Anti-BrdU antibody [IIB5] (ab8955) is a mouse monoclonal antibody that is used to detect BrdU in Flow Cytometry, IHC-P, IHC-Fr, ICC/IF.
- Over 30 publications
- Trusted since 2002
Images
![Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (AB8955), expandable thumbnail](https://content.abcam.com/products/images/brdu-antibody-iib5-ab8955--immunocytochemistry-immunofluorescence-img3192.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (AB8955)")
![Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (AB8955), expandable thumbnail](https://content.abcam.com/products/images/brdu-antibody-iib5-ab8955--immunocytochemistry-immunofluorescence-img121929.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (AB8955)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (AB8955), expandable thumbnail](https://content.abcam.com/products/images/brdu-antibody-iib5-ab8955--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77334.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (AB8955)")
![Flow Cytometry - Anti-BrdU antibody [IIB5] (AB8955), expandable thumbnail](https://content.abcam.com/products/images/brdu-antibody-iib5-ab8955--flow-cytometry-img104508.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-BrdU antibody [IIB5] (AB8955)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (AB8955), expandable thumbnail](https://content.abcam.com/products/images/brdu-antibody-iib5-ab8955--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126615.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (AB8955)")
Publications
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- Biological research 56:652023Electroacupuncture promotes neurogenesis in the dentate gyrus and improves pattern separation in an early Alzheimer's disease mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanyi Ding,Long Li,Sinuo Wang,Yajun Cao,Minguang Yang,Yaling Dai,Huawei Lin,Jianhong Li,Yulu Liu,Zhifu Wang,Weilin Liu,Jing TaoPubMed 38041203
- Nature cell biology 25:1833-18472023MAF amplification licenses ERα through epigenetic remodelling to drive breast cancer metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlicia Llorente,María Teresa Blasco,Irene Espuny,Marc Guiu,Cecilia Ballaré,Enrique Blanco,Adrià Caballé,Anna Bellmunt,Fernando Salvador,Andrea Morales,Marc Nuñez,Guillem Loren,Francesca Imbastari,Marta Fidalgo,Cristina Figueras-Puig,Patrizia Gibler,Mariona Graupera,Freddy Monteiro,Antoni Riera,Ingunn Holen,Alexandra Avgustinova,Luciano Di Croce,Roger R GomisPubMed 37945904
- Brain sciences 13:2023Minocycline Attenuates Sevoflurane-Induced Postoperative Cognitive Dysfunction in Aged Mice by Suppressing Hippocampal Apoptosis and the Notch Signaling Pathway-Mediated Neuroinflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunjie Liang,Shanshan Han,Chao Ye,Haimeng Zhu,Jiajun Wu,Yunjuan Nie,Gaoshang Chai,Peng Zhao,Dengxin ZhangPubMed 36979321
- eLife 10:2021FMRP regulates mRNAs encoding distinct functions in the cell body and dendrites of CA1 pyramidal neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCaryn R Hale,Kirsty Sawicka,Kevin Mora,John J Fak,Jin Joo Kang,Paula Cutrim,Katarzyna Cialowicz,Thomas S Carroll,Robert B DarnellPubMed 34939924
- eLife 10:2021Augmin deficiency in neural stem cells causes p53-dependent apoptosis and aborts brain development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRicardo Viais,Marcos Fariña-Mosquera,Marina Villamor-Payà,Sadanori Watanabe,Lluís Palenzuela,Cristina Lacasa,Jens LüdersPubMed 34427181
- Genes & development 35:771-7812021AGO HITS-CLIP reveals distinct miRNA regulation of white and brown adipose tissue identity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSean O'Connor,Elisabeth A Murphy,Sarah K Szwed,Matt Kanke,François Marchildon,Praveen Sethupathy,Robert B Darnell,Paul CohenPubMed 33832988
- Cell host & microbe 29:834-848.e132021Argonaute-CLIP delineates versatile, functional RNAi networks in Aedes aegypti, a major vector of human viruses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKathryn Rozen-Gagnon,Meigang Gu,Joseph M Luna,Ji-Dung Luo,Soon Yi,Sasha Novack,Eliana Jacobson,Wei Wang,Matthew R Paul,Troels K H Scheel,Thomas Carroll,Charles M RicePubMed 33794184
- eLife 9:2020Ribosomal profiling during prion disease uncovers progressive translational derangement in glia but not in neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClaudia Scheckel et. al.PubMed 32960170
- Cellular and molecular life sciences : CMLS 78:2213-22302020NEAT1 polyA-modulating antisense oligonucleotides reveal opposing functions for both long non-coding RNA isoforms in neuroblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlina Naveed,Jack A Cooper,Ruohan Li,Alysia Hubbard,Jingwei Chen,Tao Liu,Steve D Wilton,Sue Fletcher,Archa H FoxPubMed 32914209
- Oncology reports 43:1169-11862020MicroRNA‑22 mediates the cisplatin resistance of osteosarcoma cells by inhibiting autophagy via the PI3K/Akt/mTOR pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen-Yang Meng et. al.PubMed 32323781
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Chemical / Small Molecule corresponding to BrdU.
