AB111719

Anti-Brevican antibody

Name: Anti-Brevican antibody (ab111719) | Abcam
Brand: Abcam Limited
SKU: AB111719
Price: 490 USD
Availability: InStock

(1 Review)

(6 Publications)

Rabbit Polyclonal Brevican antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BCAN aa 50-300.

View Alternative Names

BEHAB, CSPG7, UNQ2525/PRO6018, BCAN, Brevican core protein, Brain-enriched hyaluronan-binding protein, Chondroitin sulfate proteoglycan 7

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brevican antibody (AB111719)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brevican antibody (AB111719)

ab111719 at 1/500 dilution staining Brevican in Formalin-fixed, paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry.

Unknown

Western blot - Anti-Brevican antibody (AB111719)

7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Brevican antibody (ab111719) at 1/500 dilution

All lanes:

MCF-7 whole cell lysate at 30 µg

Predicted band size: 99 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human BCAN aa 50-300. The exact immunogen used to generate this antibody is proprietary information.

Q96GW7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab111719 is purified by antigen-affinity chromatography.

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Brevican also known as BEHAB is a prominent extracellular matrix proteoglycan. This molecule with a molecular weight of approximately 145 kDa mainly expresses in the central nervous system. It is found especially in the brain and contributes significantly to neural network organization. Brevican is a major component of perineuronal nets specialized structures that surround neurons and support their functions.

Biological function summary

Brevican regulates cell adhesion migration and axonal guidance. As part of a proteoglycan complex it interacts with other matrix components and cell surface receptors to influence cell signaling. Its modular structure allows it to bind with various molecules which can modulate extracellular matrix composition and neuronal connectivity. This regulation is vital for maintaining synaptic plasticity and brain tissue integrity.

Pathways

Brevican plays an important role in neural development and plasticity. It participates in the extracellular matrix pathway that modulates neuronal-circuit dynamics. This pathway involves interactions with molecules like versican and neurocan which are integral to synaptic stabilization and remodeling. Brevican's interactions within these pathways help coordinate extracellular and intracellular processes that underlie neural network adaptations.

Brevican has strong associations with brain tumors and neurodegenerative diseases. Research indicates its altered expression in gliomas where it may influence tumor growth and cell invasion. Changes in brevican levels also link to Alzheimer's disease where it might affect amyloid plaque formation. In these conditions brevican's interactions with proteins such as tenascin-R and chondroitin sulfate proteoglycans could impact disease progression and cellular responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role in the terminally differentiating and the adult nervous system during postnatal development. Could stabilize interactions between hyaluronan (HA) and brain proteoglycans.

See full target information BCAN

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Biomedical reports 20:7 PubMed38124768

2023

Prolonged use of temozolomide leads to increased anxiety and decreased content of aggrecan and chondroitin sulfate in brain tissues of aged rats.

Applications

Unspecified application

Species

Unspecified reactive species

Anastasia V Strokotova,Dmitry K Sokolov,Olga P Molodykh,Elena V Koldysheva,Evgenii E Kliver,Victor S Ushakov,Maxim O Politko,Nadezhda V Mikhnevich,Galina M Kazanskaya,Svetlana V Aidagulova,Elvira V Grigorieva

Proceedings of the National Academy of Sciences of the United States of America 119: PubMed35217608

2022

Landscape of surfaceome and endocytome in human glioma is divergent and depends on cellular spatial organization.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Governa,Hugo Talbot,Kelin Gonçalves de Oliveira,Myriam Cerezo-Magaña,Anna Bång-Rudenstam,Maria C Johansson,Ann-Sofie Månsson,Karin Forsberg-Nilsson,György Marko-Varga,Julio Enríquez Pérez,Anna Darabi,Johan Malmström,Johan Bengzon,Charlotte Welinder,Mattias Belting

International journal of molecular sciences 22: PubMed34948147

2021

Multiple Irradiation Affects Cellular and Extracellular Components of the Mouse Brain Tissue and Adhesion and Proliferation of Glioblastoma Cells in Experimental System In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Maxim O Politko,Alexandra Y Tsidulko,Oxana A Pashkovskaya,Konstantin E Kuper,Anastasia V Suhovskih,Galina M Kazanskaya,Lyubov S Klyushova,Dmitry K Sokolov,Alexander M Volkov,Evgenii E Kliver,Alexander A Zheravin,Svetlana V Aidagulova,Elvira V Grigorieva

BMC neuroscience 21:16 PubMed32334536

2020

Noise exposure alters MMP9 and brevican expression in the rat primary auditory cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Sung-Su Park,Da-Hye Lee,So Min Lee,Chang Ho Lee,So Young Kim

Acta biomaterialia 74:207-221 PubMed29777959

2018

Extracellular matrix component expression in human pluripotent stem cell-derived retinal organoids recapitulates retinogenesis in vivo and reveals an important role for IMPG1 and CD44 in the development of photoreceptors and interphotoreceptor matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Majed Felemban,Birthe Dorgau,Nicola Claire Hunt,Dean Hallam,Darin Zerti,Roman Bauer,Yuchun Ding,Joseph Collin,David Steel,Natalio Krasnogor,Jumana Al-Aama,Susan Lindsay,Carla Mellough,Majlinda Lako

The Journal of neuroscience : the official journal of the Society for Neuroscience 32:18009-17, 18017a PubMed23238717

2012

Perisynaptic chondroitin sulfate proteoglycans restrict structural plasticity in an integrin-dependent manner.

Applications

ICC/IF

Species

Mouse

Clara Orlando,Jeanne Ster,Urs Gerber,James W Fawcett,Olivier Raineteau

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Brevican antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brevican antibody (AB111719)

Western blot - Anti-Brevican antibody (AB111719)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise