AB272042

Anti-Brd4 antibody [BL-149-2H5] - BSA free

Advanced Validation
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal BRD4 antibody. Suitable for IP, WB, ICC/IF, ChIP-seq and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human BRD4 aa 1300 to C-terminus.

View Alternative Names

HUNK1, BRD4, Bromodomain-containing protein 4, Protein HUNK1

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [BL-149-2H5] - BSA free (AB272042)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [BL-149-2H5] - BSA free (AB272042)

Formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling brd4 using ab243862 at 1/250 dilution in ICC/IF analysis. DyLight® 594-conjugated goat anti-rabbit IgG was used as the secondary antibody.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243862).

Unknown

Immunoprecipitation - Anti-Brd4 antibody [BL-149-2H5] - BSA free (AB272042)

brd4 was immunoprecipitated from 1 mg HeLa whole cell lysate with ab243862 at 20 μL/mg lysate. Western blot was performed on the immunoprecipitate using ab243862 at 1/1000 dilution.

Lane 1 : ab243862 IP in HeLa cell lysate.

Lane 2 : Contol IgG in HeLa cell lysate.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243862).

All lanes:

Immunoprecipitation - Anti-Brd4 antibody [BL-149-2H5] (<a href='/en-us/products/primary-antibodies/brd4-antibody-bl-149-2h5-ab243862'>ab243862</a>)

Predicted band size: 152 kDa

false

ChIP-sequencing - Anti-Brd4 antibody [BL-149-2H5] - BSA free (AB272042)

ChIP-seq

Unknown

ChIP-sequencing - Anti-Brd4 antibody [BL-149-2H5] - BSA free (AB272042)

Localization of human BRD4 binding sites in immunoprecipitates from Mia PaCa-2 cells by ChIP-Seq using ab243862.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243862).

Supplier Data

Western blot - Anti-Brd4 antibody [BL-149-2H5] - BSA free (AB272042)

Detection : Chemiluminescence.

Lysates prepared using NETN lysis buffer.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243862).

All lanes:

Western blot - Anti-Brd4 antibody [BL-149-2H5] (<a href='/en-us/products/primary-antibodies/brd4-antibody-bl-149-2h5-ab243862'>ab243862</a>) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 15 µg

Lane 2:

HEK293T whole cell lysate at 15 µg

Lane 3:

Jurkat whole cell lysate at 15 µg

Lane 4:

MDA-MB-231 whole cell lysate at 15 µg

Lane 5:

A-549 whole cell lysate at 15 µg

Lane 6:

SW620 whole cell lysate at 15 µg

Lane 7:

SK-MEL-28 whole cell lysate at 15 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 152 kDa

false

Exposure time: 3s

Supplier Data

Western blot - Anti-Brd4 antibody [BL-149-2H5] - BSA free (AB272042)

Chemiluminescence with an exposure time of 3 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243862).

All lanes:

Western blot - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042) at 1/1000 dilution

All lanes:

BW5147.3 (Mouse T cell lymphoma cell line from thymus) whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 152 kDa

false

Unconjugated

Anti-Brd4 antibody [BL-149-2H5]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BL-149-2H5

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ChIP-seq, ICC/IF, IP, WB

applications

Immunogen

Synthetic Peptide within Human BRD4 aa 1300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O60885

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "testedAndGuaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" } } }

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Product details

ab272042 is the BSA-free version of ab243862.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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Properties and storage information

Form

Liquid

Purification notes

Recombinant antibody was purified from cell culture supernatant.

Storage buffer

pH: 8.2 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Brd4 protein also known as Bromodomain containing protein 4 plays an important role as a transcriptional regulator. It recognizes acetylated lysine residues on histone tails through its bromodomains. This binding impacts chromatin structure and transcriptional activation. The Brd4 protein has a molecular weight of around 150 kDa and is often used as a marker in studies using Brd4 western blot techniques. It mainly expresses in the nucleus of most cell types and is an important focus for developing Brd4 anticuerpos useful for detecting protein size discrepancies in research.

