Rabbit Polyclonal BRD4 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 32 publications.
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- Journal of translational medicine 21:7522023JMJD6-BRD4 complex stimulates lncRNA HOTAIR transcription by binding to the promoter region of HOTAIR and induces radioresistance in liver cancer stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuifeng Pei,Le Zhao,Yiren Ding,Zhan Su,Deqiang Li,Shuo Zhu,Lu Xu,Wei Zhao,Wuyuan ZhouPubMed 37880710
- Cell death discovery 9:2792023Bellidifolin ameliorates isoprenaline-induced cardiac hypertrophy by the Nox4/ROS signalling pathway through inhibiting BRD4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDingyan Zhou,Weizhe Liu,Juanjuan Zhang,Yucui Dong,Jiangli Wu,Yu Zhang,Cheng Dai,Tingting Zhang,Gaoshan Yang,Yue Zhang,Aiying LiPubMed 37528096
- NPJ precision oncology 7:112023Towards precision radiation oncology: endocrine therapy response as a biomarker for personalization of breast radiotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS M Nashir Udden,GuemHee Baek,Kamal Pandey,Chantal Vidal,Yulun Liu,Asal S Rahimi,D Nathan Kim,Chika R Nwachukwu,Ram S Mani,Prasanna G AlluriPubMed 36693944
- Stem cell research & therapy 13:3402022Pretreatment of nucleus pulposus mesenchymal stem cells with appropriate concentration of HO enhances their ability to treat intervertebral disc degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Yao Zhang,Zhi-Lei Hu,Yu-Han Qi,Hai-Yin Li,Xian Chang,Xiao-Xin Gao,Chen-Hao Liu,Yue-Yang Li,Jin-Hui Lou,Yu Zhai,Chang-Qing LiPubMed 35883157
- Journal of cardiovascular pharmacology 78:839-8462021BRD4 Inhibition Protects Against Myocardial Ischemia/Reperfusion Injury by Suppressing Inflammation and Oxidative Stress Through the PI3K/AKT Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongwei Wei,Qingjian Xue,Lei Sun,Jie LvPubMed 34524258
- Nucleic acids research 49:6281-62952021The H3K36me2 methyltransferase NSD1 modulates H3K27ac at active enhancers to safeguard gene expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Fang,Yin Tang,Yanjun Zhang,Yixin Pan,Junqi Jia,Zhongxing Sun,Weiwu Zeng,Jiaqi Chen,Ying Yuan,Dong FangPubMed 34107030
- Autophagy 18:391-4082021Epigenetic reader BRD4 supports mycobacterial pathogenesis by co-modulating host lipophagy and angiogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTanushree Mukherjee,Bharat Bhatt,Praveen Prakhar,Gaurav Kumar Lohia,R S Rajmani,Kithiganahalli Narayanaswamy BalajiPubMed 34074211
- Cell death & disease 12:5302021Pericentromeric Satellite III transcripts induce etoposide resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulian Kanne,Michelle Hussong,Jörg Isensee,Álvaro Muñoz-López,Jan Wolffgramm,Felix Heß,Christina Grimm,Sergey Bessonov,Lydia Meder,Jie Wang,H Christian Reinhardt,Margarete Odenthal,Tim Hucho,Reinhard Büttner,Daniel Summerer,Michal R SchweigerPubMed 34031359
- International journal of chronic obstructive pulmonary disease 16:919-9312021Circ-OSBPL2 Contributes to Smoke-Related Chronic Obstructive Pulmonary Disease by Targeting miR-193a-5p/BRD4 Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCaifen Zheng,Yongping Zhang,Yingchun Zhao,Yuanfang Duan,Qianghua Mu,Xinying WangPubMed 33854310
- Molecular medicine reports 19:5227-52362019miR‑125b‑mediated regulation of cell proliferation through the Jagged‑1/Notch signaling pathway by inhibiting BRD4 expression in psoriasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Pan et. al.PubMed 31059052
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
| Dog | Predicted | Predicted |
| Horse | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Dog | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Dog | Dilution info - | Notes - |
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Target data
Function
Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation (PubMed:20871596, PubMed:23086925, PubMed:23317504, PubMed:29176719, PubMed:29379197). Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure (PubMed:22334664, PubMed:23317504, PubMed:23589332). During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters (PubMed:16109376, PubMed:16109377, PubMed:19596240, PubMed:23589332, PubMed:24360279). Also recruits P-TEFb complex to distal enhancers, so called anti-pause enhancers in collaboration with JMJD6 (PubMed:16109376, PubMed:16109377, PubMed:19596240, PubMed:23589332, PubMed:24360279). BRD4 and JMJD6 are required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II (PubMed:16109376, PubMed:16109377, PubMed:19596240, PubMed:23589332, PubMed:24360279). Regulates differentiation of naive CD4(+) T-cells into T-helper Th17 by promoting recruitment of P-TEFb to promoters (By similarity). Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II (PubMed:23086925). According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II; these data however need additional evidences in vivo (PubMed:22509028). In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B (PubMed:19103749). Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters (PubMed:23317504). Isoform B. Acts as a chromatin insulator in the DNA damage response pathway. Inhibits DNA damage response signaling by recruiting the condensin-2 complex to acetylated histones, leading to chromatin structure remodeling, insulating the region from DNA damage response by limiting spreading of histone H2AX/H2A.x phosphorylation.
