Anti-BRG1 antibody

• WB

Unknown

Western blot - Anti-BRG1 antibody (AB4081)

Rabbit polyclonal to BRG1 (ab4081) at 1/500 on HeLa Nuclear extract (20 ug per lane).

Lane 1 : ab4081 (1/500)
Lane 2 : ab4081 (1/500) + blocking peptide (1 ug)

Secondary antibody : Goat anti-rabbit HRP - ab6721

All lanes:

Western blot - Anti-BRG1 antibody (ab4081) at 1/500 dilution

Lane 1:

HeLa Nuclear extract at 20 µg

Lane 2:

HeLa Nuclear extract at 20 µg with Human BRG1 peptide (<a href='/en-us/products/unavailable/human-brg1-peptide-ab13736'>ab13736</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>)

Predicted band size: 185 kDa

Observed band size: 235 kDa

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• WB

Project

Western blot - Anti-BRG1 antibody (AB4081)

All lanes:

Western blot - Anti-BRG1 antibody (ab4081) at 1 µg/mL

Lane 1:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 2:

Jurkat nuclear extract lysate at 10 µg

Lane 3:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

Western blot - Hep G2 nuclear extract lysate (<a href='/en-us/products/tissue-lysates/hep-g2-nuclear-extract-lysate-ab14660'>ab14660</a>) at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 185 kDa

Observed band size: 235 kDa,40 kDa

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• WB

AbReview26826****

Western blot - Anti-BRG1 antibody (AB4081)

Whole cell lysate prepared from HeLa cells was loaded at 150000 cells.
ab4081 used at a 1/1000 dilution.
The secondary used was an HRP conjugated donkey polyclonal used at a 1/5000 dilution.

All lanes:

Western blot - Anti-BRG1 antibody (ab4081)

Predicted band size: 185 kDa

Observed band size: 225 kDa

true

Exposure time: 5min

This image is courtesy of an anonymous abreview.

• WB

Lab

Western blot - Anti-BRG1 antibody (AB4081)

Lanes 1 - 4 : Merged signal (red and green).

Green - ab4081 observed at 240 kDa.

Red - loading control, Mouse monoclonal Anti-Vinculin antibody, observed at 124 kDa.

ab4081 was shown to recognize BRG1 when BRG1 knockout samples were used, along with additional cross-reactive bands.

Wild-type and BRG1 knockout samples were subjected to SDS-PAGE. ab4081 and loading control to Vinculin were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773 - Green) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776 - Red) secondary antibodies at 1 : 10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-BRG1 antibody (ab4081) at 1/500 dilution

Lane 1:

Wild-type HAP1 cell lysate at 40 µg

Lane 2:

BRG1 knockout HAP1 cell lysate at 40 µg

Lane 3:

HeLa (Human epithelial adenocarcinoma cell line) whole cell lysate at 40 µg

Lane 4:

K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 185 kDa

false

• WB

Unknown

Western blot - Anti-BRG1 antibody (AB4081)

All lanes:

Western blot - Anti-BRG1 antibody (ab4081) at 1/1000 dilution

All lanes:

Western blot - Recombinant human BRG1 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-brg1-protein-ab82237'>ab82237</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 185 kDa

true

Exposure time: 8min