Rabbit Recombinant Monoclonal Brk/PTK6 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
IP | WB | Flow Cyt (Intra) | IHC-P | |
---|---|---|---|---|
Human | Tested | Expected | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Non-receptor tyrosine-protein kinase implicated in the regulation of a variety of signaling pathways that control the differentiation and maintenance of normal epithelia, as well as tumor growth. Function seems to be context dependent and differ depending on cell type, as well as its intracellular localization. A number of potential nuclear and cytoplasmic substrates have been identified. These include the RNA-binding proteins: KHDRBS1/SAM68, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF; transcription factors: STAT3 and STAT5A/B and a variety of signaling molecules: ARHGAP35/p190RhoGAP, PXN/paxillin, BTK/ATK, STAP2/BKS. Associates also with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, or for which PTK6 may play an adapter-like role. These proteins include ADAM15, EGFR, ERBB2, ERBB3 and IRS4. In normal or non-tumorigenic tissues, PTK6 promotes cellular differentiation and apoptosis. In tumors PTK6 contributes to cancer progression by sensitizing cells to mitogenic signals and enhancing proliferation, anchorage-independent survival and migration/invasion. Association with EGFR, ERBB2, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase. Contributes to migration and proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/p190RhoGAP, which promotes association with RASA1/p120RasGAP, inactivating RhoA while activating RAS. EGF stimulation resulted in phosphorylation of PNX/Paxillin by PTK6 and activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. PTK6 activates STAT3 and STAT5B to promote proliferation. Nuclear PTK6 may be important for regulating growth in normal epithelia, while cytoplasmic PTK6 might activate oncogenic signaling pathways.Isoform 2 inhibits PTK6 phosphorylation and PTK6 association with other tyrosine-phosphorylated proteins.
BRK, PTK6, Protein-tyrosine kinase 6, Breast tumor kinase, Tyrosine-protein kinase BRK
Rabbit Recombinant Monoclonal Brk/PTK6 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR21051-96
Affinity purification Protein A
Blue Ice
+4°C
+4°C
Do Not Freeze
ab234102 is the carrier-free version of Anti-Brk/PTK6 antibody [EPR21051-96] ab233392.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
Brk known also as PTK6 is a non-receptor tyrosine kinase. Its molecular mass is approximately 52 kDa. PTK6 is expressed in various tissues with higher levels found in epithelial tissues such as breast skin and gastrointestinal tissues. Mechanically PTK6 phosphorylates different substrates affecting their function and participating in signal transduction. It acts intracellularly influencing cellular processes like proliferation differentiation and migration.
PTK6 plays a role in cellular signaling systems influencing growth and survival. It does not belong to a large protein complex but often interacts with several downstream proteins to exert its effects. PTK6 modulates pathways in normal epithelial tissue development and homeostasis. In addition it regulates functions like apoptosis and motility significant for tissue maintenance and repair.
PTK6 relates to key signaling cascades such as the MAPK and PI3K-Akt pathways. Within these pathways PTK6 is often associated with proteins like ERK1/2 and Akt which are important for cell survival and growth regulation. PTK6 can activate or inhibit these pathways influencing the cellular response to various stimuli and sometimes altering processes like cell cycle progression.
PTK6 has been implicated in the development and progression of certain cancers such as breast and prostate cancer. Elevated PTK6 expression levels have been linked to poor prognosis in these cancers where it appears to enhance tumor cell proliferation and invasion. The kinase interacts with proteins like HER2 and EGFR which are also involved in these cancers potentially affecting therapeutic responses and resistance to treatments.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Brk/PTK6 with Anti-Brk/PTK6 antibody [EPR21051-96] ab233392 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and nuclear staining in surface epithelium of human colon (PMID: 10430081) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Brk/PTK6 antibody [EPR21051-96] ab233392).
Brk/PTK6 was immunoprecipitated from 0.35 mg of T-47D (human ductal breast epithelial tumor cell line) whole cell lysate with Anti-Brk/PTK6 antibody [EPR21051-96] ab233392 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-Brk/PTK6 antibody [EPR21051-96] ab233392 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.
Lane 1: T-47D whole cell lysate 10 μg (Input).
Lane 2: Anti-Brk/PTK6 antibody [EPR21051-96] ab233392 IP in T-47D whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Brk/PTK6 antibody [EPR21051-96] ab233392 in T-47D whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
IgG heavy chain: PMID: 18781181
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Brk/PTK6 antibody [EPR21051-96] ab233392).
All lanes: Immunoprecipitation - Anti-Brk/PTK6 antibody [EPR21051-96] (Anti-Brk/PTK6 antibody [EPR21051-96] ab233392)
Predicted band size: 52 kDa
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized T-47D (human ductal breast epithelial tumor cell line) cell line labeling Brk/PTK6 with Anti-Brk/PTK6 antibody [EPR21051-96] ab233392 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Brk/PTK6 antibody [EPR21051-96] ab233392).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Brk/PTK6 with Anti-Brk/PTK6 antibody [EPR21051-96] ab233392 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and nuclear staining in and membranous staining on human breast cancer cells is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Brk/PTK6 antibody [EPR21051-96] ab233392).
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