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AB228936

Anti-BRD3 antibody - C-terminal

4

(1 Review)

|

(2 Publications)

Rabbit Polyclonal BRD3 antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BRD3.

View Alternative Names

KIAA0043, RING3L, BRD3, Bromodomain-containing protein 3, RING3-like protein

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-BRD3 antibody - C-terminal (AB228936)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BRD3 antibody - C-terminal (AB228936)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling BRD3 protein at nucleus using ab228936 at a 1/500 dilution (Green).
phalloidin, a cytoskeleton marker, stained by phalloidin antibody at a 1/200 dilution (Red). Scale bar = 10 µm

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody - C-terminal (AB228936)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody - C-terminal (AB228936)

Immunohistochemical analysis of mouse testis tissue stained for BRD3 protein at nucleus with ab228936 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Western blot - Anti-BRD3 antibody - C-terminal (AB228936)
  • WB

Supplier Data

Western blot - Anti-BRD3 antibody - C-terminal (AB228936)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-BRD3 antibody - C-terminal (ab228936) at 1/20000 dilution

All lanes:

Mouse liver whole cell lysate at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 80 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human BRD3. The exact immunogen used to generate this antibody is proprietary information.

Q15059

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chromatin reader that recognizes and binds acetylated histones, thereby controlling gene expression and remodeling chromatin structures (PubMed : 18406326, PubMed : 22464331, PubMed : 27105114, PubMed : 32895492). Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation (PubMed : 29567837, PubMed : 32895492). In vitro, binds acetylated lysine residues on the N-terminus of histone H2A, H2B, H3 and H4 (PubMed : 18406326). Involved in endoderm differentiation via its association with long non-coding RNA (lncRNA) DIGIT : BRD3 undergoes liquid-liquid phase separation upon binding to lncRNA DIGIT, promoting binding to histone H3 acetylated at 'Lys-18' (H3K18ac) to induce endoderm gene expression (PubMed : 32895492). Also binds non-histones acetylated proteins, such as GATA1 and GATA2 : regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity).
See full target information BRD3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:6707 PubMed36344525

2022

SAMHD1 deacetylation by SIRT1 promotes DNA end resection by facilitating DNA binding at double-strand breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Priya Kapoor-Vazirani,Sandip K Rath,Xu Liu,Zhen Shu,Nicole E Bowen,Yitong Chen,Ramona Haji-Seyed-Javadi,Waaqo Daddacha,Elizabeth V Minten,Diana Danelia,Daniela Farchi,Duc M Duong,Nicholas T Seyfried,Xingming Deng,Eric A Ortlund,Baek Kim,David S Yu

Cell chemical biology 29:1470-1481.e31 PubMed36070758

2022

Exploring the target scope of KEAP1 E3 ligase-based PROTACs.

Applications

Unspecified application

Species

Unspecified reactive species

Guangyan Du,Jie Jiang,Nathaniel J Henning,Nozhat Safaee,Eriko Koide,Radosław P Nowak,Katherine A Donovan,Hojong Yoon,Inchul You,Hong Yue,Nicholas A Eleuteri,Zhixiang He,Zhengnian Li,Hubert T Huang,Jianwei Che,Behnam Nabet,Tinghu Zhang,Eric S Fischer,Nathanael S Gray
View all publications
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