AB137469

Anti-Brn-2 antibody

Name: Anti-Brn-2 antibody (ab137469) | Abcam
Brand: Abcam Limited
SKU: AB137469
Price: 525 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(10 Publications)

Rabbit Polyclonal Brn-2 antibody. Suitable for IHC-P, WB, ChIP, ICC/IF, IHC-Fr, IP and reacts with Human, Rat samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human POU3F2 aa 200 to C-terminus.

View Alternative Names

BRN2, OCT7, OTF7, POU3F2, Brain-specific homeobox/POU domain protein 2, Nervous system-specific octamer-binding transcription factor N-Oct-3, Octamer-binding protein 7, Octamer-binding transcription factor 7, Brain-2, Brn-2, Oct-7, OTF-7

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brn-2 antibody (AB137469)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brn-2 antibody (AB137469)

Immunohistochemical analysis of paraffin embedded TOV-21G xenograft labelling Brn-2 with ab137469 antibody at a dilution of 1/500.

Immunocytochemistry/ Immunofluorescence - Anti-Brn-2 antibody (AB137469)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Brn-2 antibody (AB137469)

Immunofluorescence analysis of SK-N-AS cells fixed in 4% paraformaldehyde at RT for 15 min labelling Brn2 protein at nucleus using ab137469 at a 1/1000 dilution (Green). beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by a beta Tubulin 3/ Tuj1 antibody at 1/500 dilution (Red).

Supplier Data

Immunoprecipitation - Anti-Brn-2 antibody (AB137469)

Samples were separated by 10% SDS-PAGE.
The immunoprecipitated Brn2 protein was detected by Brn2 antibody at a 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-Brn-2 antibody (ab137469) at 1/1000 dilution

Lane 1:

293T whole cell lysate/extract (Input) at 50 µg

Lane 2:

293T whole cell lysate/extract with rabbit IgG C (-)

Lane 3:

293T whole cell lysate/extract (+)

Secondary

All lanes:

Anti-Rabbit IgG Antibody

Observed band size: 55 kDa

false

ChIP

Supplier Data

ChIP - Anti-Brn-2 antibody (AB137469)

Brn2 antibody immunoprecipitates Brn2 protein-DNA in ChIP experiments. ChIP Sample : 293T whole cell lysate/extract A. 5 µg preimmune rabbit IgG B. 5 µg of Brn2 antibody The precipitated DNA was detected by PCR with primer set targeting to MITF promoter.

Immunohistochemistry (Frozen sections) - Anti-Brn-2 antibody (AB137469)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Brn-2 antibody (AB137469)

Immunohistochemical analysis of Frozen sectioned E13.5 Rat brain staining Brn2 protein expression at nucleus with ab137469 at a dilution of 1/250 (Green). beta Tubulin 3/TUJ1, a mature neuron marker, stained by anti beta Tubulin 3/TUJ1 antibody diluted at 1/500 dilution (Red). The nuclear staining is DAPI (Blue).

Supplier Data

Western blot - Anti-Brn-2 antibody (AB137469)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Brn-2 antibody (ab137469) at 1/1000 dilution

All lanes:

A375 Whole cell extract at 30 µg

Observed band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IP, WB, IHC-Fr, ICC/IF, IHC-P, ChIP

applications

Immunogen

Recombinant Fragment Protein within Human POU3F2 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P20265

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Brn-2 also known as POU domain class 3 transcription factor 2 (POU3F2) is a transcription factor with a molecular weight of approximately 48 kDa. This protein plays a significant role in the development and function of the central nervous system. It is abundantly expressed in neuronal tissues and specific types of melanocytes. Brn-2 belongs to the POU domain family of proteins which are pivotal in regulating gene expression during development.

Biological function summary

Brn-2 regulates the expression of genes critical for neural differentiation and development. It acts as a transcriptional activator that binds to specific DNA sequences. Brn-2 does not act alone; it often participates in regulatory complexes with other transcription factors. Through these interactions Brn-2 influences the fate of neural progenitor cells and is involved in the regulation of neural stem cell maintenance.

Pathways

Brn-2 plays a role in the Wnt signaling pathway and the Notch signaling pathway. Both of these pathways are integral to cell fate determination during development. In the Wnt pathway Brn-2 may interact with proteins such as β-catenin which modulates the expression of genes involved in cell proliferation and differentiation. Additionally Brn-2's role in the Notch pathway can influence the activity of proteins like RBPJ impacting cell communication and developmental processes.

Brn-2 has been linked to melanoma and gliomas. In melanoma Brn-2 can interact with other proteins such as MITF influencing melanocyte development and contributing to cancer progression. In glioma Brn-2's aberrant expression might affect cellular pathways that lead to uncontrolled proliferation and tumor formation. Understanding these connections provides insights into potential therapeutic targets for these conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transcription factor that plays a key role in neuronal differentiation (By similarity). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity). Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (By similarity). Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (By similarity).

