Rabbit Recombinant Monoclonal BrdU antibody. Carrier free. Suitable for IHC-P, ICC/IF and reacts with Mouse, Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [HL1111] (AB308341), expandable thumbnail](https://content.abcam.com/products/images/brdu-antibody-hl1111-ab308341--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img407973.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [HL1111] (AB308341)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [HL1111] (AB308341), expandable thumbnail](https://content.abcam.com/products/images/brdu-antibody-hl1111-ab308341--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img407969.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [HL1111] (AB308341)")
![Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [HL1111] (AB308341), expandable thumbnail](https://content.abcam.com/products/images/brdu-antibody-hl1111-ab308341--immunocytochemistry-immunofluorescence-img407968.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [HL1111] (AB308341)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Alternative names
BUdr, Bromodeoxyuridine
Rabbit Recombinant Monoclonal BrdU antibody. Carrier free. Suitable for IHC-P, ICC/IF and reacts with Mouse, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Carrier free
- Yes
- Clone number
- HL1111
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [HL1111] (ab308341), expandable thumbnail](https://content.abcam.com/products/images/brdu-antibody-hl1111-ab308341--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img407973.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [HL1111] (ab308341)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [HL1111] (ab308341)
For immunofluorescence analysis, mock and treated HeLa cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of BrdU using ab308341 at 1/2000 dilution (green). Blue: Fluoroshield with DAPI
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [HL1111] (ab308341), expandable thumbnail](https://content.abcam.com/products/images/brdu-antibody-hl1111-ab308341--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img407969.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [HL1111] (ab308341)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [HL1111] (ab308341)
Immunohistochemical analysis of mouse intestinal tissue pulsed with BrdU tissue labeling BrdU using ab308341 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min
![Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [HL1111] (ab308341), expandable thumbnail](https://content.abcam.com/products/images/brdu-antibody-hl1111-ab308341--immunocytochemistry-immunofluorescence-img407968.jpg "Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [HL1111] (ab308341)")
Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [HL1111] (ab308341)
For immunofluorescence analysis, mock and treated HeLa cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of BrdU using ab308341 at 1/2000 dilution (green). Blue: Fluoroshield with DAPI.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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