AB243862

Anti-Brd4 antibody [BL-149-2H5]

Name: Anti-Brd4 antibody [BL-149-2H5] (ab243862) | Abcam
Brand: Abcam Limited
SKU: AB243862
Price: 400 USD
Availability: InStock
Rating: 5 (1 reviews)

Recombinant
Lab Essentials
Advanced Validation
What is this?

(1 Review)

(17 Publications)

Anti-Brd4 antibody [BL-149-2H5] (ab243862) is a rabbit monoclonal antibody detecting Brd4 in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF, ChIP. Suitable for Human, Mouse.

- Recombinant format for unrivalled batch-batch consistency

View Alternative Names

HUNK1, BRD4, Bromodomain-containing protein 4, Protein HUNK1

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [BL-149-2H5] (AB243862)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [BL-149-2H5] (AB243862)

Immunohistochemical analysis of Formalin-Fixed Paraffin-Embedded human breast carcinoma tissue labeling BRD4 with ab243862, followed by a HRP-conjugated goat anti-rabbit IgG as secondary. Substrate : DAB. Nuclear staining on human breast carcinoma is observed. Counter stained with hematoxylin. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes is recommended.

Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [BL-149-2H5] (AB243862)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [BL-149-2H5] (AB243862)

Formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling brd4 using ab243862 at 1/250 dilution in ICC/IF analysis. DyLight® 594-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Flow Cytometry - Anti-Brd4 antibody [BL-149-2H5] (AB243862)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Brd4 antibody [BL-149-2H5] (AB243862)

Flow cytometric analysis of 4% formaldehyde and permeabilized with 90% methanol KG-1 (Human acute myeloid leukemia cell line from bone marrow) cells labelling BRD4 with ab243862 at 1μl/10⁶ cells (Red shaded) compared with a Isotype control (unshaded). DyLight^® 650-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Supplier Data

Immunocytochemistry - Anti-Brd4 antibody [BL-149-2H5] (AB243862)

Immunocytochemical analysis of Formalin-Fixed Paraffin-Embedded HeLa cells labeling human BRD4 with ab243862, followed by a HRP-conjugated goat anti-rabbit IgG as secondary. Substrate : DAB. Nuclear staining on HeLa cells is observed. Counter stained with hematoxylin. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes is recommended.

ChIP-sequencing - Anti-Brd4 antibody [BL-149-2H5] (AB243862)

ChIP-seq

Unknown

ChIP-sequencing - Anti-Brd4 antibody [BL-149-2H5] (AB243862)

Localization of human BRD4 binding sites in immunoprecipitates from Mia PaCa-2 cells by ChIP-Seq using ab243862.

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Immunoprecipitation - Anti-Brd4 antibody [BL-149-2H5] (AB243862)

brd4 was immunoprecipitated from 1 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with ab243862 at 20 μL/mg lysate. Western blot was performed on the immunoprecipitate using ab243862 at 1/1000 dilution.

Lane 1 : ab243862 IP in HeLa cell lysate.

Lane 2 : Contol IgG in HeLa cell lysate.

All lanes:

Immunoprecipitation - Anti-Brd4 antibody [BL-149-2H5] (ab243862)

Predicted band size: 152 kDa

false

Supplier Data

Western blot - Anti-Brd4 antibody [BL-149-2H5] (AB243862)

Detection : Chemiluminescence.

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-Brd4 antibody [BL-149-2H5] (ab243862) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 15 µg

Lane 2:

HEK293T whole cell lysate at 15 µg

Lane 3:

Jurkat whole cell lysate at 15 µg

Lane 4:

MDA-MB-231 whole cell lysate at 15 µg

Lane 5:

A-549 whole cell lysate at 15 µg

Lane 6:

SW620 whole cell lysate at 15 µg

Lane 7:

SK-MEL-28 whole cell lysate at 15 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 152 kDa

false

Exposure time: 3s

Supplier Data

Western blot - Anti-Brd4 antibody [BL-149-2H5] (AB243862)

All lanes:

Western blot - Anti-Brd4 antibody [BL-149-2H5] (ab243862) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 25 µg

Lane 2:

HEK293T whole cell lysate at 25 µg

Lane 3:

Jurkat whole cell lysate at 25 µg

Lane 4:

A-549 whole cell lysate at 25 µg

Lane 5:

SW620 whole cell lysate at 25 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 152 kDa

Observed band size: 210 kDa

true

Exposure time: 10s

Supplier Data

Western blot - Anti-Brd4 antibody [BL-149-2H5] (AB243862)

All lanes:

Western blot - Anti-Brd4 antibody [BL-149-2H5] (ab243862) at 1/1000 dilution

Lane 1:

NIH 3T3 whole cell lysate at 10 µg

Lane 2:

CT26 whole cell lysate at 10 µg

Lane 3:

