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Rabbit Recombinant Monoclonal BRD4 antibody. Suitable for IP, WB, ICC/IF, ChIP-seq and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human BRD4 aa 1300 to C-terminus.

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Images

Immunoprecipitation - Anti-Brd4 antibody [BL-149-2H5] - BSA free (AB272042), expandable thumbnail
  • Western blot - Anti-Brd4 antibody [BL-149-2H5] - BSA free (AB272042), expandable thumbnail
  • ChIP-sequencing - Anti-Brd4 antibody [BL-149-2H5] - BSA free (AB272042), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [BL-149-2H5] - BSA free (AB272042), expandable thumbnail
  • Western blot - Anti-Brd4 antibody [BL-149-2H5] - BSA free (AB272042), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 8.2
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human BRD4 aa 1300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O60885

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFChIP-seq
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation (PubMed:20871596, PubMed:23086925, PubMed:23317504, PubMed:29176719, PubMed:29379197). Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure (PubMed:22334664, PubMed:23317504, PubMed:23589332). During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters (PubMed:16109376, PubMed:16109377, PubMed:19596240, PubMed:23589332, PubMed:24360279). Also recruits P-TEFb complex to distal enhancers, so called anti-pause enhancers in collaboration with JMJD6 (PubMed:16109376, PubMed:16109377, PubMed:19596240, PubMed:23589332, PubMed:24360279). BRD4 and JMJD6 are required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II (PubMed:16109376, PubMed:16109377, PubMed:19596240, PubMed:23589332, PubMed:24360279). Regulates differentiation of naive CD4(+) T-cells into T-helper Th17 by promoting recruitment of P-TEFb to promoters (By similarity). Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II (PubMed:23086925). According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II; these data however need additional evidences in vivo (PubMed:22509028). In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B (PubMed:19103749). Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters (PubMed:23317504). Isoform B. Acts as a chromatin insulator in the DNA damage response pathway. Inhibits DNA damage response signaling by recruiting the condensin-2 complex to acetylated histones, leading to chromatin structure remodeling, insulating the region from DNA damage response by limiting spreading of histone H2AX/H2A.x phosphorylation.

Alternative names

HUNK1, BRD4, Bromodomain-containing protein 4, Protein HUNK1

Recommended products

Rabbit Recombinant Monoclonal BRD4 antibody. Suitable for IP, WB, ICC/IF, ChIP-seq and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human BRD4 aa 1300 to C-terminus.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human BRD4 aa 1300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O60885
Clone number
BL-149-2H5
Concentration
Loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

ab272042 is the BSA-free version of Anti-Brd4 antibody [BL-149-2H5] ab243862.

This product is sold under License from Bethyl Laboratories, Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Brd4 protein also known as Bromodomain containing protein 4 plays an important role as a transcriptional regulator. It recognizes acetylated lysine residues on histone tails through its bromodomains. This binding impacts chromatin structure and transcriptional activation. The Brd4 protein has a molecular weight of around 150 kDa and is often used as a marker in studies using Brd4 western blot techniques. It mainly expresses in the nucleus of most cell types and is an important focus for developing Brd4 anticuerpos useful for detecting protein size discrepancies in research.

Biological function summary

Brd4 plays a significant role in regulating gene expression during cell cycle progression. It interacts with the Mediator complex enabling communication between transcriptional activators and RNA polymerase II. This protein is integral for maintaining chromatin in an active state ensuring accessibility for transcription machinery. Moreover Brd4 size and functionality relate to the elongation of transcription by RNA polymerase II ensuring the seamless expression of genes essential for cellular functions.

Pathways

Brd4 integrates into transcriptional and epigenetic regulation pathways. It acts in the positive regulation of the MYC pathway directly linking Brd4 with proto-oncogene c-Myc. Also Brd4 engages in the JAK/STAT signaling pathway where its activity affects cell growth and immune response. These interactions establish Brd4 as a central regulator within these pathways facilitating communication and coordination among molecular players.

Associated diseases and disorders

Brd4 link heavily to cancer and inflammatory diseases. Aberrant Brd4 activity often associates with various cancers including leukemia where its regulation impacts oncogenic drivers. Clinically targeting Brd4 presents a therapeutic avenue as its inhibition affects cyclin-dependent kinase 9 (CDK9) an important regulator of the cell cycle. Similarly Brd4 alterations implicate in inflammatory disorders impacting nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) signaling revealing pathways for intervening in pathogenic processes.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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5 product images

  • Immunoprecipitation - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042), expandable thumbnail

    Immunoprecipitation - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)

    brd4 was immunoprecipitated from 1 mg HeLa whole cell lysate with Anti-Brd4 antibody [BL-149-2H5] ab243862 at 20 μL/mg lysate. Western blot was performed on the immunoprecipitate using Anti-Brd4 antibody [BL-149-2H5] ab243862 at 1/1000 dilution.

    Lane 1: Anti-Brd4 antibody [BL-149-2H5] ab243862 IP in HeLa cell lysate.

    Lane 2: Contol IgG in HeLa cell lysate.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-Brd4 antibody [BL-149-2H5] ab243862).

    All lanes: Immunoprecipitation - Anti-Brd4 antibody [BL-149-2H5] (Anti-Brd4 antibody [BL-149-2H5] ab243862)

    Predicted band size: 152 kDa

  • Western blot - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042), expandable thumbnail

    Western blot - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)

    Detection: Chemiluminescence.

    Lysates prepared using NETN lysis buffer.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-Brd4 antibody [BL-149-2H5] ab243862).

    All lanes: Western blot - Anti-Brd4 antibody [BL-149-2H5] (Anti-Brd4 antibody [BL-149-2H5] ab243862) at 1/1000 dilution

    Lane 1: HeLa whole cell lysate at 15 µg

    Lane 2: HEK293T whole cell lysate at 15 µg

    Lane 3: Jurkat whole cell lysate at 15 µg

    Lane 4: MDA-MB-231 whole cell lysate at 15 µg

    Lane 5: A-549 whole cell lysate at 15 µg

    Lane 6: SW620 whole cell lysate at 15 µg

    Lane 7: SK-MEL-28 whole cell lysate at 15 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG

    Predicted band size: 152 kDa

    Exposure time: 3s

  • ChIP-sequencing - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042), expandable thumbnail

    ChIP-sequencing - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)

    Localization of human BRD4 binding sites in immunoprecipitates from Mia PaCa-2 cells by ChIP-Seq using Anti-Brd4 antibody [BL-149-2H5] ab243862.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-Brd4 antibody [BL-149-2H5] ab243862).

  • Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)

    Formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling brd4 using Anti-Brd4 antibody [BL-149-2H5] ab243862 at 1/250 dilution in ICC/IF analysis. DyLight® 594-conjugated goat anti-rabbit IgG was used as the secondary antibody.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-Brd4 antibody [BL-149-2H5] ab243862).

  • Western blot - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042), expandable thumbnail

    Western blot - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)

    Chemiluminescence with an exposure time of 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-Brd4 antibody [BL-149-2H5] ab243862).

    All lanes: Western blot - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042) at 1/1000 dilution

    All lanes: BW5147.3 (Mouse T cell lymphoma cell line from thymus) whole cell lysate at 50 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG

    Predicted band size: 152 kDa

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