Rabbit Polyclonal BRD1/BRL antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.
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Publications
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- Nature communications 10:19152019A chemical toolbox for the study of bromodomains and epigenetic signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQin Wu et. al.PubMed 31015424
- Genes & development 31:1988-20022017Bivalent complexes of PRC1 with orthologs of BRD4 and MOZ/MORF target developmental genes in .Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyuckjoon Kang et. al.PubMed 29070704
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Chicken | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Scaffold subunit of various histone acetyltransferase (HAT) complexes, such as the MOZ/MORF and HBO1 complexes, that acts as a regulator of hematopoiesis (PubMed:16387653, PubMed:21753189, PubMed:21880731). Plays a key role in HBO1 complex by directing KAT7/HBO1 specificity towards histone H3 'Lys-14' acetylation (H3K14ac), thereby promoting erythroid differentiation (PubMed:21753189).
Alternative names
BRL, BRPF2, BRD1, Bromodomain-containing protein 1, BR140-like protein, Bromodomain and PHD finger-containing protein 2
Recommended products
Rabbit Polyclonal BRD1/BRL antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
This image is a courtesy of Antibody Solutions LtdWestern blot - Anti-BRD1/BRL antibody (ab71877)
GC-1 - mouse spermatogonia cell line
All lanes: Western blot - Anti-BRD1/BRL antibody (ab71877) at 1 µg/mL
Lane 1: Lysates prepared from Mouse testis tissue. at 20 µg
Lane 2: Lysates prepared from Mouse Lung tissue. at 20 µg
Lane 3: Lysates prepared from Mouse Kidney tissue. at 20 µg
Lane 4: Lysates prepared from GC-1 cell line. at 20 µg
SecondaryAll lanes: HRP-conjugated pig polyclonal to rabbit IgG at 1/5000 dilution
Performed under reducing conditions.
Observed band size: 150 kDa, 34 kDa, 92 kDa
Exposure time: 7min
Western blot - Anti-BRD1/BRL antibody (ab71877)
All lanes: Western blot - Anti-BRD1/BRL antibody (ab71877) at 1 µg/mL
Lane 1: TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 2: Western blot - Raji whole cell lysate (Raji whole cell lysate ab30124) at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Observed band size: 150 kDa
Immunocytochemistry/ Immunofluorescence - Anti-BRD1/BRL antibody (ab71877)
ICC/IF image of ab71877 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71877, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293 and HepG2 cells at 1µg/ml.
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