AB194442

Anti-BRF2 antibody - N-terminal

Name: Anti-BRF2 antibody - N-terminal (ab194442) | Abcam
Brand: Abcam Limited
SKU: AB194442

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(2 Publications)

Mouse Polyclonal BRF2 antibody. N-terminal. Suitable for WB and reacts with Recombinant fragment - Human, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BRF2 aa 1-100.

View Alternative Names

BRFU, PRO1470, BRF2, Transcription factor IIIB 50 kDa subunit, TFIIIB50, hTFIIIB50, B-related factor 2, hBRFU, BRF-2

2 Images

Supplier Data

Western blot - Anti-BRF2 antibody - N-terminal (AB194442)

All lanes:

Western blot - Anti-BRF2 antibody - N-terminal (ab194442) at 1/1000 dilution

All lanes:

Human ovarian cancer tissue lysate at 50 µg

Predicted band size: 47 kDa

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Supplier Data

Western blot - Anti-BRF2 antibody - N-terminal (AB194442)

The observed band size may not correspond to the predicted protein molecular weight as the immunogen (recombinant fragment) was used for the test lane.

All lanes:

Western blot - Anti-BRF2 antibody - N-terminal (ab194442) at 1/2500 dilution

All lanes:

BRF2 recombinant protein (immunizing peptide) at 0.2 µg

Predicted band size: 47 kDa

Observed band size: 37 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human BRF2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9HAW0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Constituents: 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites (PubMed : 11040218, PubMed : 11121026, PubMed : 11564744, PubMed : 26638071). Contributes to the regulation of gene expression; functions as activator in the absence of oxidative stress (PubMed : 26638071). Down-regulates expression of target genes in response to oxidative stress (PubMed : 26638071). Overexpression protects cells against apoptosis in response to oxidative stress (PubMed : 26638071).

See full target information BRF2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 22:1767-1774 PubMed32705258

2020

Silencing of BRF2 inhibits the growth and metastasis of lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Bian,Qiu Li,Qiaolian Li,Ruigen Pan

Cancer research and treatment 50:936-949 PubMed29025258

2017

MicroRNA-373 Inhibits Cell Proliferation and Invasion via Targeting BRF2 in Human Non-small Cell Lung Cancer A549 Cell Line.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wang,Junfeng Qu,Li Zhou,Fei Liao,Ju Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BRF2 antibody - N-terminal

Western blot - Anti-BRF2 antibody - N-terminal (AB194442)

Western blot - Anti-BRF2 antibody - N-terminal (AB194442)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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