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Anti-BrdU antibody [IIB5] (ab8152) is a mouse monoclonal antibody detecting BrdU in Flow Cytometry, IHC-P, IHC-Fr, ICC/IF.



- Over 80 publications

- Trusted since 2002



Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (AB8152), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (AB8152), expandable thumbnail
  • Flow Cytometry - Anti-BrdU antibody [IIB5] (AB8152), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Chemical / Small Molecule corresponding to BrdU.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytIHC-FoFrIHC-PICC/IFIHC-Fr
Chemical
Tested
Expected
Tested
Tested
Expected

Tested
Tested

Species
Chemical
Dilution info
1/100.00000 - 1/200.00000
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Chemical
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Chemical
Dilution info
1/5.00000 - 1/10.00000
Notes

Enzymatic antigen retrieval with proteases can also be used.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Chemical
Dilution info
-
Notes

-

Expected
Expected

Species
Chemical
Dilution info
1/5.00000 - 1/10.00000
Notes

Dilute antibody in 0.15 M phosphate buffered saline with 1% BSA and 1% Na-azide.

Associated Products

Select an associated product type

1 product for Alternative Version

8 products for Alternative Product

Alternative names

Recommended products

Anti-BrdU antibody [IIB5] (ab8152) is a mouse monoclonal antibody detecting BrdU in Flow Cytometry, IHC-P, IHC-Fr, ICC/IF.



- Over 80 publications

- Trusted since 2002


Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Chemical / Small Molecule corresponding to BrdU.
Clone number
IIB5
Purity
Tissue culture supernatant
Specificity

BrdU is a thymidine analogue and when offered to proliferating cells it is incroporated into reduplicating cells. The antibody is specific for DNA in which BrdU has been incorporated. In immunoassays this antibody reacts strongly with free or carrier-protein coupled BrdU but not with other nucleosides. In immuncytochemistry the antibody only recognizes BrdU in denaturated (single stranded) DNA. The BrdU antibody is 100% crossreactive with Iodo-Deoxy-Uridine (IrdU). Therfore, IdU instead of BrdU can be used in studies.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Notes

What is this antibody validated in?


Anti-BrdU antibody [IIB5] (ab8152) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF).

Trusted by the scientific community


Anti-BrdU [IIB5] (ab8152) was first used in a scientific publication in 2002 and has been cited over 80 times in peer-reviewed journals.

Reviewed by scientists


Anti-BrdU [IIB5] (ab8152) has over 5 independent reviews from customers.

Other related products


We have a range of other formats of antibody clone [IIB5] also available for your convenience:
ab8152, Anti-BrdU antibody [IIB5] ab8955



Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8152), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8152)

    Immunohistochemical analysis of rat brain tissue, staining BrdU with ab8152.

    Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/20 in diluent) for 18 hours at 25°C. A Cy3®-conjugated donkey anti-mouse polyclonal IgG was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8152), expandable thumbnail
    Image courtesy of a customer review submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada

    Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8152)

    ab8152 (1/100) staining BrdU in HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further esperimental details please see Abreview.

  • Flow Cytometry - Anti-BrdU antibody [IIB5] (ab8152), expandable thumbnail

    Flow Cytometry - Anti-BrdU antibody [IIB5] (ab8152)

    Cells were pulse labeled with 10 μM BrdU for 30 min, rinsed twice in prewarmed PBS, and chased in prewarmed culture medium, supplemented with 5 mM deoxythymidine. Incorporated BrdU was detected after ethanol fixation of the cells, which were than rinsed once in PBS and resuspended in 2 ml of 0.4 mg/ml pepsin in 0.1 N HCl. After 30 min at room temperature cells were pelleted, resuspended in 2 N HCl, and incubated for another 30 min at 37°C. Cells were rinsed in 0.1 M borate buffer, pH 8.5, and PBS/BSA (1 mg/ml BSA in PBS). Appropriately diluted mouse anti-BrdU antibody (clone IIB5) was added to the cell pellet, resuspended in 100 micro liters PBS/BSA. After incubation for 1 h at room temperature, the cells were rinsed twice in PBS/BSA. For visualization, FITC-conjugated Fab2 fragments of rabbit anti-mouse Ig antibody were added in a 1/10 dilution. After incubation for 45 min at room temperature samples were rinsed twice in PBS/BSA and the cells were finally resuspended in 0.5 ml cold PBS supplemented with 100 microgram/ml RNAse and 20 μg/mL propidium iodide. The samples were allowed to stand for 15 min on ice in the dark before flow cytometric analysis. In the negative control the primary antibody was omitted.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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