AB314091

Anti-BRD9 antibody [BLR153J] - BSA free

Recombinant
KO Validated
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Rabbit Recombinant Monoclonal BRD9 antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human BRD9 aa 500 to C-terminus.

View Alternative Names

UNQ3040/PRO9856, BRD9, Bromodomain-containing protein 9, Rhabdomyosarcoma antigen MU-RMS-40.8

9 Images

Supplier Data

Immunocytochemistry - Anti-BRD9 antibody [BLR153J] - BSA free (AB314091)

Sample : FFPE section of HDLM-2 cells. Antibody : Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J] (A700-153). Secondary : HRP-conjugated goat antirabbit IgG.

Flow Cytometry - Anti-BRD9 antibody [BLR153J] - BSA free (AB314091)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-BRD9 antibody [BLR153J] - BSA free (AB314091)

Detection of human BRD9 (shaded) in HDLM-2 cells by flow cytometry.Antibody : Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J] (A700-153) or isotype control (unshaded). Secondary : DyLight® 650-conjugated goat anti-rabbit IgG.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [BLR153J] - BSA free (AB314091)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [BLR153J] - BSA free (AB314091)

Detection of human BRD9 by immunohistochemistry. Sample : FFPE section of lung carcinoma. Antibody : Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J] (A700-153). Secondary : HRP-conjugated goat antirabbit IgG.

Supplier Data

Immunoprecipitation - Anti-BRD9 antibody [BLR153J] - BSA free (AB314091)

Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies : Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J] (A700-153-2) used for IP at 20 µl/mg lysate. BRD9 was also immunoprecipitated by a previous lot of this antibody (A700-153-1) and a second antibody against a different epitope of BRD9 (A303-781A). For blotting immunoprecipitated BRD9, A700-153 was used at 1 : 1000. Detection : Chemiluminescence with an exposure time of 30 seconds.

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IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [BLR153J] - BSA free (AB314091)

Sample : FFPE section of mouse plasmacytoma. Antibody : Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J] (A700-153). Secondary : HRP-conjugated goat anti-rabbit IgG.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-BRD9 antibody [BLR153J] - BSA free (AB314091)

Detection of mouse BRD9 (shaded) in NIH3T3 cells by flow cytometry. Antibody : Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J] (A700-153) or isotype control (unshaded). Secondary : 650-conjugated goat anti-rabbit IgG.

Supplier Data

Western blot - Anti-BRD9 antibody [BLR153J] - BSA free (AB314091)

Samples : Whole cell lysate (30 µg) from Jurkat, HEK293T, K-562, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody : Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J] (A700-153) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Detection : Chemiluminescence with an exposure time of 3 minutes. Lower Panel : Rabbit anti-COPB2 antibody.

All lanes:

Western blot - Anti-BRD9 antibody [BLR153J] - BSA free (ab314091) at 1/1000 dilution

Lane 1:

Jurkat Whole cell lysate at 30 µg

Lane 2:

HEK293T Whole cell lysate at 30 µg

Lane 3:

K-562 Whole cell lysate at 30 µg

Lane 4:

MCF-7 Whole cell lysate at 30 µg

Lane 5:

HeLa Whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Supplier Data

Western blot - Anti-BRD9 antibody [BLR153J] - BSA free (AB314091)

Samples : Whole cell lysate (50 µg) prepared using NETN buffer from Jurkat cells (WT and KO). Antibody : Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J] (A700-153) used at 1 : 1000. Secondary : HRP-conjugated goat antirabbit IgG. Detection : Chemiluminescence with an exposure time of 3 minutes. Lower Panel : Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].

All lanes:

Western blot - Anti-BRD9 antibody [BLR153J] - BSA free (ab314091) at 1/1000 dilution

Lane 1:

Jurkat WT Whole cell lysate at 50 µg

Lane 2:

Jurkat KO Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat antirabbit IgG

false

Supplier Data

Western blot - Anti-BRD9 antibody [BLR153J] - BSA free (AB314091)

Samples : Whole cell lysate (30 µg) from CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody : Rabbit antiBRD9 recombinant monoclonal antibody [BLR153J] (A700-153) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Detection : Chemiluminescence with an exposure time of 3 minutes. Lower Panel : Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].

All lanes:

Western blot - Anti-BRD9 antibody [BLR153J] - BSA free (ab314091) at 1/1000 dilution

Lane 1:

CT26 Whole cell lysate at 30 µg

Lane 2:

CH27 Whole cell lysate at 30 µg

Lane 3:

TCMK-1 Whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR153J

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

IHC-P, WB, Flow Cyt, IP, ICC

applications

Immunogen

Synthetic Peptide within Human BRD9 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H8M2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in chromatin remodeling and regulation of transcription (PubMed : 22464331, PubMed : 26365797). Acts as a chromatin reader that recognizes and binds acylated histones : binds histones that are acetylated and/or butyrylated (PubMed : 26365797). Component of SWI/SNF chromatin remodeling subcomplex GBAF that carries out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner (PubMed : 29374058). Orchestrates also the RAD51-RAD54 complex formation and thereby plays a role in homologous recombination (HR) (PubMed : 32457312).

See full target information BRD9

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BRD9 antibody [BLR153J] - BSA free

Immunocytochemistry - Anti-BRD9 antibody [BLR153J] - BSA free (AB314091)

Flow Cytometry - Anti-BRD9 antibody [BLR153J] - BSA free (AB314091)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [BLR153J] - BSA free (AB314091)

Immunoprecipitation - Anti-BRD9 antibody [BLR153J] - BSA free (AB314091)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [BLR153J] - BSA free (AB314091)

Flow Cytometry - Anti-BRD9 antibody [BLR153J] - BSA free (AB314091)

Western blot - Anti-BRD9 antibody [BLR153J] - BSA free (AB314091)

Western blot - Anti-BRD9 antibody [BLR153J] - BSA free (AB314091)

Western blot - Anti-BRD9 antibody [BLR153J] - BSA free (AB314091)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise