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AB8955

Anti-BrdU antibody [IIB5]

4

(3 Reviews)

|

(38 Publications)

Anti-BrdU antibody [IIB5] (ab8955) is a mouse monoclonal antibody detecting BrdU in Flow Cytometry, IHC-P, IHC-Fr, ICC/IF.

- Over 30 publications
- Trusted since 2002

View Alternative Names

BUdr, Bromodeoxyuridine

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (AB8955)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (AB8955)

Indirect immunofluorescence staining of BrdU-labeled MR65 lung cancer cells using ab8955 (1 : 1000).

Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (AB8955)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (AB8955)

Indirect immunofluorescence staining of BrdU-labeled MR65 lung cancer cells with ab8955.

Flow Cytometry - Anti-BrdU antibody [IIB5] (AB8955)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-BrdU antibody [IIB5] (AB8955)

Flow cytometric BrdU-DNA cell cycle analysis in a lung cancer cell line. Dilution 1 : 100 of primary antibody ab8955. FITC-conjugated goat anti mouse secondary antibody was used for detection.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (AB8955)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (AB8955)

BrdU staining in paraffin embedded rat intestine using ab8955. Primary antibody diluted 1 : 200. Peroxidase-conjugated goat anti mouse secondary antibody was used for detection.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (AB8955)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (AB8955)

BrdU staining in sponge Halisarca caerulea using ab8955. Metacrylate embedded tissue sections were incubated with 1 : 200 diluted primary antibody. An avidin-biotin complex kit using biotinylated rabbit anti-mouse antibody and then incubation in avidin-biotin-peroxidase complex was used for detection.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

IIB5

Isotype

IgG1

Carrier free

No

Applications

ICC/IF, IHC-P, Flow Cyt, IHC-Fr

applications

Specificity

This antibody also reacts with bromodeoxyuridine when incorporated into nuclear DNA. It cross reacts with iododeoxyuridine (IdU).

Reactivity data

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Product details

What is this antibody validated in?
Anti-BrdU antibody [IIB5] (ab8955) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF). Trusted by the scientific community
Anti-BrdU [IIB5] (ab8955) was first used in a scientific publication in 2002 and has been cited over 30 times in peer-reviewed journals.

Other related products
We have a range of other formats of antibody clone [IIB5] also available for your convenience: ab8152, ab8955

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (38)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 22:653 PubMed39443923

2024

Curcumin/pEGCG-encapsulated nanoparticles enhance spinal cord injury recovery by regulating CD74 to alleviate oxidative stress and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Tianjun Chen,Li Wan,Yongchun Xiao,Ke Wang,Ping Wu,Can Li,Caiqiang Huang,Xiangge Liu,Wei Xue,Guodong Sun,Xin Ji,Hongsheng Lin,Zhisheng Ji

International journal of molecular sciences 25: PubMed38928267

2024

Arginine Vasotocin Directly Regulates Spermatogenesis in Adult Zebrafish () Testes.

Applications

Unspecified application

Species

Unspecified reactive species

Maya Zanardini,Weimin Zhang,Hamid R Habibi

Chemical biology & drug design 103:e14429 PubMed38230769

2024

Stachydrine represses the proliferation and enhances cell cycle arrest and apoptosis of breast cancer cells via PLA2G2A/DCN axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Zhai,Tianlong Mu,Lina Zhao,Dongsheng Zhu,Xin Zhong,Yiliang Li,Chen Liang,Wei Li,Qingyuan Zhou

Biological research 56:65 PubMed38041203

2023

Electroacupuncture promotes neurogenesis in the dentate gyrus and improves pattern separation in an early Alzheimer's disease mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Yanyi Ding,Long Li,Sinuo Wang,Yajun Cao,Minguang Yang,Yaling Dai,Huawei Lin,Jianhong Li,Yulu Liu,Zhifu Wang,Weilin Liu,Jing Tao

Nature cell biology 25:1833-1847 PubMed37945904

2023

MAF amplification licenses ERα through epigenetic remodelling to drive breast cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Alicia Llorente,María Teresa Blasco,Irene Espuny,Marc Guiu,Cecilia Ballaré,Enrique Blanco,Adrià Caballé,Anna Bellmunt,Fernando Salvador,Andrea Morales,Marc Nuñez,Guillem Loren,Francesca Imbastari,Marta Fidalgo,Cristina Figueras-Puig,Patrizia Gibler,Mariona Graupera,Freddy Monteiro,Antoni Riera,Ingunn Holen,Alexandra Avgustinova,Luciano Di Croce,Roger R Gomis

Brain sciences 13: PubMed36979321

2023

Minocycline Attenuates Sevoflurane-Induced Postoperative Cognitive Dysfunction in Aged Mice by Suppressing Hippocampal Apoptosis and the Notch Signaling Pathway-Mediated Neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Liang,Shanshan Han,Chao Ye,Haimeng Zhu,Jiajun Wu,Yunjuan Nie,Gaoshang Chai,Peng Zhao,Dengxin Zhang

EMBO reports 24:e54977 PubMed36416237

2022

NONO enhances mRNA processing of super-enhancer-associated GATA2 and HAND2 genes in neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Song Zhang,Jack Al Cooper,Yee Seng Chong,Alina Naveed,Chelsea Mayoh,Nisitha Jayatilleke,Tao Liu,Sebastian Amos,Simon Kobelke,Andrew C Marshall,Oliver Meers,Yu Suk Choi,Charles S Bond,Archa H Fox

PLoS pathogens 18:e1010839 PubMed36137145

2022

Knockout of liver fluke granulin, Ov-grn-1, impedes malignant transformation during chronic infection with Opisthorchis viverrini.

Applications

Unspecified application

Species

Unspecified reactive species

Sujittra Chaiyadet,Sirikachorn Tangkawattana,Michael J Smout,Wannaporn Ittiprasert,Victoria H Mann,Raksawan Deenonpoe,Patpicha Arunsan,Alex Loukas,Paul J Brindley,Thewarach Laha

Molecular neurobiology 59:6613-6631 PubMed35974295

2022

AIF Overexpression Aggravates Oxidative Stress in Neonatal Male Mice After Hypoxia-Ischemia Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Li,Yanyan Sun,Shan Zhang,Yiran Xu,Kenan Li,Cuicui Xie,Yong Wang,Yafeng Wang,Jing Cao,Xiaoyang Wang,Josef M Penninger,Guido Kroemer,Klas Blomgren,Changlian Zhu

Nature communications 13:3190 PubMed35680905

2022

SMYD5 catalyzes histone H3 lysine 36 trimethylation at promoters.

Applications

Unspecified application

Species

Unspecified reactive species

Yanjun Zhang,Yuan Fang,Yin Tang,Shixun Han,Junqi Jia,Xinyi Wan,Jiaqi Chen,Ying Yuan,Bin Zhao,Dong Fang
View all publications

Product promise

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