Anti-BRD1/BRL antibody [EPR12960]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] (AB181060)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling BRD1/BRL with ab181060 at 1/200 dilution (3.46 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human testis (PMID : 10602503). The section was incubated with ab181060 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] (AB181060)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling BRD1/BRL with ab181060 at 1/200 dilution (3.46 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human lung carcinoma. The section was incubated with ab181060 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

• IP

Lab

Immunoprecipitation - Anti-BRD1/BRL antibody [EPR12960] (AB181060)

ab181060 at 1/30 dilution (2μg in 0.35mg lysates) immunoprecipitating BRD1/BRL in K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate.

Lane 1 (input) : K-562 whole cell lysate (10μg)

Lane 2 (+) : ab181060 + K-562 whole cell lysate

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab181060 in K-562 whole cell lysate

For western blotting, ab181060 at 1/1000 dilution (0.691 μg/mL) followed by VeriBlot for IP (HRP) (ab131366) was used for detection (1/5000).

Blocking and Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

Predicted band size: 120 kDa

Observed band size: 120 kDa

false

Exposure time: 111s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] (AB181060)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling BRD1/BRL with ab181060 at 1/200 dilution (3.46 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat testis (PMID : 10602503). The section was incubated with ab181060 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] (AB181060)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling BRD1/BRL with ab181060 at 1/200 dilution (3.46 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse testis (PMID : 10602503). The section was incubated with ab181060 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

• IP

Lab

Immunoprecipitation - Anti-BRD1/BRL antibody [EPR12960] (AB181060)

ab181060 at 1/30 dilution (2μg in 0.35mg lysates) immunoprecipitating BRD1/BRL in mouse testis tissue lysate.

Lane 1 (input) : Mouse testis tissue lysate (10μg)

Lane 2 (+) : ab181060 + Mouse testis tissue lysate

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab181060 in mouse testis tissue lysate

For western blotting, ab181060 at 1/1000 dilution (0.691 μg/mL) followed by VeriBlot for IP (HRP) (ab131366) was used for detection (1/5000).

Blocking and Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

Predicted band size: 120 kDa

Observed band size: 120 kDa

false

Exposure time: 111s

• WB

Supplier Data

Western blot - Anti-BRD1/BRL antibody [EPR12960] (AB181060)

Blocking/ Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-BRD1/BRL antibody [EPR12960] (ab181060) at 1/2000 dilution

Lane 1:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Lane 2:

Raji cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 120 kDa

false