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Rabbit Recombinant Monoclonal BRDT antibody. Suitable for WB, IHC-P and reacts with Recombinant full length protein, Mouse, Rat samples.

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Images

Western blot - Anti-BRDT antibody [EPR25427-24] (AB288435), expandable thumbnail
  • Western blot - Anti-BRDT antibody [EPR25427-24] (AB288435), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRDT antibody [EPR25427-24] (AB288435), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRDT antibody [EPR25427-24] (AB288435), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRDT antibody [EPR25427-24] (AB288435), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PFlow Cyt (Intra)
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Tested
Tested
Not recommended
Rat
Not recommended
Not recommended
Tested
Tested
Not recommended
Recombinant full length protein
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Not recommended
Not recommended

Species
Mouse, Rat, Human, Recombinant full length protein
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Recombinant full length protein
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

-

Species
Rat
Dilution info
1/2000
Notes

-

Not recommended
Not recommended

Species
Human, Recombinant full length protein
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Recombinant full length protein
Dilution info
-
Notes

-

Target data

Function

Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis (PubMed:22464331, PubMed:22901802). Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time (PubMed:22901802). In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA (PubMed:22901802). Also recognizes and binds a subset of butyrylated histones: able to bind histone H4 butyrylated at 'Lys-8' (H4K8ac), while it is not able to bind H4 butyrylated at 'Lys-5' (H4K5ac) (By similarity). Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids (By similarity). Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.

Alternative names

Bromodomain testis-specific protein, Cancer/testis antigen 9, RING3-like protein, CT9, BRDT

Recommended products

Rabbit Recombinant Monoclonal BRDT antibody. Suitable for WB, IHC-P and reacts with Recombinant full length protein, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR25427-24
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-BRDT antibody [EPR25427-24] (ab288435), expandable thumbnail

    Western blot - Anti-BRDT antibody [EPR25427-24] (ab288435)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 59 seconds

    This antibody has no cross-reaction with BRD3 and BRD4.
    All the recombinant proteins are expressed from an E.coli expression system.

    All lanes: Western blot - Anti-BRDT antibody [EPR25427-24] (ab288435) at 1/1000 dilution

    Lane 1: Human BRDT recombinant protein (aa 1-947) 20ng

    Lane 2: Human BRD3 recombinant protein (aa 1-726) 20ng

    Lane 3: Human BRD4 recombinant protein (aa 49-240) 20ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 108 kDa

    Observed band size: 130 kDa

  • Western blot - Anti-BRDT antibody [EPR25427-24] (ab288435), expandable thumbnail

    Western blot - Anti-BRDT antibody [EPR25427-24] (ab288435)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 125 seconds

    Low expression: Kidney, Skeletal muscle
    The observed MW/expression profile is consistent with what has been described in the literature (PMID: 33257688 & Uniprot)

    All lanes: Western blot - Anti-BRDT antibody [EPR25427-24] (ab288435) at 1/1000 dilution

    Lane 1: Mouse testis tissue lysate at 20 µg

    Lane 2: Mouse kidney tissue lysate at 20 µg

    Lane 3: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 4: Rat testis tissue lysate at 20 µg

    Lane 5: Rat kidney tissue lysate at 40 µg

    Lane 6: Rat skeletal muscle tissue lysate at 20 µg

    Secondary

    Lanes 1 - 6: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100000 dilution

    Lanes 1 - 6: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 108 kDa

    Observed band size: 130 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRDT antibody [EPR25427-24] (ab288435), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRDT antibody [EPR25427-24] (ab288435)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling BRDT with ab288435 at 1/2000 (0.268 ug/ml) followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Nuclear staining on germ cells in mouse testis. The section was incubated with ab288435 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™, Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRDT antibody [EPR25427-24] (ab288435), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRDT antibody [EPR25427-24] (ab288435)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labelling BRDT with ab288435 at 1/2000 (0.268 ug/ml) followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Nuclear staining on germ cells in rat testis. The section was incubated with ab288435 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™, Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRDT antibody [EPR25427-24] (ab288435), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRDT antibody [EPR25427-24] (ab288435)

    Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labelling BRDT with ab288435 at 1/2000 (0.268 ug/ml) followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Negative control: no staining on rat skeletal muscle. The section was incubated with ab288435 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™, Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRDT antibody [EPR25427-24] (ab288435), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRDT antibody [EPR25427-24] (ab288435)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labelling BRDT with ab288435 at 1/2000 (0.268 ug/ml) followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Negative control: no staining on mouse kidney. The section was incubated with ab288435 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™, Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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