Rat Recombinant Monoclonal BRD7 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] - BSA and Azide free (AB255776), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-2d3-bsa-and-azide-free-ab255776--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img16257.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] - BSA and Azide free (AB255776)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] - BSA and Azide free (AB255776), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-2d3-bsa-and-azide-free-ab255776--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91300.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] - BSA and Azide free (AB255776)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] - BSA and Azide free (AB255776), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-2d3-bsa-and-azide-free-ab255776--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112498.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] - BSA and Azide free (AB255776)")
![Western blot - Anti-BRD7 antibody [2D3] - BSA and Azide free (AB255776), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-2d3-bsa-and-azide-free-ab255776--western-blot-img54052.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BRD7 antibody [2D3] - BSA and Azide free (AB255776)")
![Western blot - Anti-BRD7 antibody [2D3] - BSA and Azide free (AB255776), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-2d3-bsa-and-azide-free-ab255776--western-blot-img83399.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BRD7 antibody [2D3] - BSA and Azide free (AB255776)")
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Tested |
| Rat | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5.67 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 5.67 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 5.67 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.567 µg/mL | Notes - |
Species Rat | Dilution info 0.567 µg/mL | Notes - |
Species Human | Dilution info 0.567 µg/mL | Notes - |
Target data
Function
Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24-mediated activation of transcriptional activation by AR (By similarity). Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase.
Alternative names
BP75, CELTIX1, BRD7, Bromodomain-containing protein 7, 75 kDa bromodomain protein, Protein CELTIX-1
Recommended products
Rat Recombinant Monoclonal BRD7 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 2D3
- Purification technique
- Ion exchange chromatography
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab255776 is the carrier-free version of Anti-BRD7 antibody [2D3] ab252820.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-2d3-bsa-and-azide-free-ab255776--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img16257.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776)
Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling BRD7 with Anti-BRD7 antibody [2D3] ab252820 at 5.67μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Nuclear staining on rat testis tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Heat mediated antigen retrieval using citrate buffer (pH 6.0, epitope retrieval solution 1).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BRD7 antibody [2D3] ab252820).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-2d3-bsa-and-azide-free-ab255776--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91300.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling BRD7 with Anti-BRD7 antibody [2D3] ab252820 at 5.67μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Nuclear staining on mouse testis tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Heat mediated antigen retrieval using citrate buffer (pH 6.0, epitope retrieval solution 1).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BRD7 antibody [2D3] ab252820).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-2d3-bsa-and-azide-free-ab255776--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112498.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling BRD7 with Anti-BRD7 antibody [2D3] ab252820 at 5.67μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Nuclear staining on human colon carcinoma tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Heat mediated antigen retrieval using citrate buffer (pH 6.0, epitope retrieval solution 1).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BRD7 antibody [2D3] ab252820).
![Western blot - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-2d3-bsa-and-azide-free-ab255776--western-blot-img54052.jpg "Western blot - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776)")
Western blot - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 15 secs.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BRD7 antibody [2D3] ab252820).
All lanes: Western blot - Anti-BRD7 antibody [2D3] (Anti-BRD7 antibody [2D3] ab252820) at 0.567 µg/mL
Lane 1: C6 (rat glial tumor glial cell), whole cell lysate at 10 µg
Lane 2: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg
Lane 3: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg
Lane 4: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/5000 dilution
Predicted band size: 74 kDa
![Western blot - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-2d3-bsa-and-azide-free-ab255776--western-blot-img83399.jpg "Western blot - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776)")
Western blot - Anti-BRD7 antibody [2D3] - BSA and Azide free (ab255776)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-2: 15 secs; Lane 3: 3 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BRD7 antibody [2D3] ab252820).
All lanes: Western blot - Anti-BRD7 antibody [2D3] (Anti-BRD7 antibody [2D3] ab252820) at 0.567 µg/mL
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2: PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 3: Mouse brain tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/5000 dilution
Predicted band size: 74 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com