Anti-BRCA2 antibody

• IP

Supplier Data

Immunoprecipitation - Anti-BRCA2 antibody (AB9143)

BRCA2 was immunoprecipitated from HEK293T whole cell lysate (1 mg per IP reaction, 20% loaded) with ab9143 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab9143 at 0.01 μg/mL.

Lane 1 : ab9143 IP in HEK293T whole cell lysate.

Lane 2 : ab9143 IP in HEK293T whole cell lysate.

Lane 3 : Control IgG in HEK293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 30 seconds

All lanes:

Immunoprecipitation - Anti-BRCA2 antibody (ab9143)

Predicted band size: 384 kDa

false

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-BRCA2 antibody (AB9143)

All lanes:

Western blot - Anti-BRCA2 antibody (ab9143) at 0.1 µg/mL

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 2:

OVCAR-3 (human ovary epithelial adenocarcinoma cell line) whole cell lysate at 50 µg

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 50 µg

Lane 4:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 50 µg

Lane 5:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 6:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 7:

U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate at 50 µg

Lane 8:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 50 µg

Predicted band size: 384 kDa

false

Exposure time: 3min