Rabbit Polyclonal Brn-2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human POU3F2 aa 200 to C-terminus.
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Publications
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- The Journal of neuroscience : the official journal of the Society for Neuroscience 41:4335-43482021Continuous Monitoring of Tau-Induced Neurotoxicity in Patient-Derived iPSC-Neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDerek H Oakley,Naomi Klickstein,Caitlin Commins,Mirra Chung,Simon Dujardin,Rachel E Bennett,Bradley T Hyman,Matthew P FroschPubMed 33893219
- Cancer management and research 13:743-7552021Circ_0031242 Silencing Mitigates the Progression and Drug Resistance in DDP-Resistant Hepatoma Cells by the miR-924/POU3F2 Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Fan et. al.PubMed 33531841
- Nature biomedical engineering 4:69-832019Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhaogang Yang,Junfeng Shi,Jing Xie,Yifan Wang,Jingyao Sun,Tongzheng Liu,Yarong Zhao,Xiuting Zhao,Xinmei Wang,Yifan Ma,Veysi Malkoc,Chiling Chiang,Weiye Deng,Yuanxin Chen,Yuan Fu,Kwang J Kwak,Yamin Fan,Chen Kang,Changcheng Yin,June Rhee,Paul Bertani,Jose Otero,Wu Lu,Kyuson Yun,Andrew S Lee,Wen Jiang,Lesheng Teng,Betty Y S Kim,L James LeePubMed 31844155
- Cell systems 8:122-135.e72019Genome-Scale Transcriptional Regulatory Network Models of Psychiatric and Neurodegenerative Disorders.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJocelynn R Pearl et. al.PubMed 30772379
- Translational psychiatry 8:2452018Convergence of independent DISC1 mutations on impaired neurite growth via decreased UNC5D expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPriya Srikanth et. al.PubMed 30410030
- Translational psychiatry 8:772018Shared effects of DISC1 disruption and elevated WNT signaling in human cerebral organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPriya Srikanth et. al.PubMed 29643329
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human POU3F2 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P20265
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Zebrafish | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Zebrafish | Dilution info - | Notes - |
Associated Products
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Target data
Function
Transcription factor that plays a key role in neuronal differentiation (By similarity). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity). Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (By similarity). Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (By similarity).
Alternative names
BRN2, OCT7, OTF7, Brain-specific homeobox/POU domain protein 2, Nervous system-specific octamer-binding transcription factor N-Oct-3, Octamer-binding protein 7, Octamer-binding transcription factor 7, Brain-2, Brn-2, Oct-7, OTF-7
Recommended products
Rabbit Polyclonal Brn-2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human POU3F2 aa 200 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human POU3F2 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P20265
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Brn-2 also known as POU domain class 3 transcription factor 2 (POU3F2) is a transcription factor with a molecular weight of approximately 48 kDa. This protein plays a significant role in the development and function of the central nervous system. It is abundantly expressed in neuronal tissues and specific types of melanocytes. Brn-2 belongs to the POU domain family of proteins which are pivotal in regulating gene expression during development.
- Biological function summary
Brn-2 regulates the expression of genes critical for neural differentiation and development. It acts as a transcriptional activator that binds to specific DNA sequences. Brn-2 does not act alone; it often participates in regulatory complexes with other transcription factors. Through these interactions Brn-2 influences the fate of neural progenitor cells and is involved in the regulation of neural stem cell maintenance.
- Pathways
Brn-2 plays a role in the Wnt signaling pathway and the Notch signaling pathway. Both of these pathways are integral to cell fate determination during development. In the Wnt pathway Brn-2 may interact with proteins such as β-catenin which modulates the expression of genes involved in cell proliferation and differentiation. Additionally Brn-2's role in the Notch pathway can influence the activity of proteins like RBPJ impacting cell communication and developmental processes.
- Associated diseases and disorders
Brn-2 has been linked to melanoma and gliomas. In melanoma Brn-2 can interact with other proteins such as MITF influencing melanocyte development and contributing to cancer progression. In glioma Brn-2's aberrant expression might affect cellular pathways that lead to uncontrolled proliferation and tumor formation. Understanding these connections provides insights into potential therapeutic targets for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Brn-2 antibody (ab137469)
10% SDS PAGE
All lanes: Western blot - Anti-Brn-2 antibody (ab137469) at 1/1000 dilution
All lanes: HCT116 whole cell lysate at 30 µg
Predicted band size: 46 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brn-2 antibody (ab137469)
Immunohistochemical analysis of paraffin embedded TOV-21G xenograft labelling Brn-2 with ab137469 antibody at a dilution of 1/500.
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