Anti-BRCA2 antibody [EPR23442-43]

2 product images

• 

  Western blot - Anti-BRCA2 antibody [EPR23442-43] (ab239375)

  Negative control: Capan-1 (PMID:16251802).
  
  Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
  
  Blocking and diluting buffer: 5% NFDM/TBST

  All lanes: Western blot - Anti-BRCA2 antibody [EPR23442-43] (ab239375)

  Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 100 µg

  Lane 2: Capan-1 (human pancreas adenocarcinoma epithelial cell) whole cell lysate at 100 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

  Predicted band size: 384 kDa

  Exposure time: 180s

• 

  Western blot - Anti-BRCA2 antibody [EPR23442-43] (ab239375)

  Anti-BRCA2 antibody [EPR23442-43] (ab239375) staining at 1/500 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab239375 was shown to bind specifically to BRCA2. A band was observed at 290 kDa in wild-type MCF7 cell lysates with no signal observed at this size in BRCA2 knockout cell line Human BRCA2 knockout (hetero) MCF7 cell line ab286285 (knockout cell lysate ab300210). To generate this image, wild-type and BRCA2 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween$®$ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

  All lanes: Western blot - Anti-BRCA2 antibody [EPR23442-43] (ab239375) at 1/500 dilution

  Lane 1: Wild-type MCF7 cell lysate at 20 µg

  Lane 2: Western blot - Human BRCA2 knockout MCF7 cell lysate (ab300210) at 20 µg

  Lane 3: Jurkat cell lysate at 20 µg

  Lane 4: PC-3 cell lysate at 20 µg

  Lane 5: HT-29 cell lysate at 20 µg

  Secondary

  Lanes 1 - 5: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

  Lanes 1 - 5: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

  Observed band size: 290 kDa