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AB126517

Anti-BRIP1 antibody

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(2 Publications)

Rabbit Polyclonal BRIP1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MRPL36 aa 1 to C-terminus.

View Alternative Names

BRIP1, MRPL36, Large ribosomal subunit protein bL36m, BRCA1-interacting protein 1, L36mt, MRP-L36

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRIP1 antibody (AB126517)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRIP1 antibody (AB126517)

Immunohistochemical analysis of human colon tissue labeling BRIP1 in the cytoplasm of glandular cells with ab126517 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-BRIP1 antibody (AB126517)
  • WB

Supplier Data

Western blot - Anti-BRIP1 antibody (AB126517)

All lanes:

Western blot - Anti-BRIP1 antibody (ab126517) at 0.4 µg/mL

Lane 1:

RT4 (Human urinary bladder cancer cell line) whole cell lysate

Lane 2:

U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate

Predicted band size: 12 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-BRIP1 antibody (AB126517)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BRIP1 antibody (AB126517)

Immunohistochemical analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cells labeling BRIP1 in the nuclear bodies and mitochondria with ab126517 at 2 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human MRPL36 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9P0J6

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 80:361 PubMed37971521

2023

GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Wang,Taru Hilander,Xiaonan Liu,Hoi Ying Tsang,Ove Eriksson,Christopher B Jackson,Markku Varjosalo,Hongxia Zhao

Frontiers in immunology 14:1179742 PubMed37622116

2023

Identification of cuproptosis and immune-related gene prognostic signature in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wentao Zhang,Haizeng Qu,Xiaoqing Ma,Liang Li,Yanjun Wei,Ye Wang,Renya Zeng,Yuanliu Nie,Chenggui Zhang,Ke Yin,Fengge Zhou,Zhe Yang
View all publications

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