Anti-BrdU antibody (ab152095) is a rabbit polyclonal antibody that is used to detect BrdU in Flow Cytometry, IHC-P, ICC/IF.
- Over 60 publications
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- Journal of translational medicine 21:8522023Paneth cell-derived iNOS is required to maintain homeostasis in the intestinal stem cell niche.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingxiao Huang,Zhenni Xu,Xudan Lei,Yujun Huang,Siyu Tu,Lu Xu,Jieying Xia,Dengqun LiuPubMed 38007452
- Nature communications 14:63412023Subventricular zone cytogenesis provides trophic support for neural repair in a mouse model of stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael R Williamson,Stephanie P Le,Ronald L Franzen,Nicole A Donlan,Jill L Rosow,Mathilda S Nicot-Cartsonis,Alexis Cervantes,Benjamin Deneen,Andrew K Dunn,Theresa A Jones,Michael R DrewPubMed 37816732
- JCI insight 8:2023RBM47 regulates intestinal injury and tumorigenesis by modifying proliferation, oxidative response, and inflammatory pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSaeed Soleymanjahi,Valerie Blanc,Elizabeth A Molitor,David M Alvarado,Yan Xie,Vered Gazit,Jeffrey W Brown,Kathleen Byrnes,Ta-Chiang Liu,Jason C Mills,Matthew A Ciorba,Deborah C Rubin,Nicholas O DavidsonPubMed 37014710
- Cell death & disease 14:122023PLK1 and AURKB phosphorylate survivin differentially to affect proliferation in racially distinct triple-negative breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChakravarthy Garlapati,Shriya Joshi,Shristi Bhattarai,Jayashree Krishnamurthy,Ravi Chakra Turaga,Thi Nguyen,Xiaoxian Li,Ritu AnejaPubMed 36627281
- Neural regeneration research 18:1521-15262022Transient neurogenesis in ischemic cortex from Sox2 astrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia-Lei Yang,Hong Fan,Fan-Fan Fu,Bao-Lin Guo,Ying Huang,Li Sun,Wen-Ting Wang,Jun-Ling Xing,Xin-Tian Hu,Yu-Qiang Ding,Kun Zhang,Ying-Zhou Hu,Ya-Zhou WangPubMed 36571357
- Advanced materials (Deerfield Beach, Fla.) :e22083642022Microparticles Decorated with Cell-Instructive Surface Chemistries Actively Promote Wound Healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArsalan Latif,Leanne E Fisher,Adam A Dundas,Valentina Cuzzucoli Crucitti,Zeynep Imir,Karen Lawler,Francesco Pappalardo,Benjamin W Muir,Ricky Wildman,Derek J Irvine,Morgan R Alexander,Amir M GhaemmaghamiPubMed 36440539
- NPJ Regenerative medicine 7:512022The synovial microenvironment suppresses chondrocyte hypertrophy and promotes articular chondrocyte differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael Chau,Zelong Dou,Marta Baroncelli,Ellie B Landman,Ameya Bendre,Masaru Kanekiyo,Alexandra Gkourogianni,Kevin Barnes,Lars Ottosson,Ola NilssonPubMed 36114234
- Stem cell research & therapy 13:2902022KAP1 phosphorylation promotes the survival of neural stem cells after ischemia/reperfusion by maintaining the stability of PCNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWan Wang,Tianqing Yan,Xinjian Guo,Heng Cai,Chang Liang,Linyan Huang,Yanling Wang,Ping Ma,Suhua QiPubMed 35799276
- Cell reports 39:1108562022DDX41 is required for cGAS-STING activation against DNA virus infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRavi Shankar Singh,Venkatasubramanian Vidhyasagar,Shizhuo Yang,Ananna Bhadra Arna,Manisha Yadav,Aanchal Aggarwal,Alexya N Aguilera,Satoru Shinriki,Kalpana Kalyanasundaram Bhanumathy,Kannupriya Pandey,Aizhang Xu,Noreen Rapin,Mark Bosch,John DeCoteau,Jim Xiang,Franco J Vizeacoumar,Yan Zhou,Vikram Misra,Hirotaka Matsui,Susan R Ross,Yuliang WuPubMed 35613581
- International journal of experimental pathology 103:90-1012022High level of FHL2 exacerbates the outcome of non-small cell lung cancer (NSCLC) patients and the malignant phenotype in NSCLC cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNa Li,Ling Xu,Ji Zhang,Yongyu LiuPubMed 35366027
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Chemical / Small Molecule corresponding to BrdU.
Reactivity data
Select an application| Flow Cyt | IHC-P | ICC/IF | |
|---|---|---|---|
| Chemical | Tested | Tested | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Alternative names
BUdr, Bromodeoxyuridine
Recommended products
Anti-BrdU antibody (ab152095) is a rabbit polyclonal antibody that is used to detect BrdU in Flow Cytometry, IHC-P, ICC/IF.
- Over 60 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Chemical / Small Molecule corresponding to BrdU.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-BrdU antibody (ab152095) is a rabbit polyclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF).
Trusted by the scientific community
Anti-BrdU (ab152095) was first used in a scientific publication in 2012 and has been cited over 60 times in peer-reviewed journals.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody (ab152095)
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells treated with or without 100µM BrdU for 4 hours, labeling BrdU with ab152095 at 1/50 dilution. Lower images stained with Hoechst 33342.
Flow Cytometry - Anti-BrdU antibody (ab152095)
ab152095 detects BrdU protein from HeLa treated with 10 μM BrdU for 30 minutes and fixed in 4% paraformaldehyde at 4°C for 15 minutes by flow cytometry analysis. Black: Unlabelled sample was used as a control. Red: BrdU antibody (ab152095) dilution: 1/50.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody (ab152095)
HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BrdU stained by BrdU antibody (ab152095) diluted at 1/2000. Blue: Hoechst 33342 staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody (ab152095)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse BrdU treated intestine using ab152095 diluted at 1/500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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