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Rabbit Recombinant Monoclonal BRD9 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications.


Images

Western blot - Anti-BRD9 antibody [EPR23888-5] (AB259839), expandable thumbnail
  • Western blot - Anti-BRD9 antibody [EPR23888-5] (AB259839), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [EPR23888-5] (AB259839), expandable thumbnail
  • Western blot - Anti-BRD9 antibody [EPR23888-5] (AB259839), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [EPR23888-5] (AB259839), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPChIPWBIHC-PFlow Cyt (Intra)
Human
Not recommended
Not recommended
Not recommended
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Tested
Tested
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Tested
Tested
Not recommended

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Species
Rat
Dilution info
1/500
Notes

-

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

Plays a role in chromatin remodeling and regulation of transcription (PubMed:22464331, PubMed:26365797). Acts as a chromatin reader that recognizes and binds acylated histones: binds histones that are acetylated and/or butyrylated (PubMed:26365797). Component of SWI/SNF chromatin remodeling subcomplex GBAF that carries out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner (PubMed:29374058). Orchestrates also the RAD51-RAD54 complex formation and thereby plays a role in homologous recombination (HR) (PubMed:32457312).

Alternative names

UNQ3040/PRO9856, BRD9, Bromodomain-containing protein 9, Rhabdomyosarcoma antigen MU-RMS-40.8

Recommended products

Rabbit Recombinant Monoclonal BRD9 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23888-5
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-BRD9 antibody [EPR23888-5] (ab259839), expandable thumbnail

    Western blot - Anti-BRD9 antibody [EPR23888-5] (ab259839)

    False colour image of Western blot: Anti-BRD9 antibody [EPR23888-5] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab259839 was shown to bind specifically to BRD9. A band was observed at 75 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in BRD9 knockout cell line Human BRD9 knockout HEK-293T cell line ab266763 (knockout cell lysate Human BRD9 knockout HEK-293T cell lysate ab258336). To generate this image, wild-type and BRD9 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-BRD9 antibody [EPR23888-5] (ab259839) at 1/1000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: BRD9 knockout HEK-293T cell lysate at 20 µg

    Lane 2: Western blot - Human BRD9 knockout HEK-293T cell line (Human BRD9 knockout HEK-293T cell line ab266763)

    Performed under reducing conditions.

    Predicted band size: 67 kDa

    Observed band size: 75 kDa

  • Western blot - Anti-BRD9 antibody [EPR23888-5] (ab259839), expandable thumbnail

    Western blot - Anti-BRD9 antibody [EPR23888-5] (ab259839)

    Blocking and diluting buffer and concentration: 5% NFDM/TBSTLysates used in this blot have experienced freeze-thaw cycles.

    Exposure time: Lanes 1-2: 37 seconds;Lanes 3-4: 3 minutes.

    All lanes: Western blot - Anti-BRD9 antibody [EPR23888-5] (ab259839) at 1/1000 dilution

    Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Lane 2: HDLM-2 (human hodgkin lymphoma ) whole cell lysate at 20 µg

    Lane 3: LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: Jurkat (human t cell leukemia t lymphocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 67 kDa

    Observed band size: 80 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [EPR23888-5] (ab259839), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [EPR23888-5] (ab259839)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling BRD9 with ab259839 at 1/500 dilution (0.908 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear and weak cytoplasmic staining on human testis. The section was incubated with ab259839 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-BRD9 antibody [EPR23888-5] (ab259839), expandable thumbnail

    Western blot - Anti-BRD9 antibody [EPR23888-5] (ab259839)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID:32457312; 31015438 ).

    Lysates were made freshly and used in WB immediately to minimize protein degradation (lane1-4). Lanes 5-6 are the lysates from same cell lines but have experienced freeze-thaw cycles.

    Exposure time: Lanes 1-4: 37 seconds; Lanes 5-6: 3 minutes.

    All lanes: Western blot - Anti-BRD9 antibody [EPR23888-5] (ab259839) at 1/1000 dilution

    Lanes 1 and 5: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lanes 4 and 6: PC-12 (rat adrenal gland pheochromocytoma ) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 67 kDa

    Observed band size: 80 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [EPR23888-5] (ab259839), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [EPR23888-5] (ab259839)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling BRD9 with ab259839 at 1/500 dilution (0.908 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on mouse testis. The section was incubated with ab259839 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-BRD9 antibody [EPR23888-5] (ab259839), expandable thumbnail

    Western blot - Anti-BRD9 antibody [EPR23888-5] (ab259839)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Lysates used in this blot have experienced freeze-thaw cycles.

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-BRD9 antibody [EPR23888-5] (ab259839) at 1/1000 dilution

    Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 2: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 3: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 67 kDa

    Observed band size: 80 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [EPR23888-5] (ab259839), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [EPR23888-5] (ab259839)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labelling BRD9 with ab259839 at 1/500 dilution (0.908 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear and weak cytoplasmic staining on rat testis. The section was incubated with ab259839 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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Product protocols

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