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AB314049

Anti-BRD7 antibody [BLR220K] - BSA free

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Rabbit Recombinant Monoclonal BRD7 antibody. Suitable for Flow Cyt, WB and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human BRD7 aa 150-200.

View Alternative Names

BP75, CELTIX1, BRD7, Bromodomain-containing protein 7, 75 kDa bromodomain protein, Protein CELTIX-1

4 Images
Flow Cytometry - Anti-BRD7 antibody [BLR220K] - BSA free (AB314049)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-BRD7 antibody [BLR220K] - BSA free (AB314049)

Detection of human BRD7 (shaded) in HeLa cells by flow cytometry. Antibody : Rabbit anti-BRD7 recombinant monoclonal antibody [BLR220K] (A700-220) or isotype control (unshaded). Secondary : DyLight® 650-conjugated goat anti-rabbit IgG

Flow Cytometry - Anti-BRD7 antibody [BLR220K] - BSA free (AB314049)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-BRD7 antibody [BLR220K] - BSA free (AB314049)

Detection of mouse BRD7 (shaded) in EL4 cells by flow cytometry. Antibody : Rabbit anti-BRD7 recombinant monoclonal antibody [BLR220K] (A700-220) or isotype control (unshaded). Secondary : DyLight® 650-conjugated goat anti-rabbit IgG (A120-101D5).

Western blot - Anti-BRD7 antibody [BLR220K] - BSA free (AB314049)
  • WB

Supplier Data

Western blot - Anti-BRD7 antibody [BLR220K] - BSA free (AB314049)

Samples : cells prepared using NETN lysis buffer. Antibody : Rabbit anti-BRD7 recombinant monoclonal antibody [BLR220K] used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 75 seconds. Lower Panel : Rabbit anti-COPB2 antibody

All lanes:

Western blot - Anti-BRD7 antibody [BLR220K] - BSA free (ab314049) at 1/1000 dilution

Lane 1:

HeLa Whole cell at 10 µg

Lane 2:

HEK293T Whole cell lysate at 10 µg

Lane 3:

Jurkat Whole cell lysate at 10 µg

Lane 4:

HepG2 Whole cell lysate at 10 µg

Lane 5:

LNCaP Whole cell lysate at 10 µg

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Western blot - Anti-BRD7 antibody [BLR220K] - BSA free (AB314049)
  • WB

Supplier Data

Western blot - Anti-BRD7 antibody [BLR220K] - BSA free (AB314049)

Sample cells prepared using NETN lysis buffer. Antibody : Rabbit anti-BRD7 recombinant monoclonal antibody [BLR220K] used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel : Rabbit anti-COPB2 antibody

All lanes:

Western blot - Anti-BRD7 antibody [BLR220K] - BSA free (ab314049) at 1/1000 dilution

Lane 1:

CT26 Whole cell lysate at 10 µg

Lane 2:

NIH 3T3 Whole cell lysate at 10 µg

Lane 3:

CH27 Whole cell lysate at 10 µg

Lane 4:

A20 Whole cell lysate at 10 µg

Lane 5:

TCMK-1 Whole cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR220K

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human BRD7 aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

Q9NPI1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24-mediated activation of transcriptional activation by AR (By similarity). Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase.
See full target information BRD7
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Product promise

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