Anti-BRD3 antibody [BLR069G] - BSA free

• ICC

Supplier Data

Immunocytochemistry - Anti-BRD3 antibody [BLR069G] - BSA free (AB272081)

Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-3 cells labeling BRD3 with ab264420 at 1/250 dilution. Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab264420).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] - BSA free (AB272081)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling BRD3 with ab264420 at 1/250 dilution. Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab264420).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] - BSA free (AB272081)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling BRD3 with ab264420 at 1/200 dilution. Detection : DAB. Secondary : DyLight^® 594-conjugated goat anti-rabbit IgG. Counterstain : DAPI (blue).

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab264420).

• IP

Supplier Data

Immunoprecipitation - Anti-BRD3 antibody [BLR069G] - BSA free (AB272081)

BRD3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264420 at 20 µl/mg lysate. Western blot was performed from the immunoprecipitates using ab264420 at 1/1000 dilution.

Lane 1 : ab264420 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 1 second.

Lysates prepared using NETN lysis buffer.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab264420).

All lanes:

Immunoprecipitation - Anti-BRD3 antibody [BLR069G] (<a href='/en-us/products/primary-antibodies/brd3-antibody-blr069g-ab264420'>ab264420</a>)

Predicted band size: 80 kDa

false

• WB

Supplier Data

Western blot - Anti-BRD3 antibody [BLR069G] - BSA free (AB272081)

Lysates prepared using NETN lysis buffer.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab264420).

All lanes:

Western blot - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081) at 1/1000 dilution

Lane 1:

HEK-293T whole cell lysate at 50 µg

Lane 2:

RKO whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

LNcaP whole cell lysate at 50 µg

Lane 5:

HeLa whole cell lysate at 50 µg

Lane 6:

MCF7 whole cell lysate at 50 µg

Lane 7:

K562 whole cell lysate at 50 µg

Lane 8:

HepG2 whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 80 kDa

true

Exposure time: 10s