AB238167

Anti-BRD2 (phospho S37) antibody [EPR22114] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal BRD2 phospho S37 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 1 publication.

View Alternative Names

KIAA9001, RING3, BRD2, Bromodomain-containing protein 2, O27.1.1

2 Images

Supplier Data

Western blot - Anti-BRD2 (phospho S37) antibody [EPR22114] - BSA and Azide free (AB238167)

Blocking/diluting buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223255).

All lanes:

Western blot - Anti-BRD2 (phospho S37) antibody [EPR22114] - BSA and Azide free (ab238167) at 1/5000 dilution

Lane 1:

Untreated HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 10 µg

Lane 2:

HEK-293 treated with 100 nM Calyculin A for 30 minutes, whole cell lysate at 10 µg

Lane 3:

HEK-293 treated with 100 nM Calyculin A for 30 minutes, whole cell lysate then treated with alkaline phosphatase for 1 hour at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 88 kDa

false

Exposure time: 92s

Unknown

Dot Blot - Anti-BRD2 (phospho S37) antibody [EPR22114] - BSA and Azide free (AB238167)

Dot blot analysis of BRD2 (phospho S37) labeled with ab223255 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody was used at 1/1000,000 dilution.

Lane 1 : BRD2 (phospho S37) peptide (aa 31-41).

Lane 2 : BRD2 (phospho S37) peptide (aa 34-44).

Lane 3 : BRD2 non-phospho peptide (aa 31-41).

Exposure time : 10 seconds.

Blocking/Dilution buffer and concentration : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223255).

Unconjugated

Anti-BRD2 (phospho S37) antibody [EPR22114]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22114

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

Product details

ab238167 is the carrier-free version of ab223255.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Chromatin reader protein that specifically recognizes and binds histone H4 acetylated at 'Lys-5' and 'Lys-12' (H4K5ac and H4K12ac, respectively), thereby controlling gene expression and remodeling chromatin structures (PubMed : 17148447, PubMed : 17848202, PubMed : 18406326, PubMed : 20048151, PubMed : 20709061, PubMed : 20871596). Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation (PubMed : 28262505). Plays a key role in genome compartmentalization via its association with CTCF and cohesin : recruited to chromatin by CTCF and promotes formation of topologically associating domains (TADs) via its ability to bind acetylated histones, contributing to CTCF boundary formation and enhancer insulation (PubMed : 35410381). Also recognizes and binds acetylated non-histone proteins, such as STAT3 (PubMed : 28262505). Involved in inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 : recognizes and binds STAT3 acetylated at 'Lys-87', promoting STAT3 recruitment to chromatin (PubMed : 28262505). In addition to acetylated lysines, also recognizes and binds lysine residues on histones that are both methylated and acetylated on the same side chain to form N6-acetyl-N6-methyllysine (Kacme), an epigenetic mark of active chromatin associated with increased transcriptional initiation (PubMed : 37731000). Specifically binds histone H4 acetyl-methylated at 'Lys-5' and 'Lys-12' (H4K5acme and H4K12acme, respectively) (PubMed : 37731000).

See full target information BRD2 phospho S37

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1162669 PubMed37207208

2023

Naproxen chemoprevention induces proliferation of cytotoxic lymphocytes in Lynch Syndrome colorectal mucosa.

Applications

Unspecified application

Species

Unspecified reactive species

Charles M Bowen,Nan Deng,Laura Reyes-Uribe,Edwin Roger Parra,Pedro Rocha,Luisa M Solis,Ignacio I Wistuba,Valerie O Sepeda,Lana Vornik,Marjorie Perloff,Eva Szabo,Asad Umar,Krishna M Sinha,Powel H Brown,Eduardo Vilar

View all publications

style

left-column

style

left-column

style

right-column

Alternative Products

Primary Antibodies

AB245436

Anti-BRD2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD2 antibody (AB245436)