AB123491

Anti-BRCA2 antibody

Name: Anti-BRCA2 antibody. Rabbit polyclonal (ab123491) | Abcam
Brand: Abcam Limited
SKU: AB123491
Rating: 4 (3 reviews)

(3 Reviews)

(20 Publications)

Rabbit Polyclonal BRCA2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Human BRCA2 aa 450-500.

View Alternative Names

FACD, FANCD1, BRCA2, Breast cancer type 2 susceptibility protein, Fanconi anemia group D1 protein

3 Images

Unknown

Immunoprecipitation - Anti-BRCA2 antibody (AB123491)

Detection of Human BRCA2 by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab123491 at 6 µg/mg lysate.
Subsequent Western blot detection used ab123491 at 1 µg/ml.
Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-BRCA2 antibody (ab123491)

Predicted band size: 384 kDa

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Supplier Data

Western blot - Anti-BRCA2 antibody (AB123491)

All lanes:

Western blot - Anti-BRCA2 antibody (ab123491) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 5 µg

Lane 5:

HeLa whole cell lysate at 50 µg

Lane 6:

HeLa whole cell lysate at 15 µg

Predicted band size: 384 kDa

false

Exposure time: 30s

Unknown

Western blot - Anti-BRCA2 antibody (AB123491)

All lanes:

Western blot - Anti-BRCA2 antibody (ab123491) at 0.1 µg/mL

Lane 1:

Whole cell lysate from HeLa at 50 µg/mL

Lane 2:

Whole cell lysate from HeLa at 15 µg/mL

Lane 3:

Whole cell lysate from 293T at 50 µg/mL

Lane 4:

Whole cell lysate from Jurkat at 50 µg/mL

Predicted band size: 384 kDa

true

Exposure time: 10min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human BRCA2 aa 450-500. The exact immunogen used to generate this antibody is proprietary information.

P51587

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and SEM1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability. Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, independently of its known role in homologous recombination (PubMed : 24896180).

See full target information BRCA2

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37446144

2023

Selective Killing of BRCA2-Deficient Ovarian Cancer Cells via MRE11 Blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Adel Alblihy,Reem Ali,Mashael Algethami,Alison A Ritchie,Ahmed Shoqafi,Shatha Alqahtani,Katia A Mesquita,Michael S Toss,Paloma Ordóñez-Morán,Jennie N Jeyapalan,Lodewijk Dekker,Martina Salerno,Edgar Hartsuiker,Anna M Grabowska,Emad A Rakha,Nigel P Mongan,Srinivasan Madhusudan

Nucleic acids research 51:7357-7375 PubMed37378420

2023

RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Liu,Liying Wang,Yanan Li,Mengmeng Guo,Jun Hu,Teng Wang,Mengjing Li,Zhuo Yang,Ruoyao Lin,Wei Xu,Yinghong Chen,Mengcheng Luo,Fei Gao,Jia-Yu Chen,Qianwen Sun,Hongbin Liu,Bo Sun,Wei Li

Nature cell biology 24:1461-1474 PubMed36109671

2022

An intercellular transfer of telomeres rescues T cells from senescence and promotes long-term immunological memory.

Applications

Unspecified application

Species

Unspecified reactive species

Alessio Lanna,Bruno Vaz,Clara D'Ambra,Salvatore Valvo,Claudia Vuotto,Valerio Chiurchiù,Oliver Devine,Massimo Sanchez,Giovanna Borsellino,Arne N Akbar,Marco De Bardi,Derek W Gilroy,Michael L Dustin,Brendan Blumer,Michael Karin

FEBS letters 596:2041-2055 PubMed35592921

2022

The BRCA2 and CDKN1A-interacting protein (BCCIP) stabilizes stalled replication forks and prevents degradation of nascent DNA.

Applications

Unspecified application

Species

Unspecified reactive species

Bhawna Singh,Shalini Roy Chowdhury,Mohammad Shoab Mansuri,Saraswathi Jayarajan Pillai,Sonam Mehrotra

Cancers 14: PubMed35053516

2022

CDK5RAP3, a New BRCA2 Partner That Regulates DNA Repair, Is Associated with Breast Cancer Survival.

Applications

Unspecified application

Species

Unspecified reactive species

Jordi Minguillón,María José Ramírez,Llorenç Rovirosa,Pilar Bustamante-Madrid,Cristina Camps-Fajol,Gorka Ruiz de Garibay,Hermela Shimelis,Helena Montanuy,Roser Pujol,Gonzalo Hernandez,Massimo Bogliolo,Pau Castillo,Penny Soucy,Griselda Martrat,Antonio Gómez,Daniel Cuadras,María J García,Javier Gayarre, Cimba,Conxi Lázaro,Javier Benítez,Fergus J Couch,Miquel Angel Pujana,Jordi Surrallés

NPJ breast cancer 7:117 PubMed34504103

2021

Clinical consequences of BRCA2 hypomorphism.

Applications

Unspecified application

Species

Unspecified reactive species

Laia Castells-Roca,Sara Gutiérrez-Enríquez,Sandra Bonache,Massimo Bogliolo,Estela Carrasco,Miriam Aza-Carmona,Gemma Montalban,Núria Muñoz-Subirana,Roser Pujol,Cristina Cruz,Alba Llop-Guevara,María J Ramírez,Cristina Saura,Adriana Lasa,Violeta Serra,Orland Diez,Judith Balmaña,Jordi Surrallés

Molecular cell 81:3128-3144.e7 PubMed34216544

2021

Replication gaps are a key determinant of PARP inhibitor synthetic lethality with BRCA deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Cong,Min Peng,Arne Nedergaard Kousholt,Wei Ting C Lee,Silviana Lee,Sumeet Nayak,John Krais,Pamela S VanderVere-Carozza,Katherine S Pawelczak,Jennifer Calvo,Nicholas J Panzarino,John J Turchi,Neil Johnson,Jos Jonkers,Eli Rothenberg,Sharon B Cantor

Cancer research 80:5344-5354 PubMed33023948

2020

Melphalan and Exportin 1 Inhibitors Exert Synergistic Antitumor Effects in Preclinical Models of Human Multiple Myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Joel G Turner,Yan Cui,Alexis A Bauer,Jana L Dawson,Juan A Gomez,Jongphil Kim,Christopher L Cubitt,Taiga Nishihori,William S Dalton,Daniel M Sullivan

American journal of cancer research 10:2813-2831 PubMed33042619

2020

Novel mutations in intron 11 and overexpression of COX-2 and BIRC3 mediate cellular resistance to PARP inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Hua-Dong Chen,Ne Guo,Shan-Shan Song,Chuan-Huizi Chen,Ze-Hong Miao,Jin-Xue He

Communications biology 3:388 PubMed32681145

2020

Ribosome profiling reveals a functional role for autophagy in mRNA translational control.

Applications

Unspecified application

Species

Unspecified reactive species

Juliet Goldsmith,Timothy Marsh,Saurabh Asthana,Andrew M Leidal,Deepthisri Suresh,Adam Olshen,Jayanta Debnath

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BRCA2 antibody

Immunoprecipitation - Anti-BRCA2 antibody (AB123491)

Western blot - Anti-BRCA2 antibody (AB123491)

Western blot - Anti-BRCA2 antibody (AB123491)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (20)

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