Rabbit Recombinant Monoclonal BRD9 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human BRD9.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [HL1282] - BSA and Azide free (AB313843), expandable thumbnail](https://content.abcam.com/products/images/brd9-antibody-hl1282-bsa-and-azide-free-ab313843--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img440707.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [HL1282] - BSA and Azide free (AB313843)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [HL1282] - BSA and Azide free (AB313843), expandable thumbnail](https://content.abcam.com/products/images/brd9-antibody-hl1282-bsa-and-azide-free-ab313843--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img440708.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [HL1282] - BSA and Azide free (AB313843)")
![Western blot - Anti-BRD9 antibody [HL1282] - BSA and Azide free (AB313843), expandable thumbnail](https://content.abcam.com/products/images/brd9-antibody-hl1282-bsa-and-azide-free-ab313843--western-blot-img419085.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BRD9 antibody [HL1282] - BSA and Azide free (AB313843)")
![Immunocytochemistry/ Immunofluorescence - Anti-BRD9 antibody [HL1282] - BSA and Azide free (AB313843), expandable thumbnail](https://content.abcam.com/products/images/brd9-antibody-hl1282-bsa-and-azide-free-ab313843--immunocytochemistry-immunofluorescence-img419084.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-BRD9 antibody [HL1282] - BSA and Azide free (AB313843)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human BRD9. Database link Q9H8M2
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Mouse | Expected | Expected | Tested |
| Rat | Expected | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Plays a role in chromatin remodeling and regulation of transcription (PubMed:22464331, PubMed:26365797). Acts as a chromatin reader that recognizes and binds acylated histones: binds histones that are acetylated and/or butyrylated (PubMed:26365797). Component of SWI/SNF chromatin remodeling subcomplex GBAF that carries out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner (PubMed:29374058). Orchestrates also the RAD51-RAD54 complex formation and thereby plays a role in homologous recombination (HR) (PubMed:32457312).
Alternative names
UNQ3040/PRO9856, BRD9, Bromodomain-containing protein 9, Rhabdomyosarcoma antigen MU-RMS-40.8
Recommended products
Rabbit Recombinant Monoclonal BRD9 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human BRD9.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human BRD9. Database link Q9H8M2
- Carrier free
- Yes
- Clone number
- HL1282
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [HL1282] - BSA and Azide free (ab313843), expandable thumbnail](https://content.abcam.com/products/images/brd9-antibody-hl1282-bsa-and-azide-free-ab313843--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img440707.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [HL1282] - BSA and Azide free (ab313843)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [HL1282] - BSA and Azide free (ab313843)
Paraffin-embedded mouse tissue stained for BRD9 protein with ab313843 at a 1/100 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [HL1282] - BSA and Azide free (ab313843), expandable thumbnail](https://content.abcam.com/products/images/brd9-antibody-hl1282-bsa-and-azide-free-ab313843--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img440708.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [HL1282] - BSA and Azide free (ab313843)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [HL1282] - BSA and Azide free (ab313843)
Paraffin-embedded rat tissue stained for BRD9 protein with ab313843 at a 1/100 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
![Western blot - Anti-BRD9 antibody [HL1282] - BSA and Azide free (ab313843), expandable thumbnail](https://content.abcam.com/products/images/brd9-antibody-hl1282-bsa-and-azide-free-ab313843--western-blot-img419085.jpg "Western blot - Anti-BRD9 antibody [HL1282] - BSA and Azide free (ab313843)")
Western blot - Anti-BRD9 antibody [HL1282] - BSA and Azide free (ab313843)
All lanes: Western blot - Anti-BRD9 antibody [HL1282] - BSA and Azide free (ab313843) at 1/1000 dilution
Lane 1: K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell extract at 30 µg
Lane 2: K562 nuclear extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Developed using the ECL technique.
Predicted band size: 67 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-BRD9 antibody [HL1282] - BSA and Azide free (ab313843), expandable thumbnail](https://content.abcam.com/products/images/brd9-antibody-hl1282-bsa-and-azide-free-ab313843--immunocytochemistry-immunofluorescence-img419084.jpg "Immunocytochemistry/ Immunofluorescence - Anti-BRD9 antibody [HL1282] - BSA and Azide free (ab313843)")
Immunocytochemistry/ Immunofluorescence - Anti-BRD9 antibody [HL1282] - BSA and Azide free (ab313843)
Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling BRD9 using ab313843 at a 1/500 dilution (green). Blue: Fluoroshield with DAPI. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1/1000.
Cells were fixed in 4% paraformaldehyde at RT for 15 mins.
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