CH27 whole cell lysate at 10 µg

Lane 4:

TCMK-1 whole cell lysate at 10 µg

Lane 5:

BW5147.3 whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 155 kDa

Observed band size: 210 kDa

true

Exposure time: 10s

Supplier Data

Western blot - Anti-Brd4 antibody [BL-149-2H5] (AB243862)

Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Western blot - Anti-Brd4 antibody [BL-149-2H5] (ab243862) at 1/1000 dilution

All lanes:

BW5147.3 (Mouse T cell lymphoma cell line from thymus) whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG antibody

Predicted band size: 152 kDa

false

Unconjugated

Anti-Brd4 antibody [BL-149-2H5] - BSA free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BL-149-2H5

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB, IP, Flow Cyt, ICC/IF, ChIP-seq

applications

Immunogen

Synthetic Peptide within Human BRD4 aa 1300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O60885

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use 5-10μl/0.5mg lysate.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µL for 10^6 Cells", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "", "ChIPseq-species-checked": "testedAndGuaranteed", "ChIPseq-species-dilution-info": "10-40 µL for 30 µL chromatin", "ChIPseq-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Brd4 antibody [BL-149-2H5] (ab243862) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP in Human, Mouse samples.

Other related products
We have a range of other formats of antibody clone [BL-149-2H5] also available for your convenience: ab243862, ab272042

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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Properties and storage information

Form

Liquid

Purification notes

Recombinant antibody was purified from cell culture supernatant.

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Brd4 protein also known as Bromodomain containing protein 4 plays an important role as a transcriptional regulator. It recognizes acetylated lysine residues on histone tails through its bromodomains. This binding impacts chromatin structure and transcriptional activation. The Brd4 protein has a molecular weight of around 150 kDa and is often used as a marker in studies using Brd4 western blot techniques. It mainly expresses in the nucleus of most cell types and is an important focus for developing Brd4 anticuerpos useful for detecting protein size discrepancies in research.

Biological function summary

Brd4 plays a significant role in regulating gene expression during cell cycle progression. It interacts with the Mediator complex enabling communication between transcriptional activators and RNA polymerase II. This protein is integral for maintaining chromatin in an active state ensuring accessibility for transcription machinery. Moreover Brd4 size and functionality relate to the elongation of transcription by RNA polymerase II ensuring the seamless expression of genes essential for cellular functions.

Pathways

Brd4 integrates into transcriptional and epigenetic regulation pathways. It acts in the positive regulation of the MYC pathway directly linking Brd4 with proto-oncogene c-Myc. Also Brd4 engages in the JAK/STAT signaling pathway where its activity affects cell growth and immune response. These interactions establish Brd4 as a central regulator within these pathways facilitating communication and coordination among molecular players.

Brd4 link heavily to cancer and inflammatory diseases. Aberrant Brd4 activity often associates with various cancers including leukemia where its regulation impacts oncogenic drivers. Clinically targeting Brd4 presents a therapeutic avenue as its inhibition affects cyclin-dependent kinase 9 (CDK9) an important regulator of the cell cycle. Similarly Brd4 alterations implicate in inflammatory disorders impacting nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) signaling revealing pathways for intervening in pathogenic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation (PubMed : 20871596, PubMed : 23086925, PubMed : 23317504, PubMed : 29176719, PubMed : 29379197). Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure (PubMed : 22334664, PubMed : 23317504, PubMed : 23589332). During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters (PubMed : 16109376, PubMed : 16109377, PubMed : 19596240, PubMed : 23589332, PubMed : 24360279). Also recruits P-TEFb complex to distal enhancers, so called anti-pause enhancers in collaboration with JMJD6 (PubMed : 16109376, PubMed : 16109377, PubMed : 19596240, PubMed : 23589332, PubMed : 24360279). BRD4 and JMJD6 are required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II (PubMed : 16109376, PubMed : 16109377, PubMed : 19596240, PubMed : 23589332, PubMed : 24360279). Regulates differentiation of naive CD4(+) T-cells into T-helper Th17 by promoting recruitment of P-TEFb to promoters (By similarity). Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II (PubMed : 23086925). According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II; these data however need additional evidences in vivo (PubMed : 22509028). In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B (PubMed : 19103749). Also acts as a regulator of p53/TP53-mediated transcription : following phosphorylation by CK2, recruited to p53/TP53 specific target promoters (PubMed : 23317504).. Isoform B. Acts as a chromatin insulator in the DNA damage response pathway. Inhibits DNA damage response signaling by recruiting the condensin-2 complex to acetylated histones, leading to chromatin structure remodeling, insulating the region from DNA damage response by limiting spreading of histone H2AX/H2A.x phosphorylation.