Reactivity data
Select an application| Flow Cyt | IHC-P | ICC/IF | IHC-Fr | |
|---|---|---|---|---|
| Chemical | Tested | Tested | Tested | Expected |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info 1/100.00000 - 1/200.00000 | Notes ABC method. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes Using immunocytochemistry, a combination of this antibody and Mouse monoclonal to Mitotic Cells (ab8956) can distinguish and quantitate the four major fractions of the cell cycle. |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info 1/100.00000 - 1/200.00000 | Notes ABC method. |
Associated Products
Select an associated product type
1 product for Alternative Version
8 products for Alternative Product
Target data
Alternative names
BUdr, Bromodeoxyuridine
Recommended products
Anti-BrdU antibody [IIB5] (ab8955) is a mouse monoclonal antibody that is used to detect BrdU in Flow Cytometry, IHC-P, IHC-Fr, ICC/IF.
- Over 30 publications
- Trusted since 2002
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Chemical / Small Molecule corresponding to BrdU.
- Clone number
- IIB5
- Purification technique
- Affinity purification Protein G
- Specificity
This antibody also reacts with bromodeoxyuridine when incorporated into nuclear DNA. It cross reacts with iododeoxyuridine (IdU).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-BrdU antibody [IIB5] (ab8955) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF).
Trusted by the scientific community
Anti-BrdU [IIB5] (ab8955) was first used in a scientific publication in 2002 and has been cited over 30 times in peer-reviewed journals.
Other related products
We have a range of other formats of antibody clone [IIB5] also available for your convenience:
Anti-BrdU antibody [IIB5] ab8152, ab8955
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955), expandable thumbnail](https://content.abcam.com/products/images/brdu-antibody-iib5-ab8955--immunocytochemistry-immunofluorescence-img3192.jpg "Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)")
Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)
Indirect immunofluorescence staining of BrdU-labeled MR65 lung cancer cells using ab8955 (1:1000).
![Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955), expandable thumbnail](https://content.abcam.com/products/images/brdu-antibody-iib5-ab8955--immunocytochemistry-immunofluorescence-img121929.jpg "Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)")
Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)
Indirect immunofluorescence staining of BrdU-labeled MR65 lung cancer cells with ab8955.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955), expandable thumbnail](https://content.abcam.com/products/images/brdu-antibody-iib5-ab8955--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77334.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955)
BrdU staining in sponge Halisarca caerulea using ab8955. Metacrylate embedded tissue sections were incubated with 1:200 diluted primary antibody. An avidin-biotin complex kit using biotinylated rabbit anti-mouse antibody and then incubation in avidin-biotin-peroxidase complex was used for detection.
![Flow Cytometry - Anti-BrdU antibody [IIB5] (ab8955), expandable thumbnail](https://content.abcam.com/products/images/brdu-antibody-iib5-ab8955--flow-cytometry-img104508.jpg "Flow Cytometry - Anti-BrdU antibody [IIB5] (ab8955)")
Flow Cytometry - Anti-BrdU antibody [IIB5] (ab8955)
Flow cytometric BrdU-DNA cell cycle analysis in a lung cancer cell line. Dilution 1:100 of primary antibody ab8955. FITC-conjugated goat anti mouse secondary antibody was used for detection.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955), expandable thumbnail](https://content.abcam.com/products/images/brdu-antibody-iib5-ab8955--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126615.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955)
BrdU staining in paraffin embedded rat intestine using ab8955. Primary antibody diluted 1:200. Peroxidase-conjugated goat anti mouse secondary antibody was used for detection.
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