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AB286194

Anti-BRDT antibody

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Rabbit Polyclonal BRDT antibody. Suitable for WB and reacts with Recombinant fragment - Human samples.

View Alternative Names

Bromodomain testis-specific protein, Cancer/testis antigen 9, RING3-like protein, CT9, BRDT

1 Images
Western blot - Anti-BRDT antibody (AB286194)
  • WB

Supplier Data

Western blot - Anti-BRDT antibody (AB286194)

All lanes:

Western blot - Anti-BRDT antibody (ab286194)

Lane 1:

BRDT (22-138 aa) 2 ng

Lane 2:

BRDT (22-138 aa) 10 ng

Lane 3:

BRDT (22-138 aa) 50 ng

Predicted band size: 108 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200", "WB-species-notes": "<p></p>" } } }
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Product details

This product is manufactured by BioVision, an Abcam company and was previously called 6643 BRDT Antibody. 6643-100 is the same size as the 100 µg size of ab286194.
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.01% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis (PubMed : 22464331, PubMed : 22901802). Required in late pachytene spermatocytes : plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time (PubMed : 22901802). In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA (PubMed : 22901802). Also recognizes and binds a subset of butyrylated histones : able to bind histone H4 butyrylated at 'Lys-8' (H4K8ac), while it is not able to bind H4 butyrylated at 'Lys-5' (H4K5ac) (By similarity). Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids (By similarity). Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.
See full target information BRDT
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Product promise

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