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AB264293

Anti-BRD1/BRL antibody

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Rabbit Polyclonal BRD1/BRL antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human BRD1 aa 750-800.

View Alternative Names

BRL, BRPF2, BRD1, Bromodomain-containing protein 1, BR140-like protein, Bromodomain and PHD finger-containing protein 2

2 Images
Immunoprecipitation - Anti-BRD1/BRL antibody (AB264293)
  • IP

Supplier Data

Immunoprecipitation - Anti-BRD1/BRL antibody (AB264293)

BRD1/BRL was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear extract with ab264293 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab264293 at 0.4 μg/ml.

Lane 1 &2 : ab264293 IP in HeLa nuclear extract.
Lane 3 : Control IgG.

Exposure time : 30 secs.

All lanes:

Immunoprecipitation - Anti-BRD1/BRL antibody (ab264293)

Predicted band size: 120 kDa

false

Western blot - Anti-BRD1/BRL antibody (AB264293)
  • WB

Supplier Data

Western blot - Anti-BRD1/BRL antibody (AB264293)

4-8% SDS-PAGE

All lanes:

Western blot - Anti-BRD1/BRL antibody (ab264293) at 0.1 µg/mL

Lane 1:

HeLa nuclear extract at 50 µg

Lane 2:

HeLa nuclear extract at 15 µg

Lane 3:

HeLa nuclear extract at 5 µg

Secondary

All lanes:

Goat anti-Rabbit Light Chain HRP Conjugate

Predicted band size: 120 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human BRD1 aa 750-800. The exact immunogen used to generate this antibody is proprietary information.

O95696

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Scaffold subunit of various histone acetyltransferase (HAT) complexes, such as the MOZ/MORF and HBO1 complexes, that acts as a regulator of hematopoiesis (PubMed : 16387653, PubMed : 21753189, PubMed : 21880731). Plays a key role in HBO1 complex by directing KAT7/HBO1 specificity towards histone H3 'Lys-14' acetylation (H3K14ac), thereby promoting erythroid differentiation (PubMed : 21753189).
See full target information BRD1

Product promise

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