Anti-Brevican antibody [EPR25743-59] (ab285162)

Rabbit Recombinant Monoclonal Brevican antibody. Suitable for IP, WB, IHC-P, IHC-Fr and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

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Images

Immunoprecipitation - Anti-Brevican antibody [EPR25743-59] (AB285162), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	IP	WB	IHC-P	IHC-Fr	Flow Cyt (Intra)
Human	Not recommended	Tested	Tested	Tested	Expected	Not recommended
Mouse	Not recommended	Tested	Tested	Tested	Expected	Not recommended
Rat	Not recommended	Expected	Tested	Tested	Tested	Not recommended

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes -
Species Human	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/2000	Notes -
Species Rat	Dilution info 1/2000	Notes -
Species Human	Dilution info 1/2000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Target data

See full target information BCAN

Function

May play a role in the terminally differentiating and the adult nervous system during postnatal development. Could stabilize interactions between hyaluronan (HA) and brain proteoglycans.

Alternative names

BEHAB, CSPG7, UNQ2525/PRO6018, BCAN, Brevican core protein, Brain-enriched hyaluronan-binding protein, Chondroitin sulfate proteoglycan 7

Recommended products

Rabbit Recombinant Monoclonal Brevican antibody. Suitable for IP, WB, IHC-P, IHC-Fr and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR25743-59
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Brevican also known as BEHAB is a prominent extracellular matrix proteoglycan. This molecule with a molecular weight of approximately 145 kDa mainly expresses in the central nervous system. It is found especially in the brain and contributes significantly to neural network organization. Brevican is a major component of perineuronal nets specialized structures that surround neurons and support their functions.

Biological function summary

Brevican regulates cell adhesion migration and axonal guidance. As part of a proteoglycan complex it interacts with other matrix components and cell surface receptors to influence cell signaling. Its modular structure allows it to bind with various molecules which can modulate extracellular matrix composition and neuronal connectivity. This regulation is vital for maintaining synaptic plasticity and brain tissue integrity.

Pathways

Brevican plays an important role in neural development and plasticity. It participates in the extracellular matrix pathway that modulates neuronal-circuit dynamics. This pathway involves interactions with molecules like versican and neurocan which are integral to synaptic stabilization and remodeling. Brevican's interactions within these pathways help coordinate extracellular and intracellular processes that underlie neural network adaptations.

Associated diseases and disorders

Brevican has strong associations with brain tumors and neurodegenerative diseases. Research indicates its altered expression in gliomas where it may influence tumor growth and cell invasion. Changes in brevican levels also link to Alzheimer's disease where it might affect amyloid plaque formation. In these conditions brevican's interactions with proteins such as tenascin-R and chondroitin sulfate proteoglycans could impact disease progression and cellular responses.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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13 product images

Immunoprecipitation - Anti-Brevican antibody [EPR25743-59] (ab285162)
Brevican was immunoprecipitated from 0.35 mg Human hypothalamus tissue lysate 10 ug with ab285162 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab285162 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: Human hypothalamus tissue lysate 10 ug
Lane 2: ab285162 IP in Human hypothalamus tissue lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab285162 in Human hypothalamus tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 119 seconds
This blot was developed using a higher sensitivity ECL substrate.
All lanes: Immunoprecipitation - Anti-Brevican antibody [EPR25743-59] (ab285162)
Predicted band size: 99 kDa
Western blot - Anti-Brevican antibody [EPR25743-59] (ab285162)
Blocking and diluting buffer and concentration: 5% NFDM/TBST150-kDa full length and 55-kDa secreted isoform are observed. The MW observed is consistent with what has been described in the literature (PMID: 32559242, PMID: 10801887).
Bands below 55-kDa may due to degradation.
Negative control: kidney (PMID: 32559242).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 136 seconds
All lanes: Western blot - Anti-Brevican antibody [EPR25743-59] (ab285162) at 1/1000 dilution
Lane 1: Human hypothalamus tissue lysate at 20 µg
Lane 2: Human kidney tissue lysate at 20 µg
Secondary
All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 99 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brevican antibody [EPR25743-59] (ab285162)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling Brevican with ab285162 at 1/2000 (0.295 ug/ml) followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on human cerebrum. The section was incubated with ab285162 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunoprecipitation - Anti-Brevican antibody [EPR25743-59] (ab285162)
Brevican was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab285162 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab285162 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: Mouse brain tissue lysate 10 ug
Lane 2: ab285162 IP in Mouse brain tissue lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab285162 in mouse brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds
All lanes: Immunoprecipitation - Anti-Brevican antibody [EPR25743-59] (ab285162)
Predicted band size: 99 kDa
Western blot - Anti-Brevican antibody [EPR25743-59] (ab285162)
Blocking and diluting buffer and concentration: 5% NFDM/TBST150-kDa full length and 55-kDa secreted isoform are observed. The MW observed is consistent with what has been described in the literature (PMID: 32559242, PMID: 10801887).
Bands below 55-kDa may due to degradation.
Negative control: heart, kidney, spleen (PMID: 32559242).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes
All lanes: Western blot - Anti-Brevican antibody [EPR25743-59] (ab285162) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse heart tissue lysate at 20 µg
Lane 3: Mouse kidney tissue lysate at 20 µg
Lane 4: Mouse spleen tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 99 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brevican antibody [EPR25743-59] (ab285162)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling Brevican with ab285162 at 1/2000 (0.295 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ , Polymer Refine Detection) was used. Positive staining on mouse cerebrum. The section was incubated with ab285162 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ , Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Frozen sections) - Anti-Brevican antibody [EPR25743-59] (ab285162)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling Brevican with ab285162 at 1/100 (19.72 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 μg/ml) dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/ml) dilution.
.
Western blot - Anti-Brevican antibody [EPR25743-59] (ab285162)
Blocking and diluting buffer and concentration: 5% NFDM/TBST150-kDa full length and 55-kDa secreted isoform are observed. The MW observed is consistent with what has been described in the literature (PMID: 32559242, PMID: 10801887).
Bands below 55-kDa may due to degradation.
Negative control: heart, spleen (PMID: 32559242).
Exposure time: 3 minutes
All lanes: Western blot - Anti-Brevican antibody [EPR25743-59] (ab285162) at 1/1000 dilution
Lane 1: Rat brain tissue lysate at 20 µg
Lane 2: Rat heart tissue lysate at 20 µg
Lane 3: Rat spleen tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 99 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brevican antibody [EPR25743-59] (ab285162)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling Brevican with ab285162 at 1/2000 (0.295 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ , Polymer Refine Detection) was used. Positive staining on rat cerebrum. The section was incubated with ab285162 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ , Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Frozen sections) - Anti-Brevican antibody [EPR25743-59] (ab285162)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling Brevican with ab285162 at 1/50 (19.72 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 μg/ml) dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbedat 1/1000 (2 μg/ml) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brevican antibody [EPR25743-59] (ab285162)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Brevican with ab285162 at 1/2000 (0.295 ug/ml) followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used. Negative control: No staining on human kidney. The section was incubated with ab285162 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^®, Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Frozen sections) - Anti-Brevican antibody [EPR25743-59] (ab285162)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney (fresh) tissue labeling Brevican with ab285162 at 1/50 (19.72 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green). Negative control: No staining on rat kidney(PMID: 32559242) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-Brevican antibody [EPR25743-59] (ab285162)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney (fresh) tissue labeling Brevican with ab285162 at 1/50 (19.72 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green). Negative control: No staining on mouse kidney (PMID: 32559242) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.