AB108411

Anti-BRCC36 antibody [EPR4366]

RabMAb
Recombinant
KO Validated
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal BRCC36 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 5 publications.

View Alternative Names

BRCC36, C6.1A, CXorf53, BRCC3, Lys-63-specific deubiquitinase BRCC36, BRCA1-A complex subunit BRCC36, BRCA1/BRCA2-containing complex subunit 3, BRCA1/BRCA2-containing complex subunit 36, BRISC complex subunit BRCC36

3 Images

Flow Cytometry (Intracellular) - Anti-BRCC36 antibody [EPR4366] (AB108411)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-BRCC36 antibody [EPR4366] (AB108411)

Intracellular flow cytometric analysis of BRCC36 in permeabilized HeLa cells, using ab108411 at a 1/100 dilution (red) or a Rabbit IgG (negative) (green).

Unknown

Western blot - Anti-BRCC36 antibody [EPR4366] (AB108411)

All lanes:

Western blot - Anti-BRCC36 antibody [EPR4366] (ab108411) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

SKBR-3 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Predicted band size: 36 kDa

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Lab

Western blot - Anti-BRCC36 antibody [EPR4366] (AB108411)

Lane 1 : Wild type HAP1 whole cell lysate (40 μg)
Lane 2 : Empty Lane
Lane 3 : BRCC3 knockout HAP1 whole cell lysate (40 μg)
Lane 4 : MCF7 whole cell lysate (40 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab108411 observed at 36 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab108411 was shown to recognize empty when empty knockout samples were used, along with additional cross-reactive bands. Wild-type and empty knockout samples were subjected to SDS-PAGE. ab108411 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-BRCC36 antibody [EPR4366] (ab108411)

Predicted band size: 36 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4366

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains (PubMed : 19214193, PubMed : 20656690, PubMed : 24075985, PubMed : 26344097). Does not have activity toward 'Lys-48'-linked polyubiquitin chains (PubMed : 19214193, PubMed : 20656690, PubMed : 24075985, PubMed : 26344097). Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs) (PubMed : 14636569, PubMed : 16707425, PubMed : 17525341, PubMed : 19202061, PubMed : 19261746, PubMed : 19261748, PubMed : 19261749). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs) (PubMed : 20656690). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates (PubMed : 20656690, PubMed : 24075985, PubMed : 26195665, PubMed : 26344097). Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex (PubMed : 19214193). The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1 (PubMed : 26195665). Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression (PubMed : 24075985, PubMed : 26344097). Acts as a regulator of the NLRP3 inflammasome by mediating deubiquitination of NLRP3, leading to NLRP3 inflammasome assembly (By similarity). Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (PubMed : 24075985). Deubiquitinates HDAC1 and PWWP2B leading to their stabilization (By similarity).

See full target information BRCC3

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 221: PubMed35938958

2022

Autologous K63 deubiquitylation within the BRCA1-A complex licenses DNA damage recognition.

Applications

Unspecified application

Species

Unspecified reactive species

Qinqin Jiang,Martina Foglizzo,Yaroslav I Morozov,Xuejiao Yang,Arindam Datta,Lei Tian,Vaughn Thada,Weihua Li,Elton Zeqiraj,Roger A Greenberg

EMBO reports 22:e52190 PubMed34309165

2021

Homologous recombination is reduced in female embryonic stem cells by two active X chromosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Yuka Tamura,Tatsuya Ohhata,Hiroyuki Niida,Satoshi Sakai,Chiharu Uchida,Kazuma Masumoto,Fuminori Katou,Anton Wutz,Masatoshi Kitagawa

Frontiers in immunology 10:2783 PubMed31866999

2019

Vitamin D Receptor Inhibits NLRP3 Activation by Impeding Its BRCC3-Mediated Deubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Zebing Rao,Xin Chen,Junxian Wu,Mengjun Xiao,Jing Zhang,Binghao Wang,Lei Fang,Hongjie Zhang,Xiaoming Wang,Shuo Yang,Yunzi Chen

Leukemia 34:404-415 PubMed31576005

2019

Functional characterization of BRCC3 mutations in acute myeloid leukemia with t(8;21)(q22;q22.1).

Applications

Unspecified application

Species

Unspecified reactive species

Tatjana Meyer,Nikolaus Jahn,Stefanie Lindner,Linda Röhner,Anna Dolnik,Daniela Weber,Annika Scheffold,Simon Köpff,Peter Paschka,Verena I Gaidzik,Dirk Heckl,Sebastian Wiese,Benjamin L Ebert,Hartmut Döhner,Lars Bullinger,Konstanze Döhner,Jan Krönke

Molecular cell 68:185-197.e6 PubMed28943315

2017

NLRP3 Phosphorylation Is an Essential Priming Event for Inflammasome Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Song,Zhao-Shan Liu,Wen Xue,Zhao-Fang Bai,Qian-Yi Wang,Jiang Dai,Xin Liu,Yi-Jiao Huang,Hong Cai,Xiao-Yan Zhan,Qiu-Ying Han,Hongxia Wang,Yuan Chen,Hui-Yan Li,Ai-Ling Li,Xue-Min Zhang,Tao Zhou,Tao Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BRCC36 antibody [EPR4366]

Flow Cytometry (Intracellular) - Anti-BRCC36 antibody [EPR4366] (AB108411)

Western blot - Anti-BRCC36 antibody [EPR4366] (AB108411)

Western blot - Anti-BRCC36 antibody [EPR4366] (AB108411)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (5)

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