Rabbit Recombinant Monoclonal BRD3 antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human BRD3 aa 650 to C-terminus.
Images
![Immunocytochemistry - Anti-BRD3 antibody [BLR069G] - BSA free (AB272081), expandable thumbnail](https://content.abcam.com/products/images/brd3-antibody-blr069g-bsa-free-ab272081--immunocytochemistry-img20966.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-BRD3 antibody [BLR069G] - BSA free (AB272081)")
![Western blot - Anti-BRD3 antibody [BLR069G] - BSA free (AB272081), expandable thumbnail](https://content.abcam.com/products/images/brd3-antibody-blr069g-bsa-free-ab272081--western-blot-img184159.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BRD3 antibody [BLR069G] - BSA free (AB272081)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] - BSA free (AB272081), expandable thumbnail](https://content.abcam.com/products/images/brd3-antibody-blr069g-bsa-free-ab272081--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103514.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] - BSA free (AB272081)")
![Immunoprecipitation - Anti-BRD3 antibody [BLR069G] - BSA free (AB272081), expandable thumbnail](https://content.abcam.com/products/images/brd3-antibody-blr069g-bsa-free-ab272081--immunoprecipitation-img69471.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-BRD3 antibody [BLR069G] - BSA free (AB272081)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] - BSA free (AB272081), expandable thumbnail](https://content.abcam.com/products/images/brd3-antibody-blr069g-bsa-free-ab272081--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110368.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] - BSA free (AB272081)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 8.2
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human BRD3 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q15059
Reactivity data
Select an application| IHC-P | ICC | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Chromatin reader that recognizes and binds acetylated histones, thereby controlling gene expression and remodeling chromatin structures (PubMed:18406326, PubMed:22464331, PubMed:27105114, PubMed:32895492). Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation (PubMed:29567837, PubMed:32895492). In vitro, binds acetylated lysine residues on the N-terminus of histone H2A, H2B, H3 and H4 (PubMed:18406326). Involved in endoderm differentiation via its association with long non-coding RNA (lncRNA) DIGIT: BRD3 undergoes liquid-liquid phase separation upon binding to lncRNA DIGIT, promoting binding to histone H3 acetylated at 'Lys-18' (H3K18ac) to induce endoderm gene expression (PubMed:32895492). Also binds non-histones acetylated proteins, such as GATA1 and GATA2: regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity).
Alternative names
KIAA0043, RING3L, BRD3, Bromodomain-containing protein 3, RING3-like protein
Recommended products
Rabbit Recombinant Monoclonal BRD3 antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human BRD3 aa 650 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 8.2
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human BRD3 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q15059
- Clone number
- BLR069G
- Concentration
- Purification notes
Purified from cell culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab272081 is the BSA-free version of Anti-BRD3 antibody [BLR069G] ab264420.
This product is sold under License from Bethyl Laboratories, Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunocytochemistry - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081), expandable thumbnail](https://content.abcam.com/products/images/brd3-antibody-blr069g-bsa-free-ab272081--immunocytochemistry-img20966.jpg "Immunocytochemistry - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)")
Immunocytochemistry - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)
Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-3 cells labeling BRD3 with Anti-BRD3 antibody [BLR069G] ab264420 at 1/250 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-BRD3 antibody [BLR069G] ab264420).
![Western blot - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081), expandable thumbnail](https://content.abcam.com/products/images/brd3-antibody-blr069g-bsa-free-ab272081--western-blot-img184159.jpg "Western blot - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)")
Western blot - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)
Lysates prepared using NETN lysis buffer.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-BRD3 antibody [BLR069G] ab264420).
All lanes: Western blot - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081) at 1/1000 dilution
Lane 1: HEK-293T whole cell lysate at 50 µg
Lane 2: RKO whole cell lysate at 50 µg
Lane 3: Jurkat whole cell lysate at 50 µg
Lane 4: LNcaP whole cell lysate at 50 µg
Lane 5: HeLa whole cell lysate at 50 µg
Lane 6: MCF7 whole cell lysate at 50 µg
Lane 7: K562 whole cell lysate at 50 µg
Lane 8: HepG2 whole cell lysate at 50 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 80 kDa
Exposure time: 10s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081), expandable thumbnail](https://content.abcam.com/products/images/brd3-antibody-blr069g-bsa-free-ab272081--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103514.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling BRD3 with Anti-BRD3 antibody [BLR069G] ab264420 at 1/200 dilution. Detection: DAB. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-BRD3 antibody [BLR069G] ab264420).
![Immunoprecipitation - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081), expandable thumbnail](https://content.abcam.com/products/images/brd3-antibody-blr069g-bsa-free-ab272081--immunoprecipitation-img69471.jpg "Immunoprecipitation - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)")
Immunoprecipitation - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)
BRD3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with Anti-BRD3 antibody [BLR069G] ab264420 at 20 µl/mg lysate. Western blot was performed from the immunoprecipitates using Anti-BRD3 antibody [BLR069G] ab264420 at 1/1000 dilution.
Lane 1: Anti-BRD3 antibody [BLR069G] ab264420 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with an exposure time of 1 second.
Lysates prepared using NETN lysis buffer.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-BRD3 antibody [BLR069G] ab264420).
All lanes: Immunoprecipitation - Anti-BRD3 antibody [BLR069G] (Anti-BRD3 antibody [BLR069G] ab264420)
Predicted band size: 80 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081), expandable thumbnail](https://content.abcam.com/products/images/brd3-antibody-blr069g-bsa-free-ab272081--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110368.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling BRD3 with Anti-BRD3 antibody [BLR069G] ab264420 at 1/250 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-BRD3 antibody [BLR069G] ab264420).
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