proprietary information. Database link P51587
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Chimpanzee
Predicted
Predicted
Gorilla
Predicted
Predicted
Orangutan
Predicted
Predicted
Rhesus monkey
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

2.00000-10.00000 µg/mg of lysate

Notes

-

Predicted
Predicted

Species

Chimpanzee, Rhesus monkey, Gorilla, Orangutan

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/2000.00000 - 1/10000.00000

Notes

-

Predicted
Predicted

Species

Chimpanzee, Rhesus monkey, Gorilla, Orangutan

Dilution info

-

Notes

-

Target data

Function

Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and SEM1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability. Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, independently of its known role in homologous recombination (PubMed:24896180).

Alternative names

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Rabbit Polyclonal BRCA2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 18 publications. Immunogen corresponding to Synthetic Peptide within Human BRCA2 aa 450-500.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen

Synthetic Peptide within Human BRCA2 aa 450-500. The exact immunogen used to generate this antibody is proprietary information.Database link P51587

Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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3 product images

  • Western blot - Anti-BRCA2 antibody (ab123491), expandable thumbnail

    Western blot - Anti-BRCA2 antibody (ab123491)

    All lanes: Western blot - Anti-BRCA2 antibody (AB123491) at 0.1 µg/mL

    Lane 1: 293T whole cell lysate at 50 µg

    Lane 2: 293T whole cell lysate at 15 µg

    Lane 3: 293T whole cell lysate at 5 µg

    Lane 5: HeLa whole cell lysate at 50 µg

    Lane 6: HeLa whole cell lysate at 15 µg

    Predicted band size: 384 kDa

    Exposure time: 30s

  • Immunoprecipitation - Anti-BRCA2 antibody (ab123491), expandable thumbnail

    Immunoprecipitation - Anti-BRCA2 antibody (ab123491)

    Detection of Human BRCA2 by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab123491 at 6 µg/mg lysate.
    Subsequent Western blot detection used ab123491 at 1 µg/ml.
    Detection: Chemiluminescence with an exposure time of 10 seconds.

    All lanes: Immunoprecipitation - Anti-BRCA2 antibody (AB123491)

    Predicted band size: 384 kDa

  • Western blot - Anti-BRCA2 antibody (ab123491), expandable thumbnail

    Western blot - Anti-BRCA2 antibody (ab123491)

    All lanes: Western blot - Anti-BRCA2 antibody (AB123491) at 0.1 µg/mL

    Lane 1: Whole cell lysate from HeLa at 50 µg/mL

    Lane 2: Whole cell lysate from HeLa at 15 µg/mL

    Lane 3: Whole cell lysate from 293T at 50 µg/mL

    Lane 4: Whole cell lysate from Jurkat at 50 µg/mL

    Developed using the ECL technique.

    Predicted band size: 384 kDa

    Exposure time: 10min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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