Anti-BRDT antibody [EPR25427-24] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRDT antibody [EPR25427-24] - BSA and Azide free (AB288442)

This data was developed using ab288435, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labelling BRDT with ab288435 at 1/2000 (0.268 ug/ml) followed by a ready to use Leica DS9800 (Bond™, Polymer Refine Detection) was used. Negative control : no staining on rat skeletal muscle. The section was incubated with ab288435 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRDT antibody [EPR25427-24] - BSA and Azide free (AB288442)

This data was developed using ab288435, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling BRDT with ab288435 at 1/2000 (0.268 ug/ml) followed by a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used. Nuclear staining on germ cells in mouse testis. The section was incubated with ab288435 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™, Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRDT antibody [EPR25427-24] - BSA and Azide free (AB288442)

This data was developed using ab288435, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labelling BRDT with ab288435 at 1/2000 (0.268 ug/ml) followed by a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used. Negative control : no staining on mouse kidney. The section was incubated with ab288435 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRDT antibody [EPR25427-24] - BSA and Azide free (AB288442)

This data was developed using ab288435, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labelling BRDT with ab288435 at 1/2000 (0.268 ug/ml) followed by a ready to use Leica DS9800 (Bond™, Polymer Refine Detection) was used. Nuclear staining on germ cells in rat testis. The section was incubated with ab288435 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• WB

Lab

Western blot - Anti-BRDT antibody [EPR25427-24] - BSA and Azide free (AB288442)

This data was developed using ab288435, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 125 seconds

Low expression : Kidney, Skeletal muscle
The observed MW/expression profile is consistent with what has been described in the literature (PMID : 33257688 & Uniprot)

All lanes:

Western blot - Anti-BRDT antibody [EPR25427-24] (<a href='/en-us/products/primary-antibodies/brdt-antibody-epr25427-24-ab288435'>ab288435</a>) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Lane 3:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 4:

Rat testis tissue lysate at 20 µg

Lane 5:

Rat kidney tissue lysate at 40 µg

Lane 6:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

Lanes 1 - 6:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100000 dilution

Lanes 1 - 6:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 108 kDa

Observed band size: 130 kDa

false

• WB

Supplier Data

Western blot - Anti-BRDT antibody [EPR25427-24] - BSA and Azide free (AB288442)

This data was developed using ab288435, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 59 seconds

This antibody has no cross-reaction with BRD3 and BRD4.
All the recombinant proteins are expressed from an E.coli expression system.

All lanes:

Western blot - Anti-BRDT antibody [EPR25427-24] (<a href='/en-us/products/primary-antibodies/brdt-antibody-epr25427-24-ab288435'>ab288435</a>) at 1/1000 dilution

Lane 1:

Human BRDT recombinant protein (aa 1-947) 20ng

Lane 2:

Human BRD3 recombinant protein (aa 1-726) 20ng

Lane 3:

Human BRD4 recombinant protein (aa 49-240) 20ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 108 kDa

Observed band size: 130 kDa

false