Rabbit Recombinant Monoclonal BRD7 antibody. Carrier free. Suitable for IP, IHC-P, WB and reacts with Rat, Human, Mouse samples.
Images
![Immunoprecipitation - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-epr26709-4-bsa-and-azide-free-ab307141--immunoprecipitation-img406393.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141)")
![Immunoprecipitation - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-epr26709-4-bsa-and-azide-free-ab307141--immunoprecipitation-img406392.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141)")
![Western blot - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-epr26709-4-bsa-and-azide-free-ab307141--western-blot-img405435.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141)")
![Western blot - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-epr26709-4-bsa-and-azide-free-ab307141--western-blot-img405434.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141)")
![Western blot - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-epr26709-4-bsa-and-azide-free-ab307141--western-blot-img405433.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (AB307141)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ChIP | IP | Flow Cyt (Intra) | ICC/IF | IHC-Fr | IHC-P | WB | |
|---|---|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Expected | Not recommended | Not recommended | Not recommended | Tested | Tested |
| Rat | Not recommended | Tested | Not recommended | Not recommended | Not recommended | Tested | Tested |
ChIP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Target data
Function
Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24-mediated activation of transcriptional activation by AR (By similarity). Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase.
Alternative names
BP75, CELTIX1, BRD7, Bromodomain-containing protein 7, 75 kDa bromodomain protein, Protein CELTIX-1
Recommended products
Rabbit Recombinant Monoclonal BRD7 antibody. Carrier free. Suitable for IP, IHC-P, WB and reacts with Rat, Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26709-4
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunoprecipitation - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-epr26709-4-bsa-and-azide-free-ab307141--immunoprecipitation-img406393.jpg "Immunoprecipitation - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141)")
Immunoprecipitation - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141)
This data was developed using Anti-BRD7 antibody [EPR26709-4] ab307140, the same antibody clone in a different buffer formulation.
BRD7 was immunoprecipitated from 0.35 mg PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate 10 ug with Anti-BRD7 antibody [EPR26709-4] ab307140 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-BRD7 antibody [EPR26709-4] ab307140 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate 10 ug
Lane 2: ABAB307140 IP in PC-12 whole cell lysate
Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-BRD7 antibody [EPR26709-4] ab307140 in PC-12 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 180 secondsAll lanes: Immunoprecipitation - Anti-BRD7 antibody [EPR26709-4] (Anti-BRD7 antibody [EPR26709-4] ab307140) at 1/1000 dilution
Lane 1: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate 10 μg
Lane 2: PC-12 whole cell lysate
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 85 kDa
Exposure time: 180s
![Immunoprecipitation - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-epr26709-4-bsa-and-azide-free-ab307141--immunoprecipitation-img406392.jpg "Immunoprecipitation - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141)")
Immunoprecipitation - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141)
This data was developed using Anti-BRD7 antibody [EPR26709-4] ab307140, the same antibody clone in a different buffer formulation.
BRD7 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug with Anti-BRD7 antibody [EPR26709-4] ab307140 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-BRD7 antibody [EPR26709-4] ab307140 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug
Lane 2: ABAB307140 IP in HeLa whole cell lysate
Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-BRD7 antibody [EPR26709-4] ab307140 in Hela whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 180 secondsAll lanes: Immunoprecipitation - Anti-BRD7 antibody [EPR26709-4] (Anti-BRD7 antibody [EPR26709-4] ab307140) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg
Lane 2: HeLa whole cell lysate
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 85 kDa
Exposure time: 180s
![Western blot - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-epr26709-4-bsa-and-azide-free-ab307141--western-blot-img405435.jpg "Western blot - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141)")
Western blot - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141)
This data was developed using 307140, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Low espression: HepG2 (PMID: 19909775).
81 seconds
Exposure time:All lanes: Western blot - Anti-BRD7 antibody [EPR26709-4] (Anti-BRD7 antibody [EPR26709-4] ab307140) at 1/1000 dilution
Lane 1: SW620 (human colorectal adenocarcinoma epithelial cell) whole cell lysate 50 μg
Lane 2: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate 50 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 85 kDa
Exposure time: 81s
![Western blot - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-epr26709-4-bsa-and-azide-free-ab307141--western-blot-img405434.jpg "Western blot - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141)")
Western blot - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141)
This data was developed using 307140, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
180 seconds
Exposure time:All lanes: Western blot - Anti-BRD7 antibody [EPR26709-4] (Anti-BRD7 antibody [EPR26709-4] ab307140) at 1/1000 dilution
Lane 1: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate 20μg
Lane 2: Raw264.7 (mouse elson murine leukemia virus-induced tumor macrophage) whole cell lysate 20μg
Lane 3: 2.4G2 (rat B cell lymphoma B lymphocyte) whole cell lysate 20μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 85 kDa
Exposure time: 180s
![Western blot - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-epr26709-4-bsa-and-azide-free-ab307141--western-blot-img405433.jpg "Western blot - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141)")
Western blot - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141)
This data was developed using 307140, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
180 seconds
Exposure time:All lanes: Western blot - Anti-BRD7 antibody [EPR26709-4] (Anti-BRD7 antibody [EPR26709-4] ab307140) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 μg
Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate 20 μg
Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 20 μg
Lane 4: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 85 kDa
Exposure time: 180s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-epr26709-4-bsa-and-azide-free-ab307141--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405430.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141)
This data was developed using Anti-BRD7 antibody [EPR26709-4] ab307140, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling BRD7 with Anti-BRD7 antibody [EPR26709-4] ab307140 at 1/100 (4.94 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on rat cerebrum. The section was incubated with Anti-BRD7 antibody [EPR26709-4] ab307140 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-epr26709-4-bsa-and-azide-free-ab307141--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405429.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141)
This data was developed using Anti-BRD7 antibody [EPR26709-4] ab307140, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling BRD7 with Anti-BRD7 antibody [EPR26709-4] ab307140 at 1/100 (4.94 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on mouse testis. The section was incubated with Anti-BRD7 antibody [EPR26709-4] ab307140 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-epr26709-4-bsa-and-azide-free-ab307141--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405428.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141)
This data was developed using Anti-BRD7 antibody [EPR26709-4] ab307140, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling BRD7 with Anti-BRD7 antibody [EPR26709-4] ab307140 at 1/100 (4.94 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on mouse cerebrum. The section was incubated with Anti-BRD7 antibody [EPR26709-4] ab307140 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-epr26709-4-bsa-and-azide-free-ab307141--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405430.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-epr26709-4-bsa-and-azide-free-ab307141--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405429.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141), expandable thumbnail](https://content.abcam.com/products/images/brd7-antibody-epr26709-4-bsa-and-azide-free-ab307141--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405428.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] - BSA and Azide free (ab307141)")
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