See full target information POU3F2

style

left-column

Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6347 PubMed40634286

2025

Early developmental origins of cortical disorders modeled in human neural stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xoel Mato-Blanco,Suel-Kee Kim,Alexandre Jourdon,Shaojie Ma,Sang-Hun Choi,Alice M Giani,Miguel I Paredes,Andrew T N Tebbenkamp,Fuchen Liu,Alvaro Duque,Flora M Vaccarino,Nenad Sestan,Carlo Colantuoni,Pasko Rakic,Gabriel Santpere,Nicola Micali

Nature communications 16:5132 PubMed40461535

2025

Forebrain neural progenitors effectively integrate into host brain circuits and improve neural function after ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao He,Jiadong Chen,Yan Zhong,Peili Cen,Li Shen,Fei Huang,Jing Wang,Chentao Jin,Rui Zhou,Xiaohui Zhang,Anxin Wang,Jing Fan,Shuang Wu,Mengjiao Tu,Xiyi Qin,Xiaoyun Luo,Yu Zhou,Jieqiao Peng,Youyou Zhou,A Cahid Civelek,Mei Tian,Hong Zhang

Cell stem cell 30:312-332.e13 PubMed36796362

2023

Natural variation in gene expression and viral susceptibility revealed by neural progenitor cell villages.

Applications

Unspecified application

Species

Unspecified reactive species

Michael F Wells,James Nemesh,Sulagna Ghosh,Jana M Mitchell,Max R Salick,Curtis J Mello,Daniel Meyer,Olli Pietilainen,Federica Piccioni,Ellen J Guss,Kavya Raghunathan,Matthew Tegtmeyer,Derek Hawes,Anna Neumann,Kathleen A Worringer,Daniel Ho,Sravya Kommineni,Karrie Chan,Brant K Peterson,Joseph J Raymond,John T Gold,Marco T Siekmann,Emanuela Zuccaro,Ralda Nehme,Ajamete Kaykas,Kevin Eggan,Steven A McCarroll

The Journal of neuroscience : the official journal of the Society for Neuroscience 41:4335-4348 PubMed33893219

2021

Continuous Monitoring of Tau-Induced Neurotoxicity in Patient-Derived iPSC-Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Derek H Oakley,Naomi Klickstein,Caitlin Commins,Mirra Chung,Simon Dujardin,Rachel E Bennett,Bradley T Hyman,Matthew P Frosch

Cancer management and research 13:743-755 PubMed33531841

2021

Circ_0031242 Silencing Mitigates the Progression and Drug Resistance in DDP-Resistant Hepatoma Cells by the miR-924/POU3F2 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Fan,Lei Chen,Xiaojuan Wu,Tong Zhang

Redox biology 29:101402 PubMed31926626

2020

miR-30-5p-mediated ferroptosis of trophoblasts is implicated in the pathogenesis of preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Zhang,Yue He,Jian-Xia Wang,Ming-Hua Chen,Jian-Juan Xu,Min-Hui Jiang,Ya-Ling Feng,Yan-Fang Gu

Nature biomedical engineering 4:69-83 PubMed31844155

2019

Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaogang Yang,Junfeng Shi,Jing Xie,Yifan Wang,Jingyao Sun,Tongzheng Liu,Yarong Zhao,Xiuting Zhao,Xinmei Wang,Yifan Ma,Veysi Malkoc,Chiling Chiang,Weiye Deng,Yuanxin Chen,Yuan Fu,Kwang J Kwak,Yamin Fan,Chen Kang,Changcheng Yin,June Rhee,Paul Bertani,Jose Otero,Wu Lu,Kyuson Yun,Andrew S Lee,Wen Jiang,Lesheng Teng,Betty Y S Kim,L James Lee

Cell systems 8:122-135.e7 PubMed30772379

2019

Genome-Scale Transcriptional Regulatory Network Models of Psychiatric and Neurodegenerative Disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Jocelynn R Pearl,Carlo Colantuoni,Dani E Bergey,Cory C Funk,Paul Shannon,Bijoya Basu,Alex M Casella,Rediet T Oshone,Leroy Hood,Nathan D Price,Seth A Ament

Translational psychiatry 8:245 PubMed30410030

2018

Convergence of independent DISC1 mutations on impaired neurite growth via decreased UNC5D expression.

Applications

Unspecified application

Species

Unspecified reactive species

Priya Srikanth,Valentina N Lagomarsino,Richard V Pearse,Meichen Liao,Sulagna Ghosh,Ralda Nehme,Nicholas Seyfried,Kevin Eggan,Tracy L Young-Pearse

Translational psychiatry 8:77 PubMed29643329

2018

Shared effects of DISC1 disruption and elevated WNT signaling in human cerebral organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Priya Srikanth,Valentina N Lagomarsino,Christina R Muratore,Steven C Ryu,Amy He,Walter M Taylor,Constance Zhou,Marlise Arellano,Tracy L Young-Pearse

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Brn-2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brn-2 antibody (AB137469)

Immunocytochemistry/ Immunofluorescence - Anti-Brn-2 antibody (AB137469)

Immunoprecipitation - Anti-Brn-2 antibody (AB137469)

ChIP - Anti-Brn-2 antibody (AB137469)

Immunohistochemistry (Frozen sections) - Anti-Brn-2 antibody (AB137469)

Western blot - Anti-Brn-2 antibody (AB137469)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise