Mouse Polyclonal BRF2 antibody. N-terminal. Suitable for WB and reacts with Recombinant fragment - Human, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BRF2 aa 1-100.
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Publications
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- Molecular medicine reports 22:1767-17742020Silencing of BRF2 inhibits the growth and metastasis of lung cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Bian et. al.PubMed 32705258
- Cancer research and treatment 50:936-9492017MicroRNA-373 Inhibits Cell Proliferation and Invasion via Targeting BRF2 in Human Non-small Cell Lung Cancer A549 Cell Line.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Wang,Junfeng Qu,Li Zhou,Fei Liao,Ju WangPubMed 29025258
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine)
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human BRF2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HAW0
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Cow | Predicted |
| Cynomolgus monkey | Predicted |
| Recombinant fragment - Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info 1/500 - 1/2500 | Notes - |
Species Human | Dilution info 1/500 - 1/2500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cynomolgus monkey | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites (PubMed:11040218, PubMed:11121026, PubMed:11564744, PubMed:26638071). Contributes to the regulation of gene expression; functions as activator in the absence of oxidative stress (PubMed:26638071). Down-regulates expression of target genes in response to oxidative stress (PubMed:26638071). Overexpression protects cells against apoptosis in response to oxidative stress (PubMed:26638071).
Alternative names
BRFU, PRO1470, BRF2, Transcription factor IIIB 50 kDa subunit, TFIIIB50, hTFIIIB50, B-related factor 2, hBRFU, BRF-2
Recommended products
Mouse Polyclonal BRF2 antibody. N-terminal. Suitable for WB and reacts with Recombinant fragment - Human, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BRF2 aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine)
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human BRF2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HAW0
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-BRF2 antibody - N-terminal (ab194442)
The observed band size may not correspond to the predicted protein molecular weight as the immunogen (recombinant fragment) was used for the test lane.
All lanes: Western blot - Anti-BRF2 antibody - N-terminal (ab194442) at 1/2500 dilution
All lanes: BRF2 recombinant protein (immunizing peptide) at 0.2 µg
Predicted band size: 47 kDa
Observed band size: 37 kDa
Western blot - Anti-BRF2 antibody - N-terminal (ab194442)
All lanes: Western blot - Anti-BRF2 antibody - N-terminal (ab194442) at 1/1000 dilution
All lanes: Human ovarian cancer tissue lysate at 50 µg
Predicted band size: 47 kDa
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