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AB74221

Anti-BRF1 antibody

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(2 Publications)

Rabbit Polyclonal BRF1 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human BRF1 aa 550-600.

View Alternative Names

BRF, GTF3B, TAF3B2, TAF3C, BRF1, Transcription factor IIIB 90 kDa subunit, TFIIIB90, hTFIIIB90, B-related factor 1, BRF-1, hBRF

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRF1 antibody (AB74221)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRF1 antibody (AB74221)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BRF1 with ab74221 at 1/200 (1µg/ml). Detection : DAB.

Immunoprecipitation - Anti-BRF1 antibody (AB74221)
  • IP

Unknown

Immunoprecipitation - Anti-BRF1 antibody (AB74221)

HeLa whole cell lysate (1mg) was subjected to immunoprecipitation using ab74221 (3μg/mg lysate; lane 1) or a control IgG antibody (lane 2). 20% of the immunoprecipitates were used for Western blotting and bands were identified using ab74221 at a concentration of 1μg/ml. A band at the predicted molecular weight of 74kDa was detected using chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-BRF1 antibody (ab74221)

Predicted band size: 74 kDa

false

Western blot - Anti-BRF1 antibody (AB74221)
  • WB

Unknown

Western blot - Anti-BRF1 antibody (AB74221)

All lanes:

Western blot - Anti-BRF1 antibody (ab74221) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 74 kDa

Observed band size: 140 kDa,200 kDa,74 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, IP, WB

applications

Immunogen

Synthetic Peptide within Human BRF1 aa 550-600. The exact immunogen used to generate this antibody is proprietary information.

Q92994

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.
See full target information BRF1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:3007 PubMed35637192

2022

A cancer-associated RNA polymerase III identity drives robust transcription and expression of snaR-A noncoding RNA.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin Van Bortle,David P Marciano,Qing Liu,Tristan Chou,Andrew M Lipchik,Sanjay Gollapudi,Benjamin S Geller,Emma Monte,Rohinton T Kamakaka,Michael P Snyder

eLife 4:e06283 PubMed26067234

2015

MAF1 represses CDKN1A through a Pol III-dependent mechanism.

Applications

ChIP

Species

Human

Yu-Ling Lee,Yuan-Ching Li,Chia-Hsin Su,Chun-Hui Chiao,I-Hsuan Lin,Ming-Ta Hsu
View all publications
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Product promise

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