See full target information BRD4

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Journal of hematology & oncology 18:67 PubMed40598222

2025

BRD4 acts as a transcriptional repressor of RhoB to inhibit terminal erythropoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yijin Chen,Dawei Huo,Ye Meng,Jie Zhang,Mengmeng Huang,Qian Luo,Yulin Xu,Haiqiong Zheng,Yingli Han,Xiangjun Zeng,Yanjuan Liu,Yunfei Liu,Rui Wen,Delin Kong,Ruxiu Tie,Shanshan Pei,Nan Liu,Pengxu Qian,He Huang,Meng Zhang

Cell death & disease 16:312 PubMed40246814

2025

USP9X integrates TGF-β and hypoxia signalings to promote ovarian cancer chemoresistance via HIF-2α-maintained stemness.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenlei Zhang,Xiujie Yu,Liqi Wen,Jia'nan Wang,Zhufeng Li,Yu Zhang,Jiayu Cheng,Ronglin Kan,Wanting Zhang,Yan Shen,Shukai Yuan,Li Zhao

BMC biology 22:195 PubMed39256730

2024

Early inhibition of BRD4 facilitates iPSC reprogramming via accelerating rDNA dynamic expression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhijing Zhang,Xinglin Hu,Yuchen Sun,Lei Lei,Zhonghua Liu

Journal of neuroinflammation 21:186 PubMed39080649

2024

Lactylation of histone by BRD4 regulates astrocyte polarization after experimental subarachnoid hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Zhang,Jian Zhou,Peng Lu,Xianhui Zhang,Lei Yang,Jinpeng Wu,Lihan Zhang,Lifang Zhang,Jinwei Pang,Huangfan Xie,Bingqing Xie,Yong Jiang,Jianhua Peng

Journal of experimental & clinical cancer research : CR 43:69 PubMed38443991

2024

KLF7 regulates super-enhancer-driven IGF2BP2 overexpression to promote the progression of head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hongshi Cai,Jianfeng Liang,Yaoqi Jiang,Ziyi Wang,Hongyu Li,Wenjin Wang,Cheng Wang,Jinsong Hou

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2308637 PubMed38417121

2024

Disruption of Super-Enhancers in Activated Pancreatic Stellate Cells Facilitates Chemotherapy and Immunotherapy in Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yazhou Wang,Kai Chen,Gang Liu,Chong Du,Zhaoxia Cheng,Dan Wei,Fenfen Li,Chen Li,Yinmo Yang,Ying Zhao,Guangjun Nie

Journal of chemotherapy (Florence, Italy) 37:45-59 PubMed38291982

2024

Self-assembled redox-responsive BRD4 siRNA nanoparticles: fomulation and its in vitro delivery in gastric cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mengying Zhang,Zhonghua An,Yiming Jiang,Meijiao Wei,Xiangbo Li,Yifan Wang,Hongbo Wang,Yanling Gong

Journal of orthopaedic surgery and research 18:876 PubMed37980502

2023

BRD4 facilitates osteogenic differentiation of human bone marrow mesenchymal stem cells through WNT4/NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Ning,Huihui Guo,Mingming Ma,Zhengang Zha

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2305279 PubMed37968249

2023

CAFs Homologous Biomimetic Liposome Bearing BET Inhibitor and Pirfenidone Synergistically Promoting Antitumor Efficacy in Pancreatic Ductal Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yin Zhang,Ranran Yu,Cheng Zhao,Jiawei Liang,Yixuan Zhang,Haochen Su,Jing Zhao,Hao Wu,Shijin Xu,Ziying Zhang,Lei Wang,Xiaoping Zou,Yun Zhu,Shu Zhang,Ying Lv

Cell & bioscience 13:166 PubMed37691108

2023

The SIRT7-mediated deacetylation of CHD1L amplifies HIF-2α-dependent signal that drives renal cell carcinoma progression and sunitinib resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Hongchao He,Jie Li,Wei Wang,Jie Cheng,Jian Zhou,Qunyi Li,Juan Jin,Li Chen

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Brd4 antibody [BL-149-2H5]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [BL-149-2H5] (AB243862)

Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [BL-149-2H5] (AB243862)

Flow Cytometry - Anti-Brd4 antibody [BL-149-2H5] (AB243862)

Immunocytochemistry - Anti-Brd4 antibody [BL-149-2H5] (AB243862)

ChIP-sequencing - Anti-Brd4 antibody [BL-149-2H5] (AB243862)

Immunoprecipitation - Anti-Brd4 antibody [BL-149-2H5] (AB243862)

Western blot - Anti-Brd4 antibody [BL-149-2H5] (AB243862)

Western blot - Anti-Brd4 antibody [BL-149-2H5] (AB243862)

Western blot - Anti-Brd4 antibody [BL-149-2H5] (AB243862)

Western blot - Anti-Brd4 antibody [BL-149-2H5] (AB243862)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

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