Rabbit Polyclonal BRCA2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human BRCA2.
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Publications
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- Journal of medical genetics :2020Homozygous hypomorphic variant in primary ovarian insufficiency without cancer or Fanconi anaemia trait.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSandrine Caburet,Abdelkader Heddar,Elodie Dardillac,Héléne Creux,Marie Lambert,Sébastien Messiaen,Sophie Tourpin,Gabriel Livera,Bernard S Lopez,Micheline MisrahiPubMed 32482800
- Biomicrofluidics 12:0541082018Microfluidic platforms for rapid screening of cancer affinity reagents by using tissue samples.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLien-Yu Hung et. al.PubMed 30344835
- Cancer research 76:2868-752016Hyperthermia Synergizes with Chemotherapy by Inhibiting PARP1-Dependent DNA Replication Arrest.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLea Schaaf et. al.PubMed 27013194
- Nature 528:422-62015A mechanism for the suppression of homologous recombination in G1 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandre Orthwein et. al.PubMed 26649820
- Scientific reports 4:42582014Inhibition of post-translational N-glycosylation by HRD1 that controls the fate of ABCG5/8 transporter.
- Human molecular genetics 12:2503-102003Direct interaction of the Fanconi anaemia protein FANCG with BRCA2/FANCD1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human BRCA2. The exact immunogen used to generate this antibody is proprietary information. Database link P51587
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Chimpanzee | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Gorilla | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Gorilla | Dilution info - | Notes - |
Associated Products
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Target data
Function
Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and SEM1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability. Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, independently of its known role in homologous recombination (PubMed:24896180).
Alternative names
FACD, FANCD1, BRCA2, Breast cancer type 2 susceptibility protein, Fanconi anemia group D1 protein
Recommended products
Rabbit Polyclonal BRCA2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human BRCA2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human BRCA2. The exact immunogen used to generate this antibody is proprietary information. Database link P51587
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Immunoprecipitation - Anti-BRCA2 antibody (ab9143)
BRCA2 was immunoprecipitated from HEK293T whole cell lysate (1 mg per IP reaction, 20% loaded) with ab9143 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab9143 at 0.01 μg/mL.
Lane 1: ab9143 IP in HEK293T whole cell lysate.
Lane 2: ab9143 IP in HEK293T whole cell lysate.
Lane 3: Control IgG in HEK293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds
All lanes: Immunoprecipitation - Anti-BRCA2 antibody (ab9143)
Predicted band size: 384 kDa
Exposure time: 30s
Western blot - Anti-BRCA2 antibody (ab9143)
All lanes: Western blot - Anti-BRCA2 antibody (ab9143) at 0.1 µg/mL
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2: OVCAR-3 (human ovary epithelial adenocarcinoma cell line) whole cell lysate at 50 µg
Lane 3: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 50 µg
Lane 4: K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 50 µg
Lane 5: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 6: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 7: U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate at 50 µg
Lane 8: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 50 µg
Predicted band size: 384 kDa
Exposure time: 3min
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