Alternative names
HUNK1, BRD4, Bromodomain-containing protein 4, Protein HUNK1
Recommended products
Rabbit Polyclonal BRD4 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 32 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Brd4 protein also known as Bromodomain containing protein 4 plays an important role as a transcriptional regulator. It recognizes acetylated lysine residues on histone tails through its bromodomains. This binding impacts chromatin structure and transcriptional activation. The Brd4 protein has a molecular weight of around 150 kDa and is often used as a marker in studies using Brd4 western blot techniques. It mainly expresses in the nucleus of most cell types and is an important focus for developing Brd4 anticuerpos useful for detecting protein size discrepancies in research.
- Biological function summary
Brd4 plays a significant role in regulating gene expression during cell cycle progression. It interacts with the Mediator complex enabling communication between transcriptional activators and RNA polymerase II. This protein is integral for maintaining chromatin in an active state ensuring accessibility for transcription machinery. Moreover Brd4 size and functionality relate to the elongation of transcription by RNA polymerase II ensuring the seamless expression of genes essential for cellular functions.
- Pathways
Brd4 integrates into transcriptional and epigenetic regulation pathways. It acts in the positive regulation of the MYC pathway directly linking Brd4 with proto-oncogene c-Myc. Also Brd4 engages in the JAK/STAT signaling pathway where its activity affects cell growth and immune response. These interactions establish Brd4 as a central regulator within these pathways facilitating communication and coordination among molecular players.
- Associated diseases and disorders
Brd4 link heavily to cancer and inflammatory diseases. Aberrant Brd4 activity often associates with various cancers including leukemia where its regulation impacts oncogenic drivers. Clinically targeting Brd4 presents a therapeutic avenue as its inhibition affects cyclin-dependent kinase 9 (CDK9) an important regulator of the cell cycle. Similarly Brd4 alterations implicate in inflammatory disorders impacting nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) signaling revealing pathways for intervening in pathogenic processes.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-Brd4 antibody (ab75898)
Lanes 1 - 2: Merged signal (red and green). Green - ab75898 observed at 220 kDa. Red - loading control Mouse anti Vinculin observed at 125 kDa.ab75898 was shown to react with Brd4 in HAP1 wild-type cells in Western blot with loss of signal observed in BRD4 knockout sample. Wild-type HAP1 and BRD4 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab75898 and Mouse anti Vinculin overnight at 4 °C at 1 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-Brd4 antibody (ab75898) at 1 µg/mL
Lane 1: Wild-type HAP1 cell lysate at 20 µg
Lane 2: BRD4 knockout HAP1 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 152 kDa
Observed band size: 220 kDa
Image courtesy of a customer review submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, CanadaImmunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody (ab75898)
ab75898 (1/500) staining Brd4 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
Western blot - Anti-Brd4 antibody (ab75898)
All lanes: Western blot - Anti-Brd4 antibody (ab75898) at 1 µg/mL
Lane 1: Liver (Mouse) Tissue Lysate at 10 µg/mL
Lane 2: Liver (Rat) Tissue Lysate at 10 µg/mL
Lane 3: Human liver tissue lysate - total protein (ab29889) at 10 µg/mL
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 152 kDa
Observed band size: 171 kDa, 70 kDa
Exposure time: 150s
Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody (ab75898)
ICC/IF image of ab75898 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75898, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293 and HepG2 cells at 5µg/ml.
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