Goat Polyclonal Brn-2 antibody. Suitable for WB and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human POU3F2 aa 1-100.
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- eLife 10:2021Olig3 regulates early cerebellar development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElijah D Lowenstein,Aleksandra Rusanova,Jonas Stelzer,Marc Hernaiz-Llorens,Adrian E Schroer,Ekaterina Epifanova,Francesca Bladt,Eser Göksu Isik,Sven Buchert,Shiqi Jia,Victor Tarabykin,Luis R Hernandez-MirandaPubMed 33591268
- Pathology international 63:158-682013POU domain transcription factor BRN2 is crucial for expression of ASCL1, ND1 and neuroendocrine marker molecules and cell growth in small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Ishii,Hanako Sato,Masashi Sakaeda,Yukiko Shishido-Hara,Chie Hiramatsu,Hiroshi Kamma,Hiroaki Shimoyamada,Masachika Fujiwara,Tetsuya Endo,Ichiro Aoki,Takuya YazawaPubMed 23530560
- Laboratory investigation; a journal of technical m 93:408-212013Neural lineage-specific homeoprotein BRN2 is directly involved in TTF1 expression in small-cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasashi Sakaeda et. al.PubMed 23358112
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human POU3F2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P20265
Reactivity data
Select an application| WB | |
|---|---|
| Mouse | Tested |
| Cow | Predicted |
| Pig | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.10000-0.30000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig | Dilution info - | Notes - |
Associated Products
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4 products for Alternative Product
Target data
Function
Transcription factor that plays a key role in neuronal differentiation (By similarity). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity). Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (By similarity). Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (By similarity).
Alternative names
BRN2, OCT7, OTF7, POU3F2, Brain-specific homeobox/POU domain protein 2, Nervous system-specific octamer-binding transcription factor N-Oct-3, Octamer-binding protein 7, Octamer-binding transcription factor 7, Brain-2, Brn-2, Oct-7, OTF-7
Recommended products
Goat Polyclonal Brn-2 antibody. Suitable for WB and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human POU3F2 aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human POU3F2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P20265
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab101726 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Brn-2 also known as POU domain class 3 transcription factor 2 (POU3F2) is a transcription factor with a molecular weight of approximately 48 kDa. This protein plays a significant role in the development and function of the central nervous system. It is abundantly expressed in neuronal tissues and specific types of melanocytes. Brn-2 belongs to the POU domain family of proteins which are pivotal in regulating gene expression during development.
- Biological function summary
Brn-2 regulates the expression of genes critical for neural differentiation and development. It acts as a transcriptional activator that binds to specific DNA sequences. Brn-2 does not act alone; it often participates in regulatory complexes with other transcription factors. Through these interactions Brn-2 influences the fate of neural progenitor cells and is involved in the regulation of neural stem cell maintenance.
- Pathways
Brn-2 plays a role in the Wnt signaling pathway and the Notch signaling pathway. Both of these pathways are integral to cell fate determination during development. In the Wnt pathway Brn-2 may interact with proteins such as β-catenin which modulates the expression of genes involved in cell proliferation and differentiation. Additionally Brn-2's role in the Notch pathway can influence the activity of proteins like RBPJ impacting cell communication and developmental processes.
- Associated diseases and disorders
Brn-2 has been linked to melanoma and gliomas. In melanoma Brn-2 can interact with other proteins such as MITF influencing melanocyte development and contributing to cancer progression. In glioma Brn-2's aberrant expression might affect cellular pathways that lead to uncontrolled proliferation and tumor formation. Understanding these connections provides insights into potential therapeutic targets for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Brn-2 antibody (ab101726)
Primary incubation was 1 hour.
All lanes: Western blot - Anti-Brn-2 antibody (ab101726) at 0.1 µg/mL
All lanes: Mouse brain lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 46 kDa
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