Rabbit Recombinant Monoclonal Brevican antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-Brevican antibody [EPR25743-25] - BSA and Azide free (Capture) (AB290662), expandable thumbnail](https://content.abcam.com/products/images/brevican-antibody-epr25743-25-bsa-and-azide-free-capture-ab290662--sandwich-elisa-img178101.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Brevican antibody [EPR25743-25] - BSA and Azide free (Capture) (AB290662)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
| Mouse | Not recommended |
| Rat | Not recommended |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
May play a role in the terminally differentiating and the adult nervous system during postnatal development. Could stabilize interactions between hyaluronan (HA) and brain proteoglycans.
Alternative names
BEHAB, CSPG7, UNQ2525/PRO6018, BCAN, Brevican core protein, Brain-enriched hyaluronan-binding protein, Chondroitin sulfate proteoglycan 7
Recommended products
Rabbit Recombinant Monoclonal Brevican antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25743-25
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab290662 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Brevican. The recommended pair for sandwich ELISA is:
Capture: ab290662 , Human Brevican Capture Antibody (unconjugated)
Detector: Anti-Brevican antibody [EPR25743-72] - BSA and Azide free (Detector) ab290663 , Human Brevican Detector Antibody (unconjugated)
The reference range value is 7.8 - 500 pg/mL.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Brevican also known as BEHAB is a prominent extracellular matrix proteoglycan. This molecule with a molecular weight of approximately 145 kDa mainly expresses in the central nervous system. It is found especially in the brain and contributes significantly to neural network organization. Brevican is a major component of perineuronal nets specialized structures that surround neurons and support their functions.
- Biological function summary
Brevican regulates cell adhesion migration and axonal guidance. As part of a proteoglycan complex it interacts with other matrix components and cell surface receptors to influence cell signaling. Its modular structure allows it to bind with various molecules which can modulate extracellular matrix composition and neuronal connectivity. This regulation is vital for maintaining synaptic plasticity and brain tissue integrity.
- Pathways
Brevican plays an important role in neural development and plasticity. It participates in the extracellular matrix pathway that modulates neuronal-circuit dynamics. This pathway involves interactions with molecules like versican and neurocan which are integral to synaptic stabilization and remodeling. Brevican's interactions within these pathways help coordinate extracellular and intracellular processes that underlie neural network adaptations.
- Associated diseases and disorders
Brevican has strong associations with brain tumors and neurodegenerative diseases. Research indicates its altered expression in gliomas where it may influence tumor growth and cell invasion. Changes in brevican levels also link to Alzheimer's disease where it might affect amyloid plaque formation. In these conditions brevican's interactions with proteins such as tenascin-R and chondroitin sulfate proteoglycans could impact disease progression and cellular responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-Brevican antibody [EPR25743-25] - BSA and Azide free (Capture) (ab290662), expandable thumbnail](https://content.abcam.com/products/images/brevican-antibody-epr25743-25-bsa-and-azide-free-capture-ab290662--sandwich-elisa-img178101.jpg "Sandwich ELISA - Anti-Brevican antibody [EPR25743-25] - BSA and Azide free (Capture) (ab290662)")
Sandwich ELISA - Anti-Brevican antibody [EPR25743-25] - BSA and Azide free (Capture) (ab290662)
Sandwich ELISA of Human Brevican Antibody Pair - BSA and Azide free ab290696 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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