AB101726

Anti-Brn-2 antibody

Name: Anti-Brn-2 antibody (ab101726) | Abcam
Brand: Abcam Limited
SKU: AB101726
Price: 535 USD
Availability: InStock

Be the first to review this product! Submit a review

(4 Publications)

Goat Polyclonal Brn-2 antibody. Suitable for WB and reacts with Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human POU3F2 aa 1-100.

View Alternative Names

BRN2, OCT7, OTF7, POU3F2, Brain-specific homeobox/POU domain protein 2, Nervous system-specific octamer-binding transcription factor N-Oct-3, Octamer-binding protein 7, Octamer-binding transcription factor 7, Brain-2, Brn-2, Oct-7, OTF-7

1 Images

Unknown

Western blot - Anti-Brn-2 antibody (AB101726)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-Brn-2 antibody (ab101726) at 0.1 µg/mL

All lanes:

Mouse brain lysate in RIPA buffer at 35 µg

Predicted band size: 46 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Synthetic Peptide within Human POU3F2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P20265

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab101726 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Brn-2 also known as POU domain class 3 transcription factor 2 (POU3F2) is a transcription factor with a molecular weight of approximately 48 kDa. This protein plays a significant role in the development and function of the central nervous system. It is abundantly expressed in neuronal tissues and specific types of melanocytes. Brn-2 belongs to the POU domain family of proteins which are pivotal in regulating gene expression during development.

Biological function summary

Brn-2 regulates the expression of genes critical for neural differentiation and development. It acts as a transcriptional activator that binds to specific DNA sequences. Brn-2 does not act alone; it often participates in regulatory complexes with other transcription factors. Through these interactions Brn-2 influences the fate of neural progenitor cells and is involved in the regulation of neural stem cell maintenance.

Pathways

Brn-2 plays a role in the Wnt signaling pathway and the Notch signaling pathway. Both of these pathways are integral to cell fate determination during development. In the Wnt pathway Brn-2 may interact with proteins such as β-catenin which modulates the expression of genes involved in cell proliferation and differentiation. Additionally Brn-2's role in the Notch pathway can influence the activity of proteins like RBPJ impacting cell communication and developmental processes.

Brn-2 has been linked to melanoma and gliomas. In melanoma Brn-2 can interact with other proteins such as MITF influencing melanocyte development and contributing to cancer progression. In glioma Brn-2's aberrant expression might affect cellular pathways that lead to uncontrolled proliferation and tumor formation. Understanding these connections provides insights into potential therapeutic targets for these conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transcription factor that plays a key role in neuronal differentiation (By similarity). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity). Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (By similarity). Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (By similarity).

See full target information POU3F2

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Development (Cambridge, England) 152: PubMed40207459

2025

Differential contribution of P73+ Cajal-Retzius cells and Reelin to cortical morphogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Vicente Elorriaga,Benoît Bouloudi,Elodie Delberghe,Yoann Saillour,Juliette S Morel,Patrick Azzam,Matthieu X Moreau,Rolf Stottmann,Nadia Bahi-Buisson,Alessandra Pierani,Nathalie Spassky,Frédéric Causeret

eLife 10: PubMed33591268

2021

Olig3 regulates early cerebellar development.

Applications

Unspecified application

Species

Unspecified reactive species

Elijah D Lowenstein,Aleksandra Rusanova,Jonas Stelzer,Marc Hernaiz-Llorens,Adrian E Schroer,Ekaterina Epifanova,Francesca Bladt,Eser Göksu Isik,Sven Buchert,Shiqi Jia,Victor Tarabykin,Luis R Hernandez-Miranda

Pathology international 63:158-68 PubMed23530560

2013

POU domain transcription factor BRN2 is crucial for expression of ASCL1, ND1 and neuroendocrine marker molecules and cell growth in small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Ishii,Hanako Sato,Masashi Sakaeda,Yukiko Shishido-Hara,Chie Hiramatsu,Hiroshi Kamma,Hiroaki Shimoyamada,Masachika Fujiwara,Tetsuya Endo,Ichiro Aoki,Takuya Yazawa

Laboratory investigation; a journal of technical m 93:408-21 PubMed23358112

2013

Neural lineage-specific homeoprotein BRN2 is directly involved in TTF1 expression in small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Masashi Sakaeda,Hanako Sato,Jun Ishii,Chie Miyata,Hiroshi Kamma,Yukiko Shishido-Hara,Hiroaki Shimoyamada,Masachika Fujiwara,Tetsuya Endo,Ryota Tanaka,Haruhiko Kondo,Tomoyuki Goya,Ichiro Aoki,Takuya Yazawa

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Brn-2 antibody

Western blot - Anti-Brn-2 antibody (AB101726)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise