Anti-BRD9 antibody [BLR153J] - BSA free (ab314091)

Rabbit Recombinant Monoclonal BRD9 antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human BRD9 aa 500 to C-terminus.

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Images

Flow Cytometry - Anti-BRD9 antibody [BLR153J] - BSA free (AB314091), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Form: Liquid
Clonality: Monoclonal

Immunogen

Synthetic Peptide within Human BRD9 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H8M2

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Flow Cyt	ICC	IHC-P	IP	WB
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Tested	Expected	Tested	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Target data

See full target information BRD9

Function

Plays a role in chromatin remodeling and regulation of transcription (PubMed:22464331, PubMed:26365797). Acts as a chromatin reader that recognizes and binds acylated histones: binds histones that are acetylated and/or butyrylated (PubMed:26365797). Component of SWI/SNF chromatin remodeling subcomplex GBAF that carries out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner (PubMed:29374058). Orchestrates also the RAD51-RAD54 complex formation and thereby plays a role in homologous recombination (HR) (PubMed:32457312).

Alternative names

UNQ3040/PRO9856, BRD9, Bromodomain-containing protein 9, Rhabdomyosarcoma antigen MU-RMS-40.8

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Form: Liquid
Clonality: Monoclonal
Immunogen: Synthetic Peptide within Human BRD9 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H8M2
Clone number: BLR153J
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: Up to 12 months
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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9 product images

Flow Cytometry - Anti-BRD9 antibody [BLR153J] - BSA free (ab314091)
Detection of human BRD9 (shaded) in HDLM-2 cells by flow cytometry.Antibody:Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J] (A700-153) or isotype control (unshaded). Secondary:DyLight® 650-conjugated goat anti-rabbit IgG.
Flow Cytometry - Anti-BRD9 antibody [BLR153J] - BSA free (ab314091)
Detection of mouse BRD9 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J] (A700-153) or isotype control (unshaded). Secondary: 650-conjugated goat anti-rabbit IgG.
Western blot - Anti-BRD9 antibody [BLR153J] - BSA free (ab314091)
Samples: Whole cell lysate (50 µg) prepared using NETN buffer from Jurkat cells (WT and KO). Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J] (A700-153) used at 1:1000. Secondary: HRP-conjugated goat antirabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].
All lanes: Western blot - Anti-BRD9 antibody [BLR153J] - BSA free (ab314091) at 1/1000 dilution
Lane 1: Jurkat WT Whole cell lysate at 50 µg
Lane 2: Jurkat KO Whole cell lysate at 50 µg
Secondary
All lanes: HRP-conjugated goat antirabbit IgG
Western blot - Anti-BRD9 antibody [BLR153J] - BSA free (ab314091)
Samples: Whole cell lysate (30 µg) from Jurkat, HEK293T, K-562, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J] (A700-153) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody.
All lanes: Western blot - Anti-BRD9 antibody [BLR153J] - BSA free (ab314091) at 1/1000 dilution
Lane 1: Jurkat Whole cell lysate at 30 µg
Lane 2: HEK293T Whole cell lysate at 30 µg
Lane 3: K-562 Whole cell lysate at 30 µg
Lane 4: MCF-7 Whole cell lysate at 30 µg
Lane 5: HeLa Whole cell lysate at 30 µg
Secondary
All lanes: HRP-conjugated goat anti-rabbit IgG
Western blot - Anti-BRD9 antibody [BLR153J] - BSA free (ab314091)
Samples: Whole cell lysate (30 µg) from CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit antiBRD9 recombinant monoclonal antibody [BLR153J] (A700-153) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].
All lanes: Western blot - Anti-BRD9 antibody [BLR153J] - BSA free (ab314091) at 1/1000 dilution
Lane 1: CT26 Whole cell lysate at 30 µg
Lane 2: CH27 Whole cell lysate at 30 µg
Lane 3: TCMK-1 Whole cell lysate at 30 µg
Secondary
All lanes: HRP-conjugated goat anti-rabbit IgG
Immunoprecipitation - Anti-BRD9 antibody [BLR153J] - BSA free (ab314091)
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J] (A700-153-2) used for IP at 20 µl/mg lysate. BRD9 was also immunoprecipitated by a previous lot of this antibody (A700-153-1) and a second antibody against a different epitope of BRD9 (A303-781A). For blotting immunoprecipitated BRD9, A700-153 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [BLR153J] - BSA free (ab314091)
Sample: FFPE section of mouse plasmacytoma. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J] (A700-153). Secondary: HRP-conjugated goat anti-rabbit IgG.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [BLR153J] - BSA free (ab314091)
Detection of human BRD9 by immunohistochemistry. Sample: FFPE section of lung carcinoma. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J] (A700-153). Secondary: HRP-conjugated goat antirabbit IgG.
Immunocytochemistry - Anti-BRD9 antibody [BLR153J] - BSA free (ab314091)
Sample: FFPE section of HDLM-2 cells. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J] (A700-153). Secondary :HRP-conjugated goat antirabbit IgG.