Biological function summary

Brd4 plays a significant role in regulating gene expression during cell cycle progression. It interacts with the Mediator complex enabling communication between transcriptional activators and RNA polymerase II. This protein is integral for maintaining chromatin in an active state ensuring accessibility for transcription machinery. Moreover Brd4 size and functionality relate to the elongation of transcription by RNA polymerase II ensuring the seamless expression of genes essential for cellular functions.

Pathways

Brd4 integrates into transcriptional and epigenetic regulation pathways. It acts in the positive regulation of the MYC pathway directly linking Brd4 with proto-oncogene c-Myc. Also Brd4 engages in the JAK/STAT signaling pathway where its activity affects cell growth and immune response. These interactions establish Brd4 as a central regulator within these pathways facilitating communication and coordination among molecular players.

Brd4 link heavily to cancer and inflammatory diseases. Aberrant Brd4 activity often associates with various cancers including leukemia where its regulation impacts oncogenic drivers. Clinically targeting Brd4 presents a therapeutic avenue as its inhibition affects cyclin-dependent kinase 9 (CDK9) an important regulator of the cell cycle. Similarly Brd4 alterations implicate in inflammatory disorders impacting nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) signaling revealing pathways for intervening in pathogenic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation (PubMed : 20871596, PubMed : 23086925, PubMed : 23317504, PubMed : 29176719, PubMed : 29379197). Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure (PubMed : 22334664, PubMed : 23317504, PubMed : 23589332). During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters (PubMed : 16109376, PubMed : 16109377, PubMed : 19596240, PubMed : 23589332, PubMed : 24360279). Also recruits P-TEFb complex to distal enhancers, so called anti-pause enhancers in collaboration with JMJD6 (PubMed : 16109376, PubMed : 16109377, PubMed : 19596240, PubMed : 23589332, PubMed : 24360279). BRD4 and JMJD6 are required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II (PubMed : 16109376, PubMed : 16109377, PubMed : 19596240, PubMed : 23589332, PubMed : 24360279). Regulates differentiation of naive CD4(+) T-cells into T-helper Th17 by promoting recruitment of P-TEFb to promoters (By similarity). Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II (PubMed : 23086925). According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II; these data however need additional evidences in vivo (PubMed : 22509028). In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B (PubMed : 19103749). Also acts as a regulator of p53/TP53-mediated transcription : following phosphorylation by CK2, recruited to p53/TP53 specific target promoters (PubMed : 23317504).. Isoform B. Acts as a chromatin insulator in the DNA damage response pathway. Inhibits DNA damage response signaling by recruiting the condensin-2 complex to acetylated histones, leading to chromatin structure remodeling, insulating the region from DNA damage response by limiting spreading of histone H2AX/H2A.x phosphorylation.

See full target information BRD4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmaceutica Sinica. B 15:1415-1429 PubMed40370549

2025

Effective therapeutic targeting of tumor lineage plasticity in neuroendocrine prostate cancer by BRD4 inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Xiong Zhang,Yatian Yang,Hongye Zou,Yang Yang,Xingling Zheng,Eva Corey,Amina Zoubeidi,Nicolas Mitsiades,Ai-Ming Yu,Yuanpei Li,Hong-Wu Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Brd4 antibody [BL-149-2H5] - BSA free

Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [BL-149-2H5] - BSA free (AB272042)

Immunoprecipitation - Anti-Brd4 antibody [BL-149-2H5] - BSA free (AB272042)

ChIP-sequencing - Anti-Brd4 antibody [BL-149-2H5] - BSA free (AB272042)

Western blot - Anti-Brd4 antibody [BL-149-2H5] - BSA free (AB272042)

Western blot - Anti-Brd4 antibody [BL-149-2H5] - BSA free (AB272042)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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