JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB17969

Anti-BRD8/p120 antibody

Be the first to review this product! Submit a review

|

(7 Publications)

Rabbit Polyclonal BRD8/p120 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human BRD8 aa 200-300.

View Alternative Names

SMAP, SMAP2, BRD8, Bromodomain-containing protein 8, Skeletal muscle abundant protein, Skeletal muscle abundant protein 2, Thyroid hormone receptor coactivating protein of 120 kDa, p120, TrCP120

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD8/p120 antibody (AB17969)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD8/p120 antibody (AB17969)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BRD8 / p120 with ab17969 at 1/1000 (1μg/ml). Detection : DAB.

Immunoprecipitation - Anti-BRD8/p120 antibody (AB17969)
  • IP

Supplier Data

Immunoprecipitation - Anti-BRD8/p120 antibody (AB17969)

ab17969 immunoprecipitating BRD8 at 6 μg per reaction.

Lane 1 : ab17969 in HeLa nuclear extract (0.5 or 1 mg per IP reaction; 20% of IP loaded).

Lane 2 : IgG control

For blotting immunoprecipitated BRD8, ab17969 was used at 1 μg/mL. Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-BRD8/p120 antibody (ab17969)

Predicted band size: 135 kDa

false

Western blot - Anti-BRD8/p120 antibody (AB17969)
  • WB

Unknown

Western blot - Anti-BRD8/p120 antibody (AB17969)

Detection of human BRD8 by Western Blot. Sample is nuclear
extract (NE; 5, 15, and 50mcg) from HeLa cells.

All lanes:

Western blot - Anti-BRD8/p120 antibody (ab17969)

Predicted band size: 135 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, IP

applications

Immunogen

Synthetic Peptide within Human BRD8 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

Q9H0E9

Specificity

Specific to p120

style
left-column

Alternative Products

Primary Antibodies

AB17968

Anti-BRD8/p120 antibody

Western blot - Anti-BRD8/p120 antibody (AB17968)

  • 0
  • 0
Primary Antibodies

AB217850

Anti-BRD8/p120 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD8/p120 antibody (AB217850)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB4174

Anti-Histone H2A.Z antibody - ChIP Grade

Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (AB4174)

  • 4
  • 31
Primary Antibodies

AB20666

Anti-hnRNP U/p120 antibody

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP U/p120 antibody (AB20666)

  • 5
  • 3
Primary Antibodies

AB124762

Anti-p63 antibody [EPR5701]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody [EPR5701] (AB124762)

  • 4
  • 12
Primary Antibodies

AB191080

Anti-KMT6 / EZH2 antibody [EPR9307(2)] - N-terminal

RabMAb|Recombinant|KO Validated|Trial Size

Flow Cytometry (Intracellular) - Anti-KMT6 / EZH2 antibody [EPR9307(2)] - N-terminal (AB191080)

  • 2
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Opossum": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to BRD8/p120 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Do Not Freeze
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

May act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Isoform 2 stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. At least isoform 1 and isoform 2 are components of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome.
See full target information BRD8
style
left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 16:1419 PubMed40720004

2025

BRD8 inhibits colorectal cancer progression through TGF-β1-mediated tumor-associated macrophage recruitment.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Xu,Yejia Mo,Bingqi Yu,Sunfu Fan

Nutrients 17: PubMed40077701

2025

TW01 Mitigates PM-Induced Lung Injury and Improves Gut Health in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Siou-Min Luo,Ming-Ju Chen

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2409160 PubMed39656858

2024

BRD8 Guards the Pluripotent State by Sensing and Maintaining Histone Acetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Li Sun,Xiuling Fu,Zhen Xiao,Gang Ma,Yibin Zhou,Haoqing Hu,Liyang Shi,Dongwei Li,Ralf Jauch,Andrew Paul Hutchins

Cancers 16: PubMed38893094

2024

TWIST1 Drives Cytotoxic CD8+ T-Cell Exhaustion through Transcriptional Activation of (PD-L1) Expression in Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobin Yu,Jianming Xu

Nature 613:195-202 PubMed36544023

2022

BRD8 maintains glioblastoma by epigenetic reprogramming of the p53 network.

Applications

Unspecified application

Species

Unspecified reactive species

Xueqin Sun,Olaf Klingbeil,Bin Lu,Caizhi Wu,Carlos Ballon,Meng Ouyang,Xiaoli S Wu,Ying Jin,Yon Hwangbo,Yu-Han Huang,Tim D D Somerville,Kenneth Chang,Jung Park,Taemoon Chung,Scott K Lyons,Junwei Shi,Hannes Vogel,Michael Schulder,Christopher R Vakoc,Alea A Mills

Endocrinology 153:5796-808 PubMed23064015

2012

The p400/Brd8 chromatin remodeling complex promotes adipogenesis by incorporating histone variant H2A.Z at PPARγ target genes.

Applications

Unspecified application

Species

Unspecified reactive species

Jean-Philippe Couture,Guylaine Nolet,Elaine Beaulieu,Richard Blouin,Nicolas Gévry

British journal of cancer 102:325-31 PubMed20051959

2010

C20orf20 (MRG-binding protein) as a potential therapeutic target for colorectal cancer.

Applications

IP, WB

Species

Human, Human

K Yamaguchi,M Sakai,T Shimokawa,Y Yamada,Y Nakamura,Y Furukawa
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com