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Mouse Monoclonal BRD3 antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 23 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BRD3.


Images

Immunoprecipitation - Anti-BRD3 antibody [2088C3a] (AB50818), expandable thumbnail
  • Western blot - Anti-BRD3 antibody [2088C3a] (AB50818), expandable thumbnail
  • Western blot - Anti-BRD3 antibody [2088C3a] (AB50818), expandable thumbnail
  • Flow Cytometry - Anti-BRD3 antibody [2088C3a] (AB50818), expandable thumbnail
  • Western blot - Anti-BRD3 antibody [2088C3a] (AB50818), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human BRD3. The exact immunogen used to generate this antibody is proprietary information. Database link Q15059

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IF
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Expected
Rat
Expected
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1.00000-5.00000 µg/mg of lysate
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
0.5 µg/mL
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Species
Rat
Dilution info
1/50
Notes

-

Species
Human
Dilution info
1/50
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Chromatin reader that recognizes and binds acetylated histones, thereby controlling gene expression and remodeling chromatin structures (PubMed:18406326, PubMed:22464331, PubMed:27105114, PubMed:32895492). Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation (PubMed:29567837, PubMed:32895492). In vitro, binds acetylated lysine residues on the N-terminus of histone H2A, H2B, H3 and H4 (PubMed:18406326). Involved in endoderm differentiation via its association with long non-coding RNA (lncRNA) DIGIT: BRD3 undergoes liquid-liquid phase separation upon binding to lncRNA DIGIT, promoting binding to histone H3 acetylated at 'Lys-18' (H3K18ac) to induce endoderm gene expression (PubMed:32895492). Also binds non-histones acetylated proteins, such as GATA1 and GATA2: regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity).

Alternative names

KIAA0043, RING3L, BRD3, Bromodomain-containing protein 3, RING3-like protein

Recommended products

Mouse Monoclonal BRD3 antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 23 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BRD3.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human BRD3. The exact immunogen used to generate this antibody is proprietary information. Database link Q15059
Clone number
2088C3a
Purification technique
Affinity purification Protein G
Concentration
Loading...
Purification notes

Purified from TCS

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Notes

This product was changed from ascites to tissue culture supernatant on 9th August 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Product promise

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8 product images

  • Immunoprecipitation - Anti-BRD3 antibody [2088C3a] (ab50818), expandable thumbnail

    Immunoprecipitation - Anti-BRD3 antibody [2088C3a] (ab50818)

    Immunoprecipitation of BRD3 from RIPA lysate of HeLa cells (500µg) using 5µg ab50818. Visualized by western blot using 1/50 ab50518 as primary and anti-mouse IgG secondary (1:2500). Predicted band size 80 KDa.

    All lanes: Immunoprecipitation - Anti-BRD3 antibody [2088C3a] (ab50818)

    Predicted band size: 80 kDa

  • Western blot - Anti-BRD3 antibody [2088C3a] (ab50818), expandable thumbnail

    Western blot - Anti-BRD3 antibody [2088C3a] (ab50818)

    Lanes 1-4: Merged signal (red and green). Green - ab50818 observed at 100 kDa. Red - loading control Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 observed at 36 kDa.

    ab50818 Anti-BRD3 antibody [2088C3a] was shown to specifically react with BRD3 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human BRD3 knockout HEK-293T cell line ab266793 (knockout cell lysate Human BRD3 knockout HEK-293T cell lysate ab258335) was used. Wild-type and BRD3 knockout samples were subjected to SDS-PAGE. ab50818 and Anti-GAPDH antibody[EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-BRD3 antibody [2088C3a] (ab50818) at 1/500 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: BRD3 knockout HEK293T cell lysate at 20 µg

    Lane 2: Western blot - Human BRD3 knockout HEK-293T cell line (Human BRD3 knockout HEK-293T cell line ab266793)

    Lane 3: Jurkat cell lysate at 20 µg

    Lane 4: HeLa cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/10000 dilution

    Predicted band size: 80 kDa

    Observed band size: 100 kDa

  • Western blot - Anti-BRD3 antibody [2088C3a] (ab50818), expandable thumbnail

    Western blot - Anti-BRD3 antibody [2088C3a] (ab50818)

    All lanes: Western blot - Anti-BRD3 antibody [2088C3a] (ab50818) at 1/200 dilution

    All lanes: F2408 whole cell lysate at 50 µg

    Secondary

    All lanes: Anti Mouse IgG antibody at 1/2500 dilution

    Predicted band size: 80 kDa

    Observed band size: 100 kDa

  • Flow Cytometry - Anti-BRD3 antibody [2088C3a] (ab50818), expandable thumbnail

    Flow Cytometry - Anti-BRD3 antibody [2088C3a] (ab50818)

    Flow Cytometric analysis using ab50818 (0.5ug). Secondary antibody used: AlexaFluor®488 Goat Anti-mouse IgG (0.25 µg)

  • Western blot - Anti-BRD3 antibody [2088C3a] (ab50818), expandable thumbnail

    Western blot - Anti-BRD3 antibody [2088C3a] (ab50818)

    All lanes: Western blot - Anti-BRD3 antibody [2088C3a] (ab50818) at 1/50 dilution

    All lanes: HeLa whole cell lysate at 50 µg

    Secondary

    All lanes: Anti-Mouse IgG antibody at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 80 kDa

    Observed band size: 90 kDa

  • Western blot - Anti-BRD3 antibody [2088C3a] (ab50818), expandable thumbnail

    Western blot - Anti-BRD3 antibody [2088C3a] (ab50818)

    All lanes: Western blot - Anti-BRD3 antibody [2088C3a] (ab50818) at 1/50 dilution

    All lanes: NIH3T3 whole cell lysate at 50 µg

    Secondary

    All lanes: Anti-Mouse IgG antibody at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 80 kDa

    Observed band size: 90 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-BRD3 antibody [2088C3a] (ab50818), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-BRD3 antibody [2088C3a] (ab50818)

    Immunoflourescent staining of Hela cells using ab50818 Anti-BRD3 Antibody (1/10 dilution) as primary and AlexaFluor®488 Goat Anti-mouse IgG( 1/200 dilution )

  • Western blot - Anti-BRD3 antibody [2088C3a] (ab50818), expandable thumbnail

    Western blot - Anti-BRD3 antibody [2088C3a] (ab50818)

    Western blot: Anti-BRD3 antibody [2088C3a] (ab50818) staining at 1/200 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab50818 was shown to bind specifically to BRD3. A band was observed at 90-100 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in BRD3 knockout cell line. To generate this image, wild-type and BRD3 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were HRP conjugated Goat anti-Mouse (H+L) and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-BRD3 antibody [2088C3a] (ab50818) at 1/200 dilution

    Lane 1: Wild-type HCT 116 cell lysate at 20 µg

    Lane 2: BRD3 knockout HCT 116 cell lysate at 20 µg

    Lane 3: Wild-type HEK-293T ab255553 cell lysate at 20 µg

    Lane 4: BRD3 knockout HEK-293T ab260529 cell lysate at 20 µg

    Secondary

    Lanes 1 - 4: HRP conjugated Goat anti-Mouse (H+L) at 1/20000 dilution

    Lanes 1 - 4: Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 90-100 kDa

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Product protocols

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