Anti-BRD7 antibody [2D3] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] - BSA and Azide free (AB255776)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling BRD7 with ab252820 at 5.67μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on human colon carcinoma tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using citrate buffer (pH 6.0, epitope retrieval solution 1).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252820).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] - BSA and Azide free (AB255776)

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling BRD7 with ab252820 at 5.67μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on rat testis tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using citrate buffer (pH 6.0, epitope retrieval solution 1).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252820).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] - BSA and Azide free (AB255776)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling BRD7 with ab252820 at 5.67μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on mouse testis tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using citrate buffer (pH 6.0, epitope retrieval solution 1).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252820).

• WB

Supplier Data

Western blot - Anti-BRD7 antibody [2D3] - BSA and Azide free (AB255776)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 15 secs.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252820).

All lanes:

Western blot - Anti-BRD7 antibody [2D3] (<a href='/en-us/products/primary-antibodies/brd7-antibody-2d3-ab252820'>ab252820</a>) at 0.567 µg/mL

Lane 1:

C6 (rat glial tumor glial cell), whole cell lysate at 10 µg

Lane 2:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg

Lane 4:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/5000 dilution

Predicted band size: 74 kDa

false

• WB

Supplier Data

Western blot - Anti-BRD7 antibody [2D3] - BSA and Azide free (AB255776)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1-2 : 15 secs; Lane 3 : 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252820).

All lanes:

Western blot - Anti-BRD7 antibody [2D3] (<a href='/en-us/products/primary-antibodies/brd7-antibody-2d3-ab252820'>ab252820</a>) at 0.567 µg/mL

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/5000 dilution

Predicted band size: 74 kDa

false