Anti-BRD7 antibody [EPR24342-71] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR24342-71] - BSA and Azide free (AB282014)

This data was developed using ab270982, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling BRD7 with ab270982 at 1/100 dilution (5.45 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human testis. The section was incubated with ab270982 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR24342-71] - BSA and Azide free (AB282014)

This data was developed using ab270982, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human breast tumor tissue labelling BRD7 with ab270982 at 1/100 dilution (5.45 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human breast tumor. The section was incubated with ab270982 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Lab

Western blot - Anti-BRD7 antibody [EPR24342-71] - BSA and Azide free (AB282014)

This data was developed using ab270982, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lysates were made freshly and used in WB test immediately to minimize protein degradation.

Exposure time : 59 seconds.

All lanes:

Western blot - Anti-BRD7 antibody [EPR24342-71] (<a href='/en-us/products/primary-antibodies/brd7-antibody-epr24342-71-ab270982'>ab270982</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 74 kDa

Observed band size: 85 kDa

false

• WB

Lab

Western blot - Anti-BRD7 antibody [EPR24342-71] - BSA and Azide free (AB282014)

This data was developed using ab270982, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression pattern is consistent with what has been described in the literature (PMID : 32028981).

Exposure time : 81 seconds.

All lanes:

Western blot - Anti-BRD7 antibody [EPR24342-71] (<a href='/en-us/products/primary-antibodies/brd7-antibody-epr24342-71-ab270982'>ab270982</a>) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 74 kDa

Observed band size: 85 kDa

false