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Rabbit Recombinant Monoclonal BRD3 antibody. Suitable for IHC-P, ICC, IP, WB, Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human BRD3 aa 650 to C-terminus.

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Images

Immunocytochemistry - Anti-BRD3 antibody [BLR069G] (AB264420), expandable thumbnail
  • Western blot - Anti-BRD3 antibody [BLR069G] (AB264420), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] (AB264420), expandable thumbnail
  • Immunoprecipitation - Anti-BRD3 antibody [BLR069G] (AB264420), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] (AB264420), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human BRD3 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q15059

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICCIPWBFlow Cyt
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/100 - 1/500
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/100 - 1/500
Notes

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Tested
Tested

Species
Human
Dilution info
-
Notes

Use 20 μl/mg lysate.

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µL for 106 Cells
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Chromatin reader that recognizes and binds acetylated histones, thereby controlling gene expression and remodeling chromatin structures (PubMed:18406326, PubMed:22464331, PubMed:27105114, PubMed:32895492). Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation (PubMed:29567837, PubMed:32895492). In vitro, binds acetylated lysine residues on the N-terminus of histone H2A, H2B, H3 and H4 (PubMed:18406326). Involved in endoderm differentiation via its association with long non-coding RNA (lncRNA) DIGIT: BRD3 undergoes liquid-liquid phase separation upon binding to lncRNA DIGIT, promoting binding to histone H3 acetylated at 'Lys-18' (H3K18ac) to induce endoderm gene expression (PubMed:32895492). Also binds non-histones acetylated proteins, such as GATA1 and GATA2: regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity).

Alternative names

KIAA0043, RING3L, BRD3, Bromodomain-containing protein 3, RING3-like protein

Recommended products

Rabbit Recombinant Monoclonal BRD3 antibody. Suitable for IHC-P, ICC, IP, WB, Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human BRD3 aa 650 to C-terminus.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human BRD3 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q15059
Clone number
BLR069G
Concentration
Loading...
Purification notes

Purified from cell culture supernatant.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is sold under License from Bethyl Laboratories, Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunocytochemistry - Anti-BRD3 antibody [BLR069G] (ab264420), expandable thumbnail

    Immunocytochemistry - Anti-BRD3 antibody [BLR069G] (ab264420)

    Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-3 cells labeling BRD3 with ab264420 at 1/250 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

  • Western blot - Anti-BRD3 antibody [BLR069G] (ab264420), expandable thumbnail

    Western blot - Anti-BRD3 antibody [BLR069G] (ab264420)

    Lysates prepared using NETN lysis buffer.

    All lanes: Western blot - Anti-BRD3 antibody [BLR069G] (ab264420) at 1/1000 dilution

    Lane 1: HEK-293T whole cell lysate at 50 µg

    Lane 2: RKO whole cell lysate at 50 µg

    Lane 3: Jurkat whole cell lysate at 50 µg

    Lane 4: LNcaP whole cell lysate at 50 µg

    Lane 5: HeLa whole cell lysate at 50 µg

    Lane 6: MCF7 whole cell lysate at 50 µg

    Lane 7: K562 whole cell lysate at 50 µg

    Lane 8: HepG2 whole cell lysate at 50 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 80 kDa

    Exposure time: 10s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] (ab264420), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] (ab264420)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling BRD3 with ab264420 at 1/200 dilution. Detection: DAB. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI (blue).

  • Immunoprecipitation - Anti-BRD3 antibody [BLR069G] (ab264420), expandable thumbnail

    Immunoprecipitation - Anti-BRD3 antibody [BLR069G] (ab264420)

    BRD3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264420 at 20 μl/mg lysate. Western blot was performed from the immunoprecipitates using ab264420 at 1/1000 dilution.

    Lane 1: ab264420 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 1 second.

    Lysates prepared using NETN lysis buffer.

    All lanes: Immunoprecipitation - Anti-BRD3 antibody [BLR069G] (ab264420)

    Predicted band size: 80 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] (ab264420), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] (ab264420)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling BRD3 with ab264420 at 1/250 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

  • Flow Cytometry - Anti-BRD3 antibody [BLR069G] (ab264420), expandable thumbnail

    Flow Cytometry - Anti-BRD3 antibody [BLR069G] (ab264420)

    Flow cytometry overlay histogram of 4% formaldehyde fixed K562 cells permeabilized with 90% methanol labeling human BRD3 with ab264420 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details: Goat Anti-Rabbit IgG H&L (DyLight® 650) (Goat Anti-Rabbit IgG H&L (DyLight® 650) ab96886).

  • Immunocytochemistry - Anti-BRD3 antibody [BLR069G] (ab264420), expandable thumbnail

    Immunocytochemistry - Anti-BRD3 antibody [BLR069G] (ab264420)

    Immunocytochemical analysis of Formalin-Fixed Paraffin-Embedded OVCAR-3 cells labeling human BRD3 with ab264420 followed by a HRP-conjugated goat anti-rabbit IgG as secondary. Substrate: DAB. Nuclear staining on OVCAR-3 cells is observed. Counter stained with hematoxylin. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes is recommended.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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