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AB307141

Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free

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Rabbit Recombinant Monoclonal BRD7 antibody. Carrier free. Suitable for IP, IHC-P, WB and reacts with Rat, Human, Mouse samples.

View Alternative Names

BP75, CELTIX1, BRD7, Bromodomain-containing protein 7, 75 kDa bromodomain protein, Protein CELTIX-1

8 Images
Immunoprecipitation - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141)
  • IP

Supplier Data

Immunoprecipitation - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141)

This data was developed using ab307140, the same antibody clone in a different buffer formulation.

BRD7 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug with ab307140 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307140 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug
Lane 2 : ab307140 IP in HeLa whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307140 in Hela whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds

All lanes:

Immunoprecipitation - Anti-BRD7 antibody [EPR26709-4] (<a href='/en-us/products/primary-antibodies/brd7-antibody-epr26709-4-ab307140'>ab307140</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg

Lane 2:

HeLa whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 85 kDa

false

Exposure time: 180s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141)

This data was developed using ab307140, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling BRD7 with ab307140 at 1/100 (4.94 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on rat cerebrum. The section was incubated with ab307140 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141)

This data was developed using ab307140, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling BRD7 with ab307140 at 1/100 (4.94 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on mouse testis. The section was incubated with ab307140 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141)

This data was developed using ab307140, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling BRD7 with ab307140 at 1/100 (4.94 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on mouse cerebrum. The section was incubated with ab307140 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunoprecipitation - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141)
  • IP

Supplier Data

Immunoprecipitation - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141)

This data was developed using ab307140, the same antibody clone in a different buffer formulation.

BRD7 was immunoprecipitated from 0.35 mg PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate 10 ug with ab307140 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307140 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate 10 ug
Lane 2 : ab307140 IP in PC-12 whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307140 in PC-12 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds

All lanes:

Immunoprecipitation - Anti-BRD7 antibody [EPR26709-4] (<a href='/en-us/products/primary-antibodies/brd7-antibody-epr26709-4-ab307140'>ab307140</a>) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate 10 μg

Lane 2:

PC-12 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 85 kDa

false

Exposure time: 180s

Western blot - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141)
  • WB

Supplier Data

Western blot - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141)

This data was developed using 307140, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. 180 seconds Exposure time :

All lanes:

Western blot - Anti-BRD7 antibody [EPR26709-4] (<a href='/en-us/products/primary-antibodies/brd7-antibody-epr26709-4-ab307140'>ab307140</a>) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate 20μg

Lane 2:

Raw264.7 (mouse elson murine leukemia virus-induced tumor macrophage) whole cell lysate 20μg

Lane 3:

2.4G2 (rat B cell lymphoma B lymphocyte) whole cell lysate 20μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 85 kDa

false

Exposure time: 180s

Western blot - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141)
  • WB

Supplier Data

Western blot - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141)

This data was developed using 307140, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. 180 seconds Exposure time :

All lanes:

Western blot - Anti-BRD7 antibody [EPR26709-4] (<a href='/en-us/products/primary-antibodies/brd7-antibody-epr26709-4-ab307140'>ab307140</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 μg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate 20 μg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 20 μg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 85 kDa

false

Exposure time: 180s

Western blot - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141)
  • WB

Supplier Data

Western blot - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141)

This data was developed using 307140, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : Low espression : HepG2 (PMID : 19909775). 81 seconds Exposure time :

All lanes:

Western blot - Anti-BRD7 antibody [EPR26709-4] (<a href='/en-us/products/primary-antibodies/brd7-antibody-epr26709-4-ab307140'>ab307140</a>) at 1/1000 dilution

Lane 1:

SW620 (human colorectal adenocarcinoma epithelial cell) whole cell lysate 50 μg

Lane 2:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate 50 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 85 kDa

false

Exposure time: 81s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26709-4

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24-mediated activation of transcriptional activation by AR (By similarity). Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase.
See full target information BRD7
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com