Rabbit Polyclonal BRD8/p120 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human BRD8 aa 200-300.
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Publications
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- Nature 613:195-2022022BRD8 maintains glioblastoma by epigenetic reprogramming of the p53 network.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXueqin Sun,Olaf Klingbeil,Bin Lu,Caizhi Wu,Carlos Ballon,Meng Ouyang,Xiaoli S Wu,Ying Jin,Yon Hwangbo,Yu-Han Huang,Tim D D Somerville,Kenneth Chang,Jung Park,Taemoon Chung,Scott K Lyons,Junwei Shi,Hannes Vogel,Michael Schulder,Christopher R Vakoc,Alea A MillsPubMed 36544023
- Endocrinology 153:5796-8082012The p400/Brd8 chromatin remodeling complex promotes adipogenesis by incorporating histone variant H2A.Z at PPARγ target genes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJean-Philippe Couture et. al.PubMed 23064015
- British journal of cancer 102:325-312010C20orf20 (MRG-binding protein) as a potential therapeutic target for colorectal cancer.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human BRD8 aa 200-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H0E9
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Rat | Predicted | Predicted | Predicted |
| Chinese hamster | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Elephant | Predicted | Predicted | Predicted |
| Gorilla | Predicted | Predicted | Predicted |
| Guinea pig | Predicted | Predicted | Predicted |
| Horse | Predicted | Predicted | Predicted |
| Opossum | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Rhesus monkey, Gorilla, Opossum, Chinese hamster, Elephant | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Rhesus monkey, Gorilla, Opossum, Chinese hamster, Elephant | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000.00000 - 1/25000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Rhesus monkey, Gorilla, Opossum, Chinese hamster, Elephant | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
May act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Isoform 2 stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. At least isoform 1 and isoform 2 are components of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome.
Alternative names
SMAP, SMAP2, BRD8, Bromodomain-containing protein 8, Skeletal muscle abundant protein, Skeletal muscle abundant protein 2, Thyroid hormone receptor coactivating protein of 120 kDa, p120, TrCP120
Recommended products
Rabbit Polyclonal BRD8/p120 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human BRD8 aa 200-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human BRD8 aa 200-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H0E9
- Purification technique
- Affinity purification Immunogen
- Specificity
Specific to p120
- Concentration
- Purification notes
Antibody was affinity purified using an epitope specific to BRD8/p120 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Immunoprecipitation - Anti-BRD8/p120 antibody (ab17969)
ab17969 immunoprecipitating BRD8 at 6 μg per reaction.
Lane 1: ab17969 in HeLa nuclear extract (0.5 or 1 mg per IP reaction; 20% of IP loaded).
Lane 2: IgG control
For blotting immunoprecipitated BRD8, ab17969 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
All lanes: Immunoprecipitation - Anti-BRD8/p120 antibody (ab17969)
Predicted band size: 135 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD8/p120 antibody (ab17969)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BRD8 / p120 with ab17969 at 1/1000 (1μg/ml). Detection: DAB.
Western blot - Anti-BRD8/p120 antibody (ab17969)
Detection of human BRD8 by Western Blot. Sample is nuclear
extract (NE; 5, 15, and 50mcg) from HeLa cells.All lanes: Western blot - Anti-BRD8/p120 antibody (ab17969)
Predicted band size: 135 kDa
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