AB264420

Anti-BRD3 antibody [BLR069G]

Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal BRD3 antibody. Suitable for IHC-P, ICC, IP, WB, Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human BRD3 aa 650 to C-terminus.

View Alternative Names

KIAA0043, RING3L, BRD3, Bromodomain-containing protein 3, RING3-like protein

7 Images

Flow Cytometry - Anti-BRD3 antibody [BLR069G] (AB264420)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-BRD3 antibody [BLR069G] (AB264420)

Flow cytometry overlay histogram of 4% formaldehyde fixed K562 cells permeabilized with 90% methanol labeling human BRD3 with ab264420 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] (AB264420)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] (AB264420)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling BRD3 with ab264420 at 1/200 dilution. Detection : DAB. Secondary : DyLight^® 594-conjugated goat anti-rabbit IgG. Counterstain : DAPI (blue).

Supplier Data

Immunocytochemistry - Anti-BRD3 antibody [BLR069G] (AB264420)

Immunocytochemical analysis of Formalin-Fixed Paraffin-Embedded OVCAR-3 cells labeling human BRD3 with ab264420 followed by a HRP-conjugated goat anti-rabbit IgG as secondary. Substrate : DAB. Nuclear staining on OVCAR-3 cells is observed. Counter stained with hematoxylin. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes is recommended.

Supplier Data

Western blot - Anti-BRD3 antibody [BLR069G] (AB264420)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-BRD3 antibody [BLR069G] (ab264420) at 1/1000 dilution

Lane 1:

HEK-293T whole cell lysate at 50 µg

Lane 2:

RKO whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

LNcaP whole cell lysate at 50 µg

Lane 5:

HeLa whole cell lysate at 50 µg

Lane 6:

MCF7 whole cell lysate at 50 µg

Lane 7:

K562 whole cell lysate at 50 µg

Lane 8:

HepG2 whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 80 kDa

true

Exposure time: 10s

Supplier Data

Immunocytochemistry - Anti-BRD3 antibody [BLR069G] (AB264420)

Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-3 cells labeling BRD3 with ab264420 at 1/250 dilution. Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] (AB264420)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling BRD3 with ab264420 at 1/250 dilution. Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Supplier Data

Immunoprecipitation - Anti-BRD3 antibody [BLR069G] (AB264420)

BRD3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264420 at 20 μl/mg lysate. Western blot was performed from the immunoprecipitates using ab264420 at 1/1000 dilution.

Lane 1 : ab264420 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 1 second.

Lysates prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-BRD3 antibody [BLR069G] (ab264420)

Predicted band size: 80 kDa

false

Unconjugated

Anti-BRD3 antibody [BLR069G] - BSA free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR069G

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, IP, IHC-P, WB, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human BRD3 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15059

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use 20 μl/mg lysate.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µL for 10^6 Cells", "FlowCyt-species-notes": "" } } }

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Product details

This product is sold under License from Bethyl Laboratories, Inc.

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Properties and storage information

Form

Liquid

Purification notes

Purified from cell culture supernatant.

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 99% Borate buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Chromatin reader that recognizes and binds acetylated histones, thereby controlling gene expression and remodeling chromatin structures (PubMed : 18406326, PubMed : 22464331, PubMed : 27105114, PubMed : 32895492). Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation (PubMed : 29567837, PubMed : 32895492). In vitro, binds acetylated lysine residues on the N-terminus of histone H2A, H2B, H3 and H4 (PubMed : 18406326). Involved in endoderm differentiation via its association with long non-coding RNA (lncRNA) DIGIT : BRD3 undergoes liquid-liquid phase separation upon binding to lncRNA DIGIT, promoting binding to histone H3 acetylated at 'Lys-18' (H3K18ac) to induce endoderm gene expression (PubMed : 32895492). Also binds non-histones acetylated proteins, such as GATA1 and GATA2 : regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity).

See full target information BRD3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 11:900 PubMed33093476

2020

BET degrader inhibits tumor progression and stem-like cell growth via Wnt/β-catenin signaling repression in glioma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Tian,Tongqi Guo,Wei Zhen,Jianjun Zou,Fuyong Li

Frontiers in oncology 9:1471 PubMed31993368

2020

Targeting BET Proteins With a PROTAC Molecule Elicits Potent Anticancer Activity in HCC Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Huapeng Zhang,Gongquan Li,Yi Zhang,Jihua Shi,Bing Yan,Hongwei Tang,Sanyang Chen,Jiakai Zhang,Peihao Wen,Zhihui Wang,Chun Pang,Jie Li,Wenzhi Guo,Shuijun Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BRD3 antibody [BLR069G]

Flow Cytometry - Anti-BRD3 antibody [BLR069G] (AB264420)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] (AB264420)

Immunocytochemistry - Anti-BRD3 antibody [BLR069G] (AB264420)

Western blot - Anti-BRD3 antibody [BLR069G] (AB264420)

Immunocytochemistry - Anti-BRD3 antibody [BLR069G] (AB264420)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] (AB264420)

Immunoprecipitation - Anti-BRD3 antibody [BLR069G] (AB264420)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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