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AB101389

Anti-BRI3 antibody

4

(2 Reviews)

|

(2 Publications)

Rabbit Polyclonal BRI3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ITM2C aa 100-300.

View Alternative Names

BRI3, hucep-14, NPD018, PSEC0047, ITM2C, Integral membrane protein 2C, Cerebral protein 14, Transmembrane protein BRI3

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRI3 antibody (AB101389)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRI3 antibody (AB101389)

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using ab101389 at 1/100 dilution.

Western blot - Anti-BRI3 antibody (AB101389)
  • WB

Unknown

Western blot - Anti-BRI3 antibody (AB101389)

12% SDS PAGE

All lanes:

Western blot - Anti-BRI3 antibody (ab101389) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 30 kDa,36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ITM2C aa 100-300. The exact immunogen used to generate this antibody is proprietary information.

Q9NQX7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Use 10mM Citrate buffer or Tris-EDTA buffer (PH8.0)</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Negative regulator of amyloid-beta peptide production. May inhibit the processing of APP by blocking its access to alpha- and beta-secretase. Binding to the beta-secretase-cleaved APP C-terminal fragment is negligible, suggesting that ITM2C is a poor gamma-secretase cleavage inhibitor. May play a role in TNF-induced cell death and neuronal differentiation (By similarity).
See full target information ITM2C
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 30:3478-3491.e6 PubMed32160551

2020

NRBP1-Containing CRL2/CRL4A Regulates Amyloid β Production by Targeting BRI2 and BRI3 for Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi Yasukawa,Aya Tsutsui,Chieri Tomomori-Sato,Shigeo Sato,Anita Saraf,Michael P Washburn,Laurence Florens,Tohru Terada,Kentaro Shimizu,Ronald C Conaway,Joan W Conaway,Teijiro Aso

Journal of Alzheimer's disease reports 2:27-39 PubMed30480246

2018

The Bri2 and Bri3 BRICHOS Domains Interact Differently with Aβ and Alzheimer Amyloid Plaques.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa Dolfe,Simone Tambaro,Helene Tigro,Marta Del Campo,Jeroen J M Hoozemans,Birgitta Wiehager,Caroline Graff,Bengt Winblad,Maria Ankarcrona,Margit Kaldmäe,Charlotte E Teunissen,Annica Rönnbäck,Jan Johansson,Jenny Presto
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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