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Mouse Recombinant Monoclonal BRG1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

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Images

Western blot - Anti-BRG1 antibody [G-SMARCA4-2] - Mouse IgG2a (Chimeric) (AB282705), expandable thumbnail
  • Western blot - Anti-BRG1 antibody [G-SMARCA4-2] - Mouse IgG2a (Chimeric) (AB282705), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-BRG1 antibody [G-SMARCA4-2] - Mouse IgG2a (Chimeric) (AB282705), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-BRG1 antibody [G-SMARCA4-2] - Mouse IgG2a (Chimeric) (AB282705), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)
Human
Not recommended
Tested
Not recommended
Tested
Mouse
Not recommended
Tested
Not recommended
Tested
Rat
Not recommended
Tested
Not recommended
Expected

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

-

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating the calcium-dependent release of a repressor complex and the recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by SMARCA4-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves the release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development, a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues (By similarity). Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1. Binds via DLX1 to enhancers located in the intergenic region between DLX5 and DLX6 and this binding is stabilized by the long non-coding RNA (lncRNA) Evf2 (By similarity). Binds to RNA in a promiscuous manner (By similarity). Binding to RNAs including lncRNA Evf2 leads to inhibition of SMARCA4 ATPase and chromatin remodeling activities (By similarity). In brown adipose tissue, involved in the regulation of thermogenic genes expression (By similarity).

Alternative names

BAF190A, BRG1, SNF2B, SNF2L4, SMARCA4, Transcription activator BRG1, ATP-dependent helicase SMARCA4, BRG1-associated factor 190A, Mitotic growth and transcription activator, Protein BRG-1, Protein brahma homolog 1, SNF2-beta, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4

Recommended products

Mouse Recombinant Monoclonal BRG1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
G-SMARCA4-2
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

ab282705 is a mouse monoclonal chimeric antibody.

What are the advantages of a recombinant monoclonal antibody?

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Collaborations
This antibody was developed by the Structural Genomics Consortium and manufactured by Abcam.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-BRG1 antibody [G-SMARCA4-2] - Mouse IgG2a (Chimeric) (ab282705), expandable thumbnail

    Western blot - Anti-BRG1 antibody [G-SMARCA4-2] - Mouse IgG2a (Chimeric) (ab282705)

    Blocking and diluting buffer and concentration: 3% NFDM/TBST

    Lanes 1-3: Merged signal (red and green). Green - ab282705 observed at 185 kDa. Red - loading control Anti-Vinculin antibody [EPR8185] - Loading Control ab129002 observed at 124 kDa.

    Lanes 1-2: ab282705 Anti-BRG1 antibody was shown to react with BRG1 in HAP1 cells in Western blot. Loss of signal was observed when BRG1 knockout sample was used. Wild-type and BRG1 knockout samples were subjected to SDS-PAGE.

    ab282705 and Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) were incubated at 4°C overnight at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680CW) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-BRG1 antibody [G-SMARCA4-2] - Mouse IgG2a (Chimeric) (ab282705) at 1/1000 dilution

    Lane 1: Wild type HAP1(human chronic myelogenous leukemia near-haploid cell line), whole cell lysate at 20 µg

    Lane 2: BRG1 knockout HAP1(human chronic myelogenous leukemia near-haploid cell line), whole cell lysate at 20 µg

    Lane 3: K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Mouse IgG H&L (IRDye® 800CW) (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/10000 dilution

    Predicted band size: 185 kDa

    Observed band size: 185 kDa

  • Western blot - Anti-BRG1 antibody [G-SMARCA4-2] - Mouse IgG2a (Chimeric) (ab282705), expandable thumbnail

    Western blot - Anti-BRG1 antibody [G-SMARCA4-2] - Mouse IgG2a (Chimeric) (ab282705)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-BRG1 antibody [G-SMARCA4-2] - Mouse IgG2a (Chimeric) (ab282705) at 1/1000 dilution

    Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

    Lane 2: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 185 kDa

    Observed band size: 185 kDa

  • Flow Cytometry (Intracellular) - Anti-BRG1 antibody [G-SMARCA4-2] - Mouse IgG2a (Chimeric) (ab282705), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-BRG1 antibody [G-SMARCA4-2] - Mouse IgG2a (Chimeric) (ab282705)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized K-562 (human chronic myelogenous leukemia lymphoblast) cells labelling BRG1 with ab282705 at 1/100 dilution (1 μg) (Red) compared with a mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-BRG1 antibody [G-SMARCA4-2] - Mouse IgG2a (Chimeric) (ab282705), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-BRG1 antibody [G-SMARCA4-2] - Mouse IgG2a (Chimeric) (ab282705)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling BRG1 with ab282705 at 1/100 dilution (1 μg) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution was used as the secondary antibody.

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Product